Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,101,852 A→T 7.1% Y335F (TAT→TTT) ‡ uvrD → DNA‑dependent ATPase I/helicase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,101,8520AT 7.1% 162.0 / 3.1 56Y335F (TAT→TTT) ‡uvrDDNA‑dependent ATPase I/helicase II
Reads supporting (aligned to +/- strand):  ref base A (25/27);  new base T (2/2);  total (27/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

CCGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTGCGCGAGCGCATCCCCTACCGCATCTATGG  >  NC_002947/6101706‑6101990
                                                                                                                                                   |                                                                                                                                           
ccGCTCCACCGGCTGCATCCTCAAGGCGGCCAACGCGGTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTAt                                                                                                                                            <  4:122945/148‑1 (MQ=255)
 cGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGt                                                                                                                                          >  8:215844/1‑149 (MQ=255)
 cGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGt                                                                                                                                          >  6:239802/1‑149 (MQ=255)
   cTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCg                                                                                                                                        >  2:74761/1‑149 (MQ=255)
      cACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTgg                                                                                                                                     <  2:220470/149‑1 (MQ=255)
          ggcggcATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAAc                                                                                                                                 >  4:124075/1‑149 (MQ=255)
           gcggcATCCTCAAGGCGGCCAACGCGATGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAAc                                                                                                                                 >  5:142800/1‑148 (MQ=255)
              gcATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATc                                                                                                                             >  2:254615/1‑149 (MQ=255)
                   cTCAAGGCGGCCAACGCGCTGATCGCCAACAACATCTGGCGCCTGGGCAAGGAACTGTGGACCGACATTGGCTAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACAATCgaga                                                                                                                         <  2:248695/148‑1 (MQ=255)
                    tCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGc                                                                                                                       >  2:265182/1‑149 (MQ=255)
                    tCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGc                                                                                                                       >  3:266836/1‑149 (MQ=255)
                       aGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTg                                                                                                                    <  3:124075/149‑1 (MQ=255)
                          cggcCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCCACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGt                                                                                                                  <  1:74761/149‑1 (MQ=255)
                          cggcCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATCGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTc                                                                                                                 <  4:339340/149‑1 (MQ=255)
                             ccAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑g                                                                                                             >  6:54020/1‑149 (MQ=255)
                                 cgcgCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAgg                                                                                                         <  7:215844/149‑1 (MQ=255)
                                        aTCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATgc                                                                                                  <  1:254615/149‑1 (MQ=255)
                                           gCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATgcgc                                                                                                <  4:266836/148‑1 (MQ=255)
                                            ccAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGCCCCTGTACGCGGCCTACTACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATgcgcg                                                                                               >  6:112239/1‑148 (MQ=255)
                                              aacaacAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGc                                                                                                       >  2:288028/1‑138 (MQ=255)
                                              aacaacAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGc                                                                                                       <  1:288028/138‑1 (MQ=255)
                                                 aacaGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAATGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACga                                                                                         <  6:65455/149‑1 (MQ=255)
                                                    aGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGAt                                                                                      <  5:54020/149‑1 (MQ=255)
                                                           gcCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACga                                                                                         >  5:92193/1‑139 (MQ=255)
                                                           gcCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACga                                                                                         <  6:92193/139‑1 (MQ=255)
                                                           gcCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATc                                                                               >  2:225720/1‑149 (MQ=255)
                                                           gcCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATc                                                                               >  3:40896/1‑149 (MQ=255)
                                                           gcCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATc                                                                               <  1:265182/149‑1 (MQ=255)
                                                             cTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGc                                                                                   <  2:101762/143‑1 (MQ=255)
                                                             cTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGc                                                                                   >  1:101762/1‑143 (MQ=255)
                                                             cTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCt                                                                             <  1:224988/149‑1 (MQ=255)
                                                                     ggAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATgcg                                                                                                 >  1:120492/1‑121 (MQ=255)
                                                                     ggAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATgcg                                                                                                 <  2:120492/121‑1 (MQ=255)
                                                                           gtgGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACg                                                               >  4:199920/1‑149 (MQ=255)
                                                                              gACCGACATGGGCGAAGGCGAACCACTGCCCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCCATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGcc                                                             >  2:277781/1‑148 (MQ=255)
                                                                                    cATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTcgc                                                      <  5:239802/149‑1 (MQ=255)
                                                                                     aTGGGCGAAGGGGAACCACTGACCGTGTACGCGGCCTACAACGAT‑CACGACGAAGCGCGCTATGTGGTGGAAACCATAGAGAGCCTGGTCAACTCAGGGCAATGCGCGGAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTcgc                                                      <  1:277781/149‑1 (MQ=255)
                                                                                      tGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTcgcgc                                                    <  5:112239/149‑1 (MQ=255)
                                                                                         gcgaaggcgaaCCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTg                                                 >  1:247161/1‑149 (MQ=255)
                                                                                                 gaaCCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGaa                                           >  3:3010/1‑147 (MQ=255)
                                                                                                      aCTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGcc                                                             >  3:306390/1‑124 (MQ=255)
                                                                                                      aCTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGcc                                                             <  4:306390/124‑1 (MQ=255)
                                                                                                             cTGTACGCGGCCTACAACGAG‑CACGACGCAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGGCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCGGGAAGAGGCCcagcatga                             <  4:12612/149‑5 (MQ=255)
                                                                                                                tACGCGGCCTACAACGAG‑CACTACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTgcgc                            <  6:142800/147‑1 (MQ=255)
                                                                                                                  cgcgGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTGCGCGAgc                        <  3:199920/149‑1 (MQ=255)
                                                                                                                   gcgGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTGCGCGAgcg                       >  7:91891/1‑149 (MQ=255)
                                                                                                                       ccTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTGCGCGAGCGCATc                   <  4:40896/149‑1 (MQ=255)
                                                                                                                          aCAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTACAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTGCGCGAGCGCATccc                 >  7:142342/1‑148 (MQ=255)
                                                                                                                           cAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTGCGCGAGCGCATccc                 <  4:76539/147‑1 (MQ=255)
                                                                                                                           cAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTGCGCGAGCGCATccc                 >  3:76539/1‑147 (MQ=255)
                                                                                                                               tttccACGACATAGCGCGCTTCGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTcgc                                                      <  8:266513/103‑1 (MQ=38)
                                                                                                                               tttccACGACATAGCGCGCTTCGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTcgc                                                      <  8:261727/103‑1 (MQ=38)
                                                                                                                               tttccACGACATAGCGCGCTTCGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTcgc                                                      >  7:266513/5‑107 (MQ=38)
                                                                                                                               tttccACGACATAGCGCGCTTCGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTcgc                                                      >  7:261727/5‑107 (MQ=38)
                                                                                                                                     cgacgaAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTGCGCGAGCGCATCCCCTACCGCATCt      <  1:225720/149‑1 (MQ=255)
                                                                                                                                         gaAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTGCGCGAGCGCATCCCCTACCGCATCTATgg  >  6:61002/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
CCGCTCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAG‑CACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAA‑GCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTGCGCGAGCGCATCCCCTACCGCATCTATGG  >  NC_002947/6101706‑6101990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑