Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,003,644 C→A 8.2% G98V (GGC→GTC)  amaB ← L‑piperidine‑6‑carboxylate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,003,6440CA 8.2% 138.2 / 2.1 45G98V (GGC→GTC) amaBL‑piperidine‑6‑carboxylate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (19/22);  new base A (2/2);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCACCAGTTCGCCACGGCGCGGAGCCGGCACACTGCGCCAGGCTTCGAAGGCGCTCTGG  >  NC_002947/6003502‑6003787
                                                                                                                                              |                                                                                                                                               
cACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCt                                                                                                                                           >  5:226956/1‑149 (MQ=255)
       aCCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGAAGGCCTGGGTGaa                                                                                                                                    <  5:120485/149‑2 (MQ=255)
            acCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTgg                                                                                                                                         >  8:136493/1‑139 (MQ=255)
            acCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTgg                                                                                                                                         <  7:136493/139‑1 (MQ=255)
            acCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGc                                                                                                                               >  2:214751/1‑149 (MQ=255)
               cAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCgg                                                                                                                            <  6:260198/149‑1 (MQ=255)
                     gTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTtcga                                                                                                                      >  7:43017/1‑149 (MQ=255)
                           ggTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCgaga                                                                                                                <  4:289534/149‑1 (MQ=255)
                            gTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGAc                                                                                                               <  4:346288/149‑1 (MQ=255)
                             tttCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGAAGGCCTGGGTGa                                                                                                                                     <  3:338198/126‑1 (MQ=255)
                             tttCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGAAGGCCTGGGTGa                                                                                                                                     >  4:338198/1‑126 (MQ=255)
                                        tggtggCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAg                                                                                                   <  1:214751/149‑1 (MQ=255)
                                            ggCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCg                                                                                               >  2:357810/1‑149 (MQ=255)
                                             gCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCgg                                                                                              <  8:43017/149‑1 (MQ=255)
                                               ccGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGcc                                                                                            >  7:143752/1‑149 (MQ=255)
                                                     gcTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGc                                                                                      >  2:219721/1‑149 (MQ=255)
                                                          gAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACg                                                                                 <  6:226956/149‑1 (MQ=255)
                                                                aTGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTtcgatc                                                                                                                    >  7:201531/1‑108 (MQ=255)
                                                                aTGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTtcgatc                                                                                                                    <  8:201531/108‑1 (MQ=255)
                                                                       ggCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGcc                                                                                            <  5:115348/125‑1 (MQ=255)
                                                                       ggCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGcc                                                                                            >  6:115348/1‑125 (MQ=255)
                                                                         ccGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGaa                                                                  >  8:156049/1‑149 (MQ=255)
                                                                               aGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGgcg                                                            <  4:239689/149‑1 (MQ=255)
                                                                                  tGGCGCGACAGGTCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGa                                                                   <  5:289175/139‑1 (MQ=255)
                                                                                  tGGCGCGACAGGTCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGa                                                                   >  6:289175/1‑139 (MQ=255)
                                                                                  tGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCAc                                                         >  5:360307/1‑149 (MQ=255)
                                                                                  tGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCAc                                                         >  6:285359/1‑149 (MQ=255)
                                                                                   ggCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCTGCACTTCGCCGAACAGGCGCAc                                                         >  2:30192/1‑148 (MQ=255)
                                                                                    gcgcGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCAc                                                         <  1:357810/147‑1 (MQ=255)
                                                                                          cAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCTGCACTTCGCCGAACAGGCGCACCAGTTCGc                                                 <  1:30192/149‑1 (MQ=255)
                                                                                            ggCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCACCAGTTCGCCa                                               <  4:19729/149‑1 (MQ=255)
                                                                                                         gTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACAAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCACCAGTTCGCCACGGCGCGGAGCCg                                  >  3:58515/1‑149 (MQ=255)
                                                                                                          tCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCACCAGTTCGCCACGGCGCGGAGCCgg                                 <  5:272285/149‑1 (MQ=255)
                                                                                                                   tCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCACCAGTTCGCCACGGCGCGGAGCCGGCACACTgcg                        >  5:72246/1‑149 (MQ=255)
                                                                                                                   tCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCACCAGTTCGCCACGGCGCGGAGCCGGCACACTgc                         <  6:360307/148‑1 (MQ=255)
                                                                                                                    cGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCACCAGTTCGCCACGGCGCGGAGCCGGCACACTgcgc                       <  7:156049/149‑1 (MQ=255)
                                                                                                                     gATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCACCAGTTCGCCACGGCGCGGAGCCGGCACACTGCGcc                      <  5:181669/149‑1 (MQ=255)
                                                                                                                         aTTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCACCAGTTCGCCACGGCGCGGAGCCGGCACACTGCGCCAGGc                  <  1:219721/149‑1 (MQ=255)
                                                                                                                         aTTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCACCAGTTCGCCACGGCGCGGAGCCGGCACACTGCGCCAGGc                  >  7:164418/1‑149 (MQ=255)
                                                                                                                         aTTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCACCAGTTCGCCACGGCGCGGAGCCGGCACACTGCGCCAGGc                  >  6:347238/1‑149 (MQ=255)
                                                                                                                              ctccACTTCACCCAGGACTTCCTGCGTGATCTTGACGGCTTCGATCGAGACAACCACGCCGAGCTCCGCCATGTGCTCACGCAGCACTTCGCCGAACACGAGCACCAGTTCGCCACGGCGCGGAGCCGGCACACTGCGCCAGGCTTCg              >  4:55568/4‑148 (MQ=255)
                                                                                                                              cTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCACCAGTTCGCCACGGCGCGGAGCCGGCACACTGCGCCAGGCTTCGa             <  8:143752/149‑1 (MQ=255)
                                                                                                                                      cACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCACCAGTTCGCCACGGCGCGGAGCCGGCACACTGCGCCAGGCTTCGa             >  1:84083/1‑141 (MQ=255)
                                                                                                                                      cACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCACCAGTTCGCCACGGCGCGGAGCCGGCACACTGCGCCAGGCTTCGa             <  2:84083/141‑1 (MQ=255)
                                                                                                                                         ccAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCACCAGTTCGCCACGGCGCGGAGCCGGCACACTGCGCCAGGCTTCGAAGGCGCTCTgg  <  1:246195/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
CACGCCGACCACACCCAGCGGGTGCCAGGTTTCACGCATGTGGTGGCCCGGGCGCTCGGAAGCGATGGTCAGGCCGTACAGCTGGCGCGACAGGCCAACGGCGAAGTCGCAGATGTCGATCATTTCCTGCACTTCACCCAGGCCTTCCTGGGTGATCTTGCCGGCTTCGATCGAGACCAGCTCGCCGAGGTCGGCCTTGTGCTCACGCAGCACTTCGCCGAACAGGCGCACCAGTTCGCCACGGCGCGGAGCCGGCACACTGCGCCAGGCTTCGAAGGCGCTCTGG  >  NC_002947/6003502‑6003787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑