Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,075,747 G→C 6.9% A272G (GCC→GGC)  PP_5328 ← phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,075,7470GC 6.9% 214.5 / 3.5 69A272G (GCC→GGC) PP_5328phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (29/34);  new base C (2/3);  total (32/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCCCCCCTTGGAGTCACCGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGACCAC  >  NC_002947/6075606‑6075895
                                                                                                                                              |                                                                                                                                                     
aGCCCCCCCTAGGAGTCACCGATGATCAGCGAAAAGCCGCCAACCAGCTCCGCCGTCGCAGAAAATTCGGTCTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCCGCCTGACAACTTCCTCCGTGTCAC‑Gccc                                                                                                                                               >  8:326981/1‑149 (MQ=255)
   ccccccTTGGAGTCACCGATGGTCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGtt                                                                                                                                            <  3:308513/149‑1 (MQ=255)
   ccccccTTGGAGTCACCGATGATCAGCGAAATGCCGCCAACCATCTGGGCCGTGGCAGTAATTTCGGGGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGtt                                                                                                                                            <  3:16405/149‑1 (MQ=255)
       ccTTGGAGTCACCGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGGCCCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGAt                                                                                                                                         <  1:98411/149‑1 (MQ=255)
       ccTTGGAGTCACCGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGAt                                                                                                                                         >  7:56048/1‑148 (MQ=255)
        cTTGGAGTCACCGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGCCAC‑GGCCgacgtgtt                                                                                                                                       <  2:315150/149‑8 (MQ=255)
                  ccGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGt                                                                                                                                             <  5:44896/133‑1 (MQ=255)
                  ccGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGt                                                                                                                                             >  6:44896/1‑133 (MQ=255)
                   cGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGc                                                                                                                               >  4:352617/1‑146 (MQ=255)
                   cGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGc                                                                                                                               <  3:352617/146‑1 (MQ=255)
                           aGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTg                                                                                                                       >  5:183168/1‑146 (MQ=255)
                           aGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTg                                                                                                                       <  6:183168/146‑1 (MQ=255)
                           aGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGc                                                                                                                     <  8:174577/148‑1 (MQ=255)
                            gCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGt                                                                                                                   >  6:73023/1‑149 (MQ=255)
                            gCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGt                                                                                                                   <  4:311102/149‑1 (MQ=255)
                             cGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACCACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGGACAGCCAGTGCTGGCGTg                                                                                                                  <  7:164620/149‑1 (MQ=255)
                             cGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTg                                                                                                                  >  8:81338/1‑149 (MQ=255)
                                aaTGCCGCCAACCAGCTGGGCCGTGGCAATAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGt                                                                                                               <  3:212719/149‑1 (MQ=255)
                                    ccgccAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGAt                                                                                                           <  7:155043/149‑1 (MQ=255)
                                     cgccAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGCCAC‑GGCCga                                                                                                                                             >  2:62427/1‑113 (MQ=255)
                                     cgccAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGCCAC‑GGCCga                                                                                                                                             <  1:62427/114‑2 (MQ=255)
                                      gccAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGa                                                                                                         <  7:65547/149‑1 (MQ=255)
                                      gccAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGCCAC‑GGCCga                                                                                                                                             <  4:323746/113‑2 (MQ=255)
                                      gccAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGCCAC‑GGCCga                                                                                                                                             >  3:323746/1‑112 (MQ=255)
                                       ccAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGtt                                                                                                                                            >  5:172644/1‑113 (MQ=255)
                                       ccAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGtt                                                                                                                                            <  6:172644/113‑1 (MQ=255)
                                       ccAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGa                                                                                                            >  6:44193/1‑145 (MQ=255)
                                       ccAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGa                                                                                                            <  5:44193/145‑1 (MQ=255)
                                                  ggCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATAttcttc                                                                                              <  8:56048/148‑1 (MQ=255)
                                                  ggCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGGCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATAttctt                                                                                               <  6:262179/147‑1 (MQ=255)
                                                         gCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCAGTGGAAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGtctc                                                                                      <  7:326981/149‑1 (MQ=255)
                                                           aGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCg                                                                                                             <  2:188493/124‑1 (MQ=255)
                                                           aGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCg                                                                                                             >  1:188493/1‑124 (MQ=255)
                                                           aGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAAAACGTCGGCCGTGGAAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTAGATGAAGATATTCTTCACCGTCTCgg                                                                                    >  7:275731/1‑149 (MQ=255)
                                                            gTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGt                                                                                   >  3:191164/1‑149 (MQ=255)
                                                             tAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGt                                                                                   >  3:160042/1‑148 (MQ=255)
                                                                  tctgtgtCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTATTGTCGCGAAGTCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGTTCGATGAAGATATTCTTCACCGTCTCGGTCAttt                                                                              <  1:169857/145‑1 (MQ=255)
                                                                    ggTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCg                                                                           >  1:198206/1‑149 (MQ=255)
                                                                          ggCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGt                                                                      >  5:203043/1‑148 (MQ=255)
                                                                            cgcTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCg                                                                   <  5:73023/149‑1 (MQ=255)
                                                                                 tCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATg                                                                <  6:203043/147‑1 (MQ=255)
                                                                                          ttGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTTATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTc                                                     >  4:135577/1‑149 (MQ=255)
                                                                                            gTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCgg                                                   >  4:57920/1‑149 (MQ=255)
                                                                                             tAACGT‑CCGTGGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTGCTGCTCggt                                                  <  8:275731/149‑1 (MQ=255)
                                                                                              aaCGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCg                                                              <  8:8526/136‑1 (MQ=255)
                                                                                              aaCGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCg                                                              >  7:8526/1‑136 (MQ=255)
                                                                                               aCGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCACGGGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCggtg                                                 >  1:89477/1‑149 (MQ=255)
                                                                                               aCGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCggtgg                                                >  4:310544/1‑149 (MQ=255)
                                                                                               aCGG‑CCGGTGAGGCGCTTGTCGCGCACGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGGACAGCCAGGGCTGGCGTGGGTCGATGAAGATAGTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCggtgg                                                <  6:36001/149‑1 (MQ=255)
                                                                                                 gg‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCggtggt                                               >  7:58181/1‑148 (MQ=255)
                                                                                                    ccGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGAc                                            <  4:160042/149‑1 (MQ=255)
                                                                                                    ccGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGAc                                            <  8:58181/149‑1 (MQ=255)
                                                                                                          aGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTc                                      >  8:83282/1‑149 (MQ=255)
                                                                                                           ggCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCg                                     <  1:140875/149‑1 (MQ=255)
                                                                                                            gCTCGTGTCGCGCAGGCTGAACACGTCGGCCGTGGCCA‑GGCCGTTGATCACGTACAGCCAGTGCGGGCGTGGGTCGATGCAGATATTCTTCACCGTCTCGGGCATTTGCGGCAGTGCGCTGCGGGTCTGCTCGGTGGTGACctcggcgg                                    >  6:63258/1‑144 (MQ=255)
                                                                                                             ctctTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGt                                   <  7:81338/149‑1 (MQ=255)
                                                                                                                     gcgcAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGt                           >  3:54180/1‑149 (MQ=255)
                                                                                                                      cgcAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGtt                          >  5:332391/1‑149 (MQ=255)
                                                                                                                         aGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTc                       <  4:191164/149‑1 (MQ=255)
                                                                                                                             tGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTcc                      <  6:6818/146‑1 (MQ=255)
                                                                                                                             tGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTcc                      >  5:6818/1‑146 (MQ=255)
                                                                                                                                acacGTCGGCCTTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCgg                <  5:63258/149‑1 (MQ=255)
                                                                                                                                acacGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGTTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGc                  >  2:338680/1‑147 (MQ=255)
                                                                                                                                acacGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGTTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGc                  <  1:338680/147‑1 (MQ=255)
                                                                                                                                acacGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCgg                <  3:57920/149‑1 (MQ=255)
                                                                                                                                 cacGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCggg               >  8:79983/1‑149 (MQ=255)
                                                                                                                                       ggCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGc          <  8:189072/148‑1 (MQ=255)
                                                                                                                                           gTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGac     >  7:235740/1‑149 (MQ=255)
                                                                                                                                           gTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGT‑GGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGacc    >  6:253635/1‑149 (MQ=255)
                                                                                                                                              gCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGaccac  <  2:89477/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                     
AGCCCCCCCTTGGAGTCACCGATGATCAGCGAAATGCCGCCAACCAGCTGGGCCGTGGCAGTAATTTCGGTGTCGGCGCTATCAGACAACTTGTAACGG‑CCGTTGAGGCTCTTGTCGCGCAGGCTGAACACGTCGGCCGTGGCAC‑GGCCGTTGATCACGTACAGCCAGTGCTGGCGTGGGTCGATGAAGATATTCTTCACCGTCTCGGTCATTTGCGGCAGTTCGATGCGGTTCTGCTCGGTGGTGACCTCTTCGGTCATCATGTTTTCCGTGCGGGTCAGCTCGACCAC  >  NC_002947/6075606‑6075895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑