Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,143,488	C→A	6.5%	S23S (TCC→TCA)	cusF →	copper efflux protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,143,488	0	C	A	6.5%	190.4 / 3.6	62	S23S (TCC→TCA)	cusF	copper efflux protein
Reads supporting (aligned to +/- strand):  ref base C (33/25);  new base A (2/2);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.49e-01

CGTGATTCCCGCAGCGTATTGGCTGGTCCGACGCCGCGATCTTGTAGTACCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAA  >  NC_002947/6143342‑6143629                                                                                                                                                   |                                                                                                                                              cGTGATTCCCGCAGCGTATTGGCTGGTCCGACGCCGCGATCTTGTAGTACCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCa                                                                                                                                              <  6:163650/148‑1 (MQ=255)        cccGCAGCGTATTGGCTGGTCCGACGCCGCGATCTTGTAGTACCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTcc                                                                                                                                               <  1:192384/140‑1 (MQ=255)        cccGCAGCGTATTGGCTGGTCCGACGCCGCGATCTTGTAGTACCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTcc                                                                                                                                               >  2:192384/1‑140 (MQ=255)        cccGCAGCGTATTGGCTGGTCCGACGCCGCGATCTTGTAGTACCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCgg                                                                                                                                       <  3:225824/148‑1 (MQ=255)         ccGCAGCGTATTGGCTGGTCCGACGCCGCGATCTTGTAGTACCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGa                                                                                                                                     >  5:170517/1‑149 (MQ=255)         ccGCAGCGTATTGGCTGGTCCGACGCCGCGATCTTGTAGTACCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGa                                                                                                                                     >  8:132105/1‑149 (MQ=255)          cGCAGCGTATTTGCTGGTCCGACGCCGCGATCTTGTAGTACCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGAt                                                                                                                                    <  7:317306/149‑1 (MQ=255)          cGCAGCGTATTGGCTGGTCCGACGCCGCGATCTTGTAGTACCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGAt                                                                                                                                    >  6:181892/1‑149 (MQ=255)                  aTTGGCTGGTCCGACGCCGCGATCTTGTAGTACCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCatg                                                                                                                             >  6:40296/1‑148 (MQ=255)                    tGGCTGGTCCGACGCCGCGATCTTGTAGTACCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCTCGCAAGCCCAAGACTCCATGGCCggg                                                                                                                                      <  7:142839/137‑1 (MQ=255)                    tGGCTGGTCCGACGCCGCGATCTTGTAGTACCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCACGCAAGCCCAAGACTCCATGGCCggg                                                                                                                                      >  8:142839/1‑137 (MQ=255)                      gCTGGTCCGACGCCGCGATCTTGTAGTACCTCATAATTCCACCCCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCg                                                                                                                                        <  3:252584/133‑1 (MQ=255)                      gCTGGTCCGACGCCGCGATCTTGTAGTACCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCg                                                                                                                                        >  4:252584/1‑133 (MQ=255)                      gCTGGTCCGACGCCGCGATCTTGTAGTACCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCatggatg                                                                                                                         >  6:299962/1‑148 (MQ=255)                                   cgcgATCTTGTAGTACCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAg                                                                                                           <  7:32118/149‑1 (MQ=255)                                   cgcgATCTTGTAGTACCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAg                                                                                                           >  8:32118/1‑149 (MQ=255)                                   cgcgATCTTGTAGTACCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAg                                                                                                           <  7:132105/149‑1 (MQ=255)                                            gtagtaCCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAAc                                                                                                      <  5:299962/145‑1 (MQ=255)                                             tagtaCCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCatggatg                                                                                                                         <  8:156201/125‑1 (MQ=255)                                             tagtaCCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCatggatg                                                                                                                         >  7:156201/1‑125 (MQ=255)                                              tttaCCTCATAATTCCACACCAGGAGTCATCCGATGAAAAAGCTCTACCTCATCATTGGACTGCTCTTTGCCTTCGCTTCAGGCGGGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGa                                                                                                             <  4:312588/134‑1 (MQ=255)                                              agtaCCTCATAATTACACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGCGATGAGCATGGATGGAATGGatatca                                                                                                             >  3:312588/1‑134 (MQ=255)                                               gtaCCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATc                                                                                                <  5:40296/148‑1 (MQ=255)                                                   cTCATAATTCCACACCAGGAGACATCGGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCAc                                                                                           >  4:86931/1‑149 (MQ=255)                                                   cTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCAc                                                                                           >  8:321247/1‑149 (MQ=255)                                                                acCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGc                                                                              <  1:321347/149‑1 (MQ=255)                                                                    ggAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTcc                                                                                      >  1:53320/1‑137 (MQ=255)                                                                    ggAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTcc                                                                                      <  2:53320/137‑1 (MQ=255)                                                                                tGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAg                                                              >  3:60190/1‑149 (MQ=255)                                                                                tGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAg                                                              <  4:60190/149‑1 (MQ=255)                                                                                      aGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAAtt                                                        <  5:181892/149‑1 (MQ=255)                                                                                             ccTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGATGGTAAAGGCAATTGACGcc                                                  >  6:44765/1‑148 (MQ=255)                                                                                              cTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGccc                                                 >  1:250667/1‑148 (MQ=255)                                                                                              cTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGccc                                                 >  1:359197/1‑148 (MQ=255)                                                                                                  gCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCAAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCGTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAggc                                            >  2:84103/1‑149 (MQ=255)                                                                                                        cACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGcaca                                      <  7:321247/149‑1 (MQ=255)                                                                                                           tGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGt                                    >  1:300455/1‑148 (MQ=255)                                                                                                            gCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGa                                  >  2:179681/1‑149 (MQ=255)                                                                                                             ctctTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAAt                                                                                                 >  8:118469/1‑85 (MQ=255)                                                                                                             ctctTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAAt                                                                                                 <  7:118469/85‑1 (MQ=255)                                                                                                             ctctTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTTACGCCCAAGGCGGCACAGTGAc                                 >  8:293180/1‑149 (MQ=255)                                                                                                             ctctTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGa                                  <  6:170517/148‑1 (MQ=255)                                                                                                                tttGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCt                              >  6:277249/1‑149 (MQ=255)                                                                                                                tttGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCt                              >  5:96857/1‑149 (MQ=255)                                                                                                                tttGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCAATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACgg                                                                   <  7:107445/112‑1 (MQ=255)                                                                                                                tttGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCAATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACgg                                                                   <  7:112390/112‑1 (MQ=255)                                                                                                                tttGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCAATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACgg                                                                   >  8:112390/1‑112 (MQ=255)                                                                                                                tttGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCAATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACgg                                                                   >  8:107445/1‑112 (MQ=255)                                                                                                                 ttGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTACGCTCATGCAGAAGGGAAGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCt                              >  7:271548/1‑148 (MQ=255)                                                                                                                     cTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCAAAGGCGGCACAGTGACCCTGATg                          >  3:267063/1‑148 (MQ=255)                                                                                                                      ttCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCa                        >  5:298636/1‑149 (MQ=255)                                                                                                                                gcgcgcAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCa                                                           <  3:53155/104‑1 (MQ=255)                                                                                                                                gcgcgcAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCa                                                           >  4:53155/1‑104 (MQ=255)                                                                                                                                   cgcAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTtgct           <  2:359197/149‑1 (MQ=255)                                                                                                                                        gCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGtt      <  2:250667/149‑1 (MQ=255)                                                                                                                                         cccAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGATGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTg     <  5:44765/149‑1 (MQ=255)                                                                                                                                          ccAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTg                                   <  7:299644/118‑1 (MQ=255)                                                                                                                                          ccAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTg                                   >  8:299644/1‑118 (MQ=255)                                                                                                                                           cAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGAGCCTGATGCATGGACCGGTTGGGTCGTTGaa   >  8:120007/1‑149 (MQ=255)                                                                                                                                           cAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGa    >  8:203827/1‑148 (MQ=255)                                                                                                                                            aaGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGaaa  >  7:61381/1‑149 (MQ=255)                                                                                                                                            aaGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGaaa  <  8:271548/149‑1 (MQ=255)                                                                                                                                                 tCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGAccc                               <  7:50813/115‑1 (MQ=255)                                                                                                                                                 tCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGAccc                               >  8:50813/1‑115 (MQ=255)                                                                                                                                                   cATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGTCCGGTTGCTGCGtt      <  6:46479/138‑1 (MQ=255)                                                                                                                                                   cATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGTCCGGTTGCTGCGtt      >  5:46479/1‑138 (MQ=255)                                                                                                                                                   |                                                                                                                                              CGTGATTCCCGCAGCGTATTGGCTGGTCCGACGCCGCGATCTTGTAGTACCTCATAATTCCACACCAGGAGACATCCGATGAAAAAGCTCTACCTCAGCATTGCACTGCTCTTTGCCTTCGCATCAGGCGCGCAAGCCCAAGACTCCATGGCCGGGATGAGCATGGATGGAATGGATATGAAGGAAACCCAATCAGCACCTTCCGCTCATGCAGAAGGGACGGTAAAGGCAATTGACGCCCAAGGCGGCACAGTGACCCTGATGCATGGACCGGTTGCTGCGTTGAAA  >  NC_002947/6143342‑6143629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑