Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,111,006 A→T 9.4% D168E (GAT→GAA) ‡ PP_5361 ← protein P47K

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,111,0060AT 9.4% 177.8 / 8.3 64D168E (GAT→GAA) ‡PP_5361protein P47K
Reads supporting (aligned to +/- strand):  ref base A (27/31);  new base T (3/3);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

CACTTGGCCCATGACCATGGGTACGATCTGCGCTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCT  >  NC_002947/6110859‑6111154
                                                                                                                                                   |                                                                                                                                                    
cACTTGGCCCATGACCATGGGTACGATCTGCGCTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAAt                                                                                                                                                     <  6:226518/149‑1 (MQ=255)
  cTTGGCCCATGACCATGGGTACGATCTGCGCTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCa                                                                                                                                                   <  6:83746/149‑1 (MQ=255)
     ggCCCATGACCATGGGTACGATCTGCGCTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCAc                                                                                                                                                  <  6:158260/147‑1 (MQ=255)
     ggCCCATGACCATGGGTACGATCTGCGCTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACt                                                                                                                                                 >  3:195608/1‑148 (MQ=255)
                aTGGGTACGATCTGCGCTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTc                                                                                                                                     <  6:44264/149‑1 (MQ=255)
                 tGGGTACGATCTGCGCTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCg                                                                                                                                    >  8:174116/1‑149 (MQ=255)
                  gggTACGATCTGCGCTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGt                                                                                                                                   >  2:159100/1‑149 (MQ=255)
                   ggTACGATCTGCGCTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGtc                                                                                                                                  <  3:245085/149‑1 (MQ=255)
                      aCGATCTGCGCTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGTGATTTGCTGATCGagc                                                                                                                                         >  1:12568/1‑137 (MQ=255)
                      aCGATCTGCGCTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGTGATTTGCTGATCGagc                                                                                                                                         <  2:12568/139‑3 (MQ=255)
                       cGATCTGCGCTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGtcttct                                                                                                                              <  5:276618/149‑1 (MQ=255)
                        gATCTGCGCTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGTGATTTGCTGATCGagcag                                                                                                                                       <  8:177494/139‑5 (MQ=255)
                        gATCTGCGCTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGTGATTTGCTGATCGagcag                                                                                                                                       >  7:177494/1‑135 (MQ=255)
                          tCTGCGCTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGTGATTTGCTGATCGag                                                                                                                                          >  8:154344/1‑133 (MQ=255)
                          tCTGCGCTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGTGATTTGCTGATCGag                                                                                                                                          <  7:154344/134‑2 (MQ=255)
                               gcTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGAACAGCAAATAACTGATCGACCGCTCGTCTTCTTCGCCCaa                                                                                                                      >  4:271598/1‑149 (MQ=255)
                                cTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAAc                                                                                                                     <  6:304081/149‑1 (MQ=255)
                                    ggCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGttt                                                                                                                 >  6:114815/1‑149 (MQ=255)
                                     gCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTc                                                                                                                <  5:114815/149‑1 (MQ=255)
                                           agaCTGCGCAAAATAGCGATGAGTTCTTCACGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCg                                                                                                          >  1:209193/1‑149 (MQ=255)
                                                    aaaaTAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAg                                                                                                 >  6:369742/1‑149 (MQ=255)
                                                     aaaTAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAgg                                                                                                <  7:174116/149‑1 (MQ=255)
                                                         aGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGt                                                                                            >  3:279002/1‑149 (MQ=255)
                                                           cGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTct                                                                                          >  4:284842/1‑149 (MQ=255)
                                                             aTGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTc                                                                                                                <  1:313404/125‑1 (MQ=255)
                                                             aTGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTc                                                                                                                >  2:313404/1‑125 (MQ=255)
                                                                aGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCtgctgc                                                                                     <  4:126177/149‑1 (MQ=255)
                                                                 gTTCTTCGCGCTCATGTTGGCTGATCAGGTCTATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGcc                                                                                    <  3:271598/149‑1 (MQ=255)
                                                                 gTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGcc                                                                                    <  5:143863/149‑1 (MQ=255)
                                                                     ttcGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGa                                                                                 >  1:317046/1‑148 (MQ=255)
                                                                       cgcgCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGa                                                                                 <  1:159100/146‑1 (MQ=255)
                                                                            tCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTcgcg                                                                         >  3:342324/1‑149 (MQ=255)
                                                                              aTGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCgcgc                                                                        <  4:342324/148‑1 (MQ=255)
                                                                              aTGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCa                                                                       <  4:195608/149‑1 (MQ=255)
                                                                                    gCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGt                                                                 >  4:352183/1‑149 (MQ=255)
                                                                                                gATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGa                                                     >  3:24676/1‑149 (MQ=255)
                                                                                                     tGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACg                                                >  7:210843/1‑149 (MQ=255)
                                                                                                      gCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGa                                                     <  4:279002/143‑1 (MQ=255)
                                                                                                       cTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGt                                              <  8:210843/149‑1 (MQ=255)
                                                                                                         cagcagcagCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGa                                            >  6:24230/1‑149 (MQ=255)
                                                                                                             agcagcaCATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCAt                                        <  2:209193/149‑1 (MQ=255)
                                                                                                               cagcaCATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTcgcg                                                                         >  5:320126/1‑114 (MQ=255)
                                                                                                               cagcaCATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTcgcg                                                                         <  6:320126/114‑1 (MQ=255)
                                                                                                                     aTCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGa                                                                                 >  2:148293/1‑100 (MQ=255)
                                                                                                                     aTCTCCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGa                                                                                 <  1:148293/100‑1 (MQ=255)
                                                                                                                        tGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATc                                                       <  4:18344/123‑1 (MQ=255)
                                                                                                                        tGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATc                                                       >  3:18344/1‑123 (MQ=255)
                                                                                                                         gCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGgt                                     <  1:165718/140‑1 (MQ=255)
                                                                                                                         gCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGgt                                     >  2:165718/1‑140 (MQ=255)
                                                                                                                          cGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTcgc                           <  4:24676/149‑1 (MQ=255)
                                                                                                                           gAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTcgcg                          >  5:202480/1‑149 (MQ=255)
                                                                                                                           gAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTcgcg                          >  8:317829/1‑149 (MQ=255)
                                                                                                                            aaCTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCgcgc                         <  2:317046/149‑1 (MQ=255)
                                                                                                                                 gACCTGCTCGATCAGCAAATCTCTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCGGGCTGGCCAGGCTGTCTGCTGCCTGATAGTcgc                                                                          <  5:313966/95‑1 (MQ=255)
                                                                                                                                      gCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGa                                                                                 <  4:273922/83‑1 (MQ=255)
                                                                                                                                      gCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGa                                                                                 >  3:273922/1‑83 (MQ=255)
                                                                                                                                      gCTCGATCAGCAAATCACTGAACGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGa                >  4:286680/1‑148 (MQ=255)
                                                                                                                                            tCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTg         <  5:24230/149‑1 (MQ=255)
                                                                                                                                            tCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTg         <  3:352183/149‑1 (MQ=255)
                                                                                                                                              aGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACCGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGc        >  1:322675/1‑148 (MQ=255)
                                                                                                                                               gCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATc                                                       <  5:284510/100‑1 (MQ=255)
                                                                                                                                               gCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATc                                                       >  6:284510/1‑100 (MQ=255)
                                                                                                                                               gCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGAcc                                          >  3:277528/1‑113 (MQ=255)
                                                                                                                                               gCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGAcc                                          <  4:277528/113‑1 (MQ=255)
                                                                                                                                                 aaaTCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACCGTGACCATGGTGTCCAGTCCCGCCATATCGGACAGACTGCGAcc    >  7:153376/1‑149 (MQ=255)
                                                                                                                                                   aTCACTGATCGACCGCTCGTCTTCTTCGCCAAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGTGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCt  >  3:49346/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CACTTGGCCCATGACCATGGGTACGATCTGCGCTCGGGCATTGAGACTGCGCAAAATAGCGATGAGTTCTTCGCGCTCATGTTGGCTGATCAGGTCGATCTTGCTCAGCAGCAGCACATCTGCGAACTCGACCTGCTCGATCAGCAAATCACTGATCGACCGCTCGTCTTCTTCGCCCAACGTTTCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCT  >  NC_002947/6110859‑6111154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑