Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,970,526 G→C 7.0% R256R (CGC→CGG)  PP_5236 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,970,5260GC 7.0% 179.8 / 3.7 57R256R (CGC→CGG) PP_5236hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/28);  new base C (2/2);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

CGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACTGTG  >  NC_002947/5970381‑5970668
                                                                                                                                                 |                                                                                                                                              
cGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCgg                                                                                                                                             <  1:204626/149‑1 (MQ=255)
 gACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCggg                                                                                                                                            <  2:286310/149‑1 (MQ=255)
          cACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCTGCCCCGCTGGGGcggcag                                                                                                                                     <  1:357549/147‑1 (MQ=255)
           aCCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCggg                                                                                                                                            >  4:155556/1‑139 (MQ=255)
           aCCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCggg                                                                                                                                            <  3:155556/139‑1 (MQ=255)
            ccTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTa                                                                                                                                  >  5:162085/1‑148 (MQ=255)
                gTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTAcaca                                                                                                                              <  2:349641/148‑1 (MQ=255)
                   aGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACATCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCa                                                                                                                           <  2:317962/148‑1 (MQ=255)
                     ccTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTac                                                                                                                                 <  7:325182/140‑1 (MQ=255)
                     ccTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTac                                                                                                                                 >  8:325182/1‑140 (MQ=255)
                       tgtgGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGc                                                                                                                      >  6:357058/1‑149 (MQ=255)
                         tgGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCt                                                                                                                    >  3:306289/1‑149 (MQ=255)
                         tgGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCt                                                                                                                    <  2:107745/149‑1 (MQ=255)
                           gCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTg                                                                                                                   >  8:7718/1‑148 (MQ=255)
                           gCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTg                                                                                                                   >  7:332470/1‑148 (MQ=255)
                                   aGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCtt                                                                                                           <  1:274052/148‑1 (MQ=255)
                                    gCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTc                                                                                                          >  7:39736/1‑148 (MQ=255)
                                                    cATCCAGGGCAGCGCATTACGCAAATGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGgggcggg                                                                                         <  1:302556/149‑1 (MQ=255)
                                                        cagcgcagcgCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGAt                                                                                     <  8:39736/149‑1 (MQ=255)
                                                          gcgcagcgcaTTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCa                                                                                   <  6:10038/149‑1 (MQ=255)
                                                                     tACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGgcg                                                                         >  1:354137/1‑148 (MQ=255)
                                                                      aCGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGgcgc                                                                        <  4:64436/148‑1 (MQ=255)
                                                                       cGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCa                                                                       <  4:99564/148‑1 (MQ=255)
                                                                              gCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTaa                                                               >  3:227135/1‑149 (MQ=255)
                                                                                tCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTaa                                                               >  8:192821/1‑147 (MQ=255)
                                                                                  gTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAg                                                            <  4:227135/148‑1 (MQ=255)
                                                                                   tCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCg                                                                                                >  1:172104/1‑111 (MQ=255)
                                                                                   tCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCg                                                                                                <  2:172104/111‑1 (MQ=255)
                                                                                    cccAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGc                                                           >  3:288733/1‑147 (MQ=255)
                                                                                     ccAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAg                                                        <  7:192821/149‑1 (MQ=255)
                                                                                         gTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGc                                                           >  4:124668/1‑142 (MQ=255)
                                                                                         gTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGc                                                           <  3:124668/142‑1 (MQ=255)
                                                                                                 caGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGtttt                                            <  3:110284/149‑1 (MQ=255)
                                                                                                  aGCGGGCCATTGACCAACAGCTCGAACGCATCAACGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTc                                           <  4:288733/149‑1 (MQ=255)
                                                                                                      ggCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATgg                                       <  8:332470/149‑1 (MQ=255)
                                                                                                       gCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGt                                      >  7:98194/1‑149 (MQ=255)
                                                                                                         cATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCg                                    <  7:7718/149‑1 (MQ=255)
                                                                                                            tGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAagca                                                                                                    <  6:178586/82‑1 (MQ=255)
                                                                                                             gACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAgg                                                       >  1:180521/1‑126 (MQ=255)
                                                                                                             gACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAgg                                                       <  2:180521/126‑1 (MQ=255)
                                                                                                                         gAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCt                                                                                                                    <  3:179301/53‑1 (MQ=255)
                                                                                                                         gAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCt                                                                                                                    >  4:179301/1‑53 (MQ=255)
                                                                                                                         gAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGTGCGATCAGCGCATGGCGCATCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGAt                    <  5:357058/149‑1 (MQ=255)
                                                                                                                         gAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGa                     >  7:29490/1‑148 (MQ=255)
                                                                                                                         gAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGa                     >  7:46381/1‑148 (MQ=255)
                                                                                                                         gAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGa                     >  6:302171/1‑148 (MQ=255)
                                                                                                                         gAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGa                     <  4:306289/148‑1 (MQ=255)
                                                                                                                               aTCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGAAGCCAGTAATAGCGAGGGTACTCGTTTTCATGCTCGAACCGCTTGCCAAGATAGcgcg              >  8:242211/1‑149 (MQ=255)
                                                                                                                                tCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCgg             >  6:260485/1‑149 (MQ=255)
                                                                                                                                   aGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATa                                                             >  5:75563/1‑98 (MQ=255)
                                                                                                                                   aGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATa                                                             <  6:75563/98‑1 (MQ=255)
                                                                                                                                      ctgctgCCCCGCTGGGGCGGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGTGCGATCAGAGCATGGCGCAGCCAGTAATAGAGAGGGTCCTCGTTTTCAt                                         >  8:116863/1‑115 (MQ=255)
                                                                                                                                      ctgctgCCCCGCTGGGGCGGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCTTTTTCAt                                         <  7:116863/115‑1 (MQ=255)
                                                                                                                                      ctgctgCCCCGCTGGGGCGGCAGGTACACAGAAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCCCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGa       >  5:344772/1‑149 (MQ=255)
                                                                                                                                           gccCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACtgtg  <  6:162085/149‑1 (MQ=255)
                                                                                                                                           gccCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACtgtg  >  6:1381/1‑149 (MQ=255)
                                                                                                                                           gccCCAGCGGGGCAGCAGGTACAAAGCAAGGGCCTGGATCCCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATCGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACtgtg  >  5:14961/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CGACGGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGCGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCAGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACTGTG  >  NC_002947/5970381‑5970668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑