Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,976,577 A→G 7.4% A126A (GCA→GCG)  yifB → ATP‑dependent chelatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,976,5770AG 7.4% 151.5 / 2.7 54A126A (GCA→GCG) yifBATP‑dependent chelatase
Reads supporting (aligned to +/- strand):  ref base A (23/27);  new base G (2/2);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

TGGCGGCCGATATGACCTGGCCATCGCCCTGGGCATCCTCGCCGCCGACGGCCAGGTGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACCTGCTGGAGTTGGTCGCCCACCTGAACGGCCAGGTGCCGCTACCGCC  >  NC_002947/5976430‑5976723
                                                                                                                                                   |                                                                                                                                                  
tGGCGGCCGATATGACCTGGCCATCGCCCTGGGCATCCTCGCCGCCGACGGCCAGGTGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCAc                                                                                                                                                   >  1:240763/1‑149 (MQ=255)
                   GCCATCGCCCTGGGCATCCTCGCCGCCGACGGCCAGGTGCCGATTGCCACCCTGACCGATATCGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCa                                                                                                                                  >  4:102849‑M1/1‑147 (MQ=255)
                     CATCGCCCTGGGCATCCTCGCCGCCGACGGCCAGGTGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGcc                                                                                                                              <  1:11736‑M1/149‑1 (MQ=255)
                      aTCGCCCTGGGCATCCTCGCCGCCGACGGCCAGGTGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCg                                                                                                                             <  6:119498/149‑1 (MQ=255)
                               ggCATCCTCGCCGCCGACGGCCAGGTGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCtg                                                                                                                      <  6:269998/147‑1 (MQ=255)
                                gCATCCTCGCCGCCGACGGCCAGGTGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCtggtg                                                                                                                   <  1:326979/149‑1 (MQ=255)
                                 cATCCTCGCCGCCGACGGCCAGGTGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCtggtgg                                                                                                                  <  2:238579/149‑1 (MQ=255)
                                      tCGCCGCCGACGGCCAGGTGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCa                                                                                                             >  5:181713/1‑149 (MQ=255)
                                       gacctccgACGGCCAGGTGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCTGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCtggtg                                                                                                                   <  6:49741/137‑1 (MQ=255)
                                        gccgccGACGGCCAGGTGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACg                                                                                                           <  6:181713/149‑1 (MQ=255)
                                          cgccgACGGCCAGGTGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCGCTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACgg                                                                                                          >  3:190832/1‑148 (MQ=255)
                                          cgccgACGGCCAGGTGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCGCTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACgg                                                                                                          >  5:26925/1‑148 (MQ=255)
                                              gACGGCCAGGTGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGaaaa                                                                                                     >  7:117835/1‑149 (MQ=255)
                                                cGGCCAGGTGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATg                                                                                                   <  2:240763/149‑1 (MQ=255)
                                                cGGCCAGGTGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATg                                                                                                   <  1:358443/149‑1 (MQ=255)
                                                cGGCCAGGTGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGGGCAGGGCGGGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATg                                                                                                   <  2:290871/149‑1 (MQ=255)
                                                  gccaggtGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGcc                                                                                                 <  4:294703‑M2/142‑1 (MQ=255)
                                                   ccaggtGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGcc                                                                                                 >  1:319005‑M2/7‑148 (MQ=255)
                                                     aggtGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAg                                                                                              >  3:257450‑M2/5‑149 (MQ=255)
                                                                     ccTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAg                                                                                              <  7:8603/133‑1 (MQ=255)
                                                                     ccTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAg                                                                                              >  8:8603/1‑133 (MQ=255)
                                                                           cGATATGGAATGCCT‑GGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGtt                                                                       >  2:57196/1‑149 (MQ=255)
                                                                             atatGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTtg                                                                      >  6:295697/1‑149 (MQ=255)
                                                                             atatGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTtg                                                                      <  8:117835/149‑1 (MQ=255)
                                                                                tGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTtggtg                                                                   <  2:319005/149‑1 (MQ=255)
                                                                                  gAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTggtggt                                                                 <  8:216859/149‑1 (MQ=255)
                                                                                  gAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTggtggt                                                                 >  7:251141/1‑149 (MQ=255)
                                                                                  gAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTggtggt                                                                 >  1:135722/1‑149 (MQ=255)
                                                                                        cTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCCCGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTGGGTGGTGTATGc                                                           >  4:244067/1‑149 (MQ=255)
                                                                                        cTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCcggcg                                                                           <  8:102371/133‑1 (MQ=255)
                                                                                        cTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGc                                                           >  3:259526/1‑149 (MQ=255)
                                                                                        cTGGGCGAACTGGCCCTGTCGGGGAAATAGCGCCCGGTGCAGGGCGTGCTACATGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACCGGAAAATGCCGAGGAAGCCAGCCTGGCcggcg                                                                           >  7:102371/1‑133 (MQ=255)
                                                                                           ggCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGGGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGt                                                        <  5:311239/149‑1 (MQ=255)
                                                                                               aaCTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGc                                                    <  2:135722/149‑1 (MQ=255)
                                                                                                      ccTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCGCTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACctgc                                             <  6:26925/149‑1 (MQ=255)
                                                                                                      ccTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCGCTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACctgc                                             <  4:190832/149‑1 (MQ=255)
                                                                                                      ccTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACctgc                                             >  5:31834/1‑149 (MQ=255)
                                                                                                          tCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACctgctg                                           <  1:57196/147‑1 (MQ=255)
                                                                                                          tCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACCTGCTgg                                          <  5:295697/148‑1 (MQ=255)
                                                                                                             gggAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACCTGCTGGAGtt                                      >  7:187644/1‑149 (MQ=255)
                                                                                                              ggAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACCTGCTGGAGTTg                                     >  5:279019/1‑149 (MQ=255)
                                                                                                                 aaTTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACCTGCTGGAGTTGGTc                                  <  8:251141/149‑1 (MQ=255)
                                                                                                                            gTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACCTGCTGGAGTTGGTCGCCCACCTGaa                       >  8:222103/1‑149 (MQ=255)
                                                                                                                             tGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGa                                                                                               >  6:292357/1‑76 (MQ=255)
                                                                                                                             tGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGa                                                                                               <  5:292357/76‑1 (MQ=255)
                                                                                                                               cAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACCTGCTGGAGTTGGTCGCCCACCTGAACgg                    >  6:329347/1‑149 (MQ=255)
                                                                                                                                 gggCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACCTGCTGGAGTTGGTCGCCCACCTGAACGGcc                  <  8:187644/149‑1 (MQ=255)
                                                                                                                                   gCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACCTGCTGGAGTTGGTCGCCCACCTGAACGGCCAg                <  6:279019/149‑1 (MQ=255)
                                                                                                                                      tGCTACCTGCGGCACTGGCGGCACGCGAAGCCGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACCTGCTGGAGTTGGTCGCCCACCTGAACGGCCAGGTg             >  7:244336/1‑149 (MQ=255)
                                                                                                                                            cTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACCTGCTGGa                                         <  7:58772/115‑1 (MQ=255)
                                                                                                                                            cTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACCTGCTGGa                                         >  8:58772/1‑115 (MQ=255)
                                                                                                                                             tGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGTGGAAGCCAGCCTGGCCGGCGGGTtggtg                                                                   <  1:204227/88‑1 (MQ=255)
                                                                                                                                             tGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTtggtg                                                                   >  2:204227/1‑88 (MQ=255)
                                                                                                                                                 gCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACCTGCTGGAGTTGGTCGCCCACCTGAACGGCCAGGTGCCGCTACCGcc  <  7:222103/149‑1 (MQ=255)
                                                                                                                                                   aCTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCAc                                                                                                            <  2:266285/41‑1 (MQ=255)
                                                                                                                                                   aCTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCAc                                                                                                            >  1:266285/1‑41 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGGCGGCCGATATGACCTGGCCATCGCCCTGGGCATCCTCGCCGCCGACGGCCAGGTGCCGATTGCCACCCTGACCGATATGGAATGCCTGGGCGAACTGGCCCTGTCGGGGAAATTGCGCCCGGTGCAGGGCGTGCTACCTGCGGCACTGGCGGCACGCGAAGCAGGCCGGGCGCTGGTGGTACCACGGGAAAATGCCGAGGAAGCCAGCCTGGCCGGCGGGTTGGTGGTGTATGCGGTGGGCCACCTGCTGGAGTTGGTCGCCCACCTGAACGGCCAGGTGCCGCTACCGCC  >  NC_002947/5976430‑5976723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑