Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 417,317 A→T 7.3% S343C (AGC→TGC)  waaG → lipopolysaccharide glucosyltransferase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947417,3170AT 7.3% 152.5 / 2.8 55S343C (AGC→TGC) waaGlipopolysaccharide glucosyltransferase I
Reads supporting (aligned to +/- strand):  ref base A (27/23);  new base T (2/2);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

CGGCTTGCCGGTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATG  >  NC_002947/417169‑417463
                                                                                                                                                    |                                                                                                                                                  
cGGCTTGCCGGTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCa                                                                                                                                                    <  7:183011/149‑1 (MQ=255)
         ggTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTcgc                                                                                                                                           <  6:216697/149‑1 (MQ=255)
              tGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAAt                                                                                                                                      >  1:218119/1‑149 (MQ=255)
              tGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAAt                                                                                                                                      >  8:71507/1‑149 (MQ=255)
                gtgtCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATgg                                                                                                                                    >  3:64936/1‑149 (MQ=255)
                            tgCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGc                                                                                                                        >  4:304703/1‑149 (MQ=255)
                              cGGTTATGCCCACTACATTGCCGAGGCCGACAGCTGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTTCCAGCTGGTCGCGCAATGGCCTGGCATTTGCt                                                                                                                       <  3:263394/148‑1 (MQ=255)
                               ggTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGa                                                                                                                     <  7:71507/149‑1 (MQ=255)
                                 ttATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATa                                                                                                                   <  3:123146/149‑1 (MQ=255)
                                       ccACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGcc                                                                                                              >  1:250589/1‑148 (MQ=255)
                                         aCTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGa                                                                                                            >  3:191911/1‑148 (MQ=255)
                                         aCTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGAt                                                                                                           <  2:88683/149‑1 (MQ=255)
                                          cTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATc                                                                                                          >  6:308612/1‑149 (MQ=255)
                                              aTTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGc                                                                                                                              >  7:125649/1‑125 (MQ=255)
                                              aTTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGc                                                                                                                              <  8:125649/125‑1 (MQ=255)
                                              aTTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTa                                                                                                      <  2:218119/149‑1 (MQ=255)
                                                 gCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACa                                                                                                    <  1:243912/148‑1 (MQ=255)
                                                    gAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCAt                                                                                                >  1:148218/1‑149 (MQ=255)
                                                         cGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCgc                                                                                           >  4:248031/1‑149 (MQ=255)
                                                         cGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCg                                                                                            >  6:237095/1‑148 (MQ=255)
                                                             aGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAgcg                                                                                                                <  6:154557/124‑1 (MQ=255)
                                                             aGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAgcg                                                                                                                >  5:154557/1‑124 (MQ=255)
                                                              gCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCAt                                                                                      <  2:250589/149‑1 (MQ=255)
                                                                    tGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCTGCGGCAtgctg                                                                                  >  8:326191/1‑147 (MQ=255)
                                                                    tGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCTGCGGCAtgctg                                                                                  <  7:326191/147‑1 (MQ=255)
                                                                       tGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCACAGCATGCTGCCGAc                                                                             <  5:153217/149‑1 (MQ=255)
                                                                                 gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGCCCCGCAGGCGCGTGCCCGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGAACAGCATGCCGCAGCATGCTGCCGACGTGATCCTgg                                                                   >  3:20207/1‑149 (MQ=255)
                                                                                 gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAgcg                                                                                                                <  8:211476/104‑1 (MQ=255)
                                                                                 gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAgcg                                                                                                                >  7:211476/1‑104 (MQ=255)
                                                                                 gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTgg                                                                   >  2:355354/1‑149 (MQ=255)
                                                                                 gCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGGCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATTCTgg                                                                   <  4:64936/149‑1 (MQ=255)
                                                                                  ccGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACTCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTggg                                                                  >  1:88735/1‑149 (MQ=255)
                                                                                  ccGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTggg                                                                  <  7:260802/149‑1 (MQ=255)
                                                                                              gagCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCCCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGc                                                      <  5:308612/149‑1 (MQ=255)
                                                                                               agcagcTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCa                                                     <  5:23629/149‑1 (MQ=255)
                                                                                                 cagcTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCAt                                                    >  3:286456/1‑148 (MQ=255)
                                                                                                     tCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCAGATCTGTACAGCATGCCGCAGCATGCTGCAGACCTGATCCTGGGGCAAAGAACCGCATGAAGc                                               >  8:8472/1‑149 (MQ=255)
                                                                                                         cGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGat                                           >  1:353359/1‑149 (MQ=255)
                                                                                                          ggCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTgggg                                                                 <  8:327227/126‑1 (MQ=255)
                                                                                                          ggCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTgggg                                                                 >  7:327227/1‑126 (MQ=255)
                                                                                                               tCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTg                                       <  4:191911/147‑1 (MQ=255)
                                                                                                                   cAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGc                                     <  5:237095/145‑1 (MQ=255)
                                                                                                                          tGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCa                          <  2:88735/149‑1 (MQ=255)
                                                                                                                           gCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCa                          >  3:213830/1‑148 (MQ=255)
                                                                                                                               cgcgACCAGCTGGCACGCGCCTGCGGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGc                     >  8:100678/4‑149 (MQ=255)
                                                                                                                                gcgACCAGCTGGCACGCGCCTGCGGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGc                                               <  4:80502/120‑1 (MQ=255)
                                                                                                                                gcgACCAGCTGGCACGCGCCTGCGGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGc                                               >  3:80502/3‑122 (MQ=255)
                                                                                                                                   aCCAGCTGGCACGCGCCTGCGGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCtgt                 <  7:100678/149‑1 (MQ=255)
                                                                                                                                      cGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGCCGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTggg              >  7:125396/1‑149 (MQ=255)
                                                                                                                                           gcgcGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCggg         >  7:250514/1‑149 (MQ=255)
                                                                                                                                               gTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCg           >  1:177939/1‑143 (MQ=255)
                                                                                                                                                 gCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGAt   >  2:220453/1‑149 (MQ=255)
                                                                                                                                                  ccAGCTGGTCTCGCAATGGCCTGTCATTTGCTGATAGCGCCGATCTTTACAGAATGCCGCAGCATGCTGCCGACCTTATCCTGGTGCAAAAAAGCGCATGAAGCTGATATTGGACGAACCCTTCAAACGCCTGTGGGCCGGGc        <  7:161031/143‑1 (MQ=255)
                                                                                                                                                  ccAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGAt   <  1:355354/148‑1 (MQ=255)
                                                                                                                                                  ccAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCAGAACCCTTCAAACGCCTGTGGGCCGGGCgacatc  >  8:161031/1‑144 (MQ=255)
                                                                                                                                                   cAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATg  >  7:301966/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGGCTTGCCGGTGCTGGTGTCCAAGGTGTGCGGTTATGCCCACTACATTGCCGAGGCCGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATG  >  NC_002947/417169‑417463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑