Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,994,761	G→C	15.0%	V496V (GTG→GTC)	PP_5251 →	transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,994,761	0	G	C	15.0%	97.4 / 11.4	40	V496V (GTG→GTC)	PP_5251	transporter
Reads supporting (aligned to +/- strand):  ref base G (13/21);  new base C (2/4);  total (15/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.81e-01

GCAGGCGGCGCGGGAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGCCCATTCGCGGGTGAAC  >  NC_002947/5994614‑5994904                                                                                                                                                    |                                                                                                                                                 gcagGCGGCGCGGGAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGa                                                                                                                                                 >  6:216395/1‑149 (MQ=255)       ggcgCGGTAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑gtg                                                                                                                                          <  1:122350/149‑1 (MQ=255)       ggcgCGGGAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCA‑CGGGTCACCC‑ggtg                                                                                                                                         >  4:352170/1‑146 (MQ=255)        gcgcGGGAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑gtgg                                                                                                                                         <  7:178584/149‑1 (MQ=255)          gcgGGAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑gtggt                                                                                                                                        >  7:149340/1‑148 (MQ=255)          gcgGGAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCA‑CGGGTCACCCGgtggtg                                                                                                                                       <  2:79453/149‑1 (MQ=255)          gcgGGAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCA‑CGGGTCACCCGgtggtg                                                                                                                                       <  3:352170/149‑1 (MQ=255)            gggAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGaa                                                                                                                                     <  4:291384/149‑1 (MQ=255)             ggAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAAc                                                                                                                                    <  1:284466/149‑1 (MQ=255)              gAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCA‑CGGGTCACCCGGTGGTGatgt                                                                                                                                   <  3:45992/149‑4 (MQ=255)                    gAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAg                                                                                                                               <  5:197158/147‑1 (MQ=255)                      aGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCg                                                                                                                           <  1:275373/149‑1 (MQ=255)                        cGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAg                                                                                                                         <  8:149340/149‑1 (MQ=255)                        cGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAg                                                                                                                         <  5:51666/149‑1 (MQ=255)                             cgcaagcgcaaTGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGgcgc                                                                                                                     <  2:13568/148‑1 (MQ=255)                                        tGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGAtgc                                                                                                         <  4:252275/149‑1 (MQ=255)                                        tGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGAtgc                                                                                                         <  1:44753/149‑1 (MQ=255)                                                          gTTGATGCGGGACAGTGCTGATGCGGCGTTGTGGCTGGTGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACGGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTGTGGATGCTGCTGACCGATGTGGTTg                                                                                       <  2:57281/149‑1 (MQ=255)                                                               tGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTg                                                                                  >  7:76738/1‑149 (MQ=255)                                                               tGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTg                                                                                  >  6:253097/1‑149 (MQ=255)                                                                          gCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAg                                                                                     >  6:190162/1‑135 (MQ=255)                                                                          gCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAg                                                                                     <  5:190162/135‑1 (MQ=255)                                                                             gATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATc                                                                     >  6:323905/1‑148 (MQ=255)                                                                               tCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAAGCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTAt                                                                  <  5:216395/149‑1 (MQ=255)                                                                                 cGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTg                                                                >  1:91269/1‑149 (MQ=255)                                                                                   gCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGc                                                              <  3:18091/149‑1 (MQ=255)                                                                                     gTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCtg                                                            >  5:240859/1‑149 (MQ=255)                                                                                              ggAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGa                                                                                      <  7:2346/114‑1 (MQ=255)                                                                                              ggAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGa                                                                                      >  8:2346/1‑114 (MQ=255)                                                                                                          cGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCacac                                        >  4:3196/1‑148 (MQ=255)                                                                                                             gcaaTCGTGGCTGCACCCTCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTTGTGGGGAGCATAAGGCACACCAtt                                    <  4:325088/147‑1 (MQ=255)                                                                                                                          gAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGccc                         <  2:91269/147‑1 (MQ=255)                                                                                                                                   cGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGc                  <  5:253097/145‑1 (MQ=255)                                                                                                                                      gttCACCA‑CGGGTCACCCGGTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACgg                   >  4:165978/3‑141 (MQ=255)                                                                                                                                      gttCACCA‑CGGGTCACCCGGTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACgg                   <  3:165978/139‑1 (MQ=255)                                                                                                                                         caccacCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGCCCATTCGCgg        >  5:75304/1‑149 (MQ=255)                                                                                                                                           ccaccGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGCCCATTCGCGGGt      >  6:104596/1‑149 (MQ=255)                                                                                                                                            caccGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGa                                  >  1:135189/1‑120 (MQ=255)                                                                                                                                            caccGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGa                                  <  2:135189/120‑1 (MQ=255)                                                                                                                                               cGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGTGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGCCCATTCGCGGGTGAAc  <  8:76738/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 GCAGGCGGCGCGGGAGCTGGAAGCGATGCGCAAGCGCAATGGCGCATTGTCGTGGGGGTTGATGCGGGACAGTGCTGATCCGGCGTTGTGGCTGGAGTTCTTCTTCGAGGAATCGTGGCTGGAGCATCTGCGACATCACCACCGGGTGACCC‑GTGGTGAACTGAAGATCGAGGCGCGGGTCAGGATGCTGCAGACCGATGGGGTTGAGGTGCGGATTCGGCATCTATTGGCTGGTGGGGAGCATAAGGCACACCATTGATGCCGGCCCTTACGGCCCATTCGCGGGTGAAC  >  NC_002947/5994614‑5994904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑