Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,172,004 T→G 6.7% N100H (AAC→CAC)  glmS ← L‑glutamine/D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,172,0040TG 6.7% 182.4 / 2.9 60N100H (AAC→CAC) glmSL‑glutamine/D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (25/31);  new base G (2/2);  total (27/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

CCTTAAGGGCGTCGGTCAGGTCTGGAATGCTCTTCAGGGTGTGGTGGATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCTGCCAATGGGTCATCAGCAACAGCAACCTCCAGCTCGCTGAC  >  NC_002947/6171857‑6172148
                                                                                                                                                   |                                                                                                                                                
ccTTAAGGGCGTCGGTCAGGTCTGGAATGCTCTTCAGGGTGTGGTGGATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTtg                                                                                                                                                 >  2:89968/1‑149 (MQ=255)
     aGGGCGTCGGTCAGGTCTGGAATGCTCTTCAGGGTGTGGTGGATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGca                                                                                                                                             <  5:305433/148‑1 (MQ=255)
      gggCGTCGGTCAGGTCTGGAATGCTCTTCAGGGTGTGGTGGATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGGCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGcac                                                                                                                                            >  2:237688/1‑148 (MQ=255)
       ggCGTCGGTCAGGTCTGGAATGCTCTTCAGGGTGTGGTGGATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGcacca                                                                                                                                          <  2:243614/149‑1 (MQ=255)
          gTCGGTCAGGTCTGGAATGCTCTTCAGGGTGTGGTGGATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGc                                                                                                                                              >  1:5574/1‑142 (MQ=255)
          gTCGGTCAGGTCTGGAATGCTCTTCAGGGTGTGGTGGATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGc                                                                                                                                              <  2:5574/142‑1 (MQ=255)
                   ctatctaaTGCTCTTCAGGGTGTGGTGGATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGATCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCAcatca                                                                                                                               <  1:171169/142‑1 (MQ=255)
                    tCTGGAATGCTCTTCAGGCTGTGGTGGATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTAAAGGTTCTCGAAGATGCCGTTGTGCACCACGGCCACATCAtt                                                                                                                             >  5:354247/1‑149 (MQ=255)
                          aTGCTCTTCAGGGTGTGGTGGATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCGTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCga                                                                                                                       <  1:89968/149‑1 (MQ=255)
                          aTGCTCTTCAGGGTGTGGTCGATCAGGTGGACGATGAATTCGGTATAGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTAGCGCAGCTCCTCATGGTTCTCGATCATTCAGTTGTTCACCACGGCCAcatca                                                                                                                               >  2:171169/1‑141 (MQ=255)
                           tGCTCTTCAGGGTGTGGTGGATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCgag                                                                                                                      <  6:136275/149‑1 (MQ=255)
                             ctctTCAGGGTGTGGTGGATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCgaga                                                                                                                     <  4:239573/148‑1 (MQ=255)
                              tctTCAGGGTGTGGTGGATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGACGTTGTGCACCACGGCCACATCATTGCCCGAGAAg                                                                                                                   >  2:93787/1‑149 (MQ=255)
                                ttCAGGGTGTGGTGGATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGtt                                                                                                                                                  >  6:29717/1‑116 (MQ=255)
                                ttCAGGGTGTGGTGGATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGtt                                                                                                                                                  <  5:29717/116‑1 (MQ=255)
                                    gggTGTGGTGGATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGGCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCggg                                                                                                             <  1:237688/149‑1 (MQ=255)
                                     ggTGTGGTGGATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGt                                                                                                            >  5:288470/1‑149 (MQ=255)
                                         tggtggATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCAcatc                                                                                                                                <  8:160941/125‑1 (MQ=255)
                                                  aGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCtt                                                                                                <  6:22077/148‑1 (MQ=255)
                                                   ggTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGTACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCtt                                                                                                >  5:268661/1‑147 (MQ=255)
                                                   ggTGGACGATGACCTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCa                                                                                              <  1:337727/149‑1 (MQ=255)
                                                     tGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGt                                                                                            <  1:92435/149‑1 (MQ=255)
                                                      ggACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTc                                                                                           >  6:10416/1‑149 (MQ=255)
                                                        aCGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCgg                                                                                         <  8:260764/149‑1 (MQ=255)
                                                             gACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGgcgcgc                                                                                    >  3:65110/1‑149 (MQ=255)
                                                              aCTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGc                                                                                                   <  5:193948/133‑1 (MQ=255)
                                                              aCTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGc                                                                                                   >  6:193948/1‑133 (MQ=255)
                                                                ttCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGt                                                                                 >  3:148556/1‑149 (MQ=255)
                                                                  cGGTAGCGGGCTGCGAAGTGAAGACATCGCCCAGGCCCTTCAGCTCTGCGCGCAGCTCCTCATGGTTCTCGTGGATGCCGTTGTGCACCACGGACACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGt                                                                                 <  1:93787/147‑1 (MQ=255)
                                                                         ggTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGcc                                                                         >  6:64245/1‑148 (MQ=255)
                                                                          gTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGcc                                                                         >  2:165927/1‑147 (MQ=255)
                                                                           tCTGAGAAGTGAAGACATATCCCAGGCCCTTCAGCTCTTCGGGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCGCAg                                                                      <  6:354247/149‑1 (MQ=255)
                                                                               cGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCggg                                                                  >  3:202378/1‑149 (MQ=255)
                                                                                    tGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTgg                                                             >  2:252065/1‑149 (MQ=255)
                                                                                     gAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTggg                                                            >  8:48950/1‑149 (MQ=255)
                                                                                      aaGCCATATCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCCGGTGGGCGTTGCCTTCAGTCGGCGCGACGTGAGTAGCCCAGCGCGTGAggg                                                            >  4:175455/1‑148 (MQ=255)
                                                                                      aaGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGcc                                                                         >  8:221092/1‑135 (MQ=255)
                                                                                      aaGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGcc                                                                         <  7:221092/135‑1 (MQ=255)
                                                                                      aaGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTggg                                                            <  4:65110/148‑1 (MQ=255)
                                                                                           aTAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATAc                                                      >  7:33891/1‑149 (MQ=255)
                                                                                                  aGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTg                                               <  1:252065/149‑1 (MQ=255)
                                                                                                    gCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGc                                             >  8:149791/1‑149 (MQ=255)
                                                                                                      ccTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCt                                           >  4:82865/1‑149 (MQ=255)
                                                                                                      ccTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCt                                           <  4:202378/149‑1 (MQ=255)
                                                                                                      ccTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCt                                           <  4:148556/149‑1 (MQ=255)
                                                                                                          cAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCTGc                                         <  5:10416/147‑1 (MQ=255)
                                                                                                                ttCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGTAATACCCAGCTGGCCTGCCAATgg                                   <  7:48950/147‑1 (MQ=255)
                                                                                                                ttCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCTGCCAATgg                                   <  1:165927/147‑1 (MQ=255)
                                                                                                                   gcgcAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCTGCCAATGGGtcat                              <  6:288470/149‑1 (MQ=255)
                                                                                                                   gcgcAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCTGCCAATGGGtcat                              <  5:64245/149‑1 (MQ=255)
                                                                                                                     gcAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAGTACCCAGCTGGCCTGCCAATGGGtcat                              >  5:321849/1‑147 (MQ=255)
                                                                                                                            ctcATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCTGCCAATGGGTCATcagcaacag                     >  1:191285/1‑149 (MQ=255)
                                                                                                                            ctcATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCTGCCAATGGGTCATcagc                          >  6:42399/1‑144 (MQ=255)
                                                                                                                            ctcATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCTGCCAATGGGTCATcagc                          <  5:42399/144‑1 (MQ=255)
                                                                                                                              cATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCTGCCAATGGGTCTTcagcaacagc                    >  3:7540/1‑148 (MQ=255)
                                                                                                                                tGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGATAAGTGCGGGTGGGGGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGTGTGTGGGCAATACCCAGCTGGCCTGCCAATGGGTCATCagcaacagcaac                 <  3:175455/149‑1 (MQ=255)
                                                                                                                                   ttCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCTGCCAATGGGTCATCAGCAACAGCAACCTc              <  8:33891/149‑1 (MQ=255)
                                                                                                                                         atgtggCCGTGGTGCACAACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCTGCCAATGGGTCATCAGCAACAGCAACCTc              <  2:304266/138‑1 (MQ=255)
                                                                                                                                         atgtggCCGTGGTGCACAACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCTGCCAATGGGTCATCAGCAACAGCAACCTc              >  1:330763/6‑143 (MQ=255)
                                                                                                                                         atgtggCCGTGGTGCACAACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCTGCCAATGGGTCATCAGCAACAGCAACCTc              >  1:304266/6‑143 (MQ=255)
                                                                                                                                         atgtggCCGTGGTGCACAACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCTGCCAATGGGTCATCAGCAACAGCAACCTc              <  2:330763/138‑1 (MQ=255)
                                                                                                                                             tgCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCTGCCAATGGGTCATCAGCAACAGCAACCTCCAGCTCGCTg    <  7:149791/149‑1 (MQ=255)
                                                                                                                                               ccGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCTGCCAATGGGTCATCAGCAACAGCAACCTCCAGCTCGCTGAc  <  6:321849/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CCTTAAGGGCGTCGGTCAGGTCTGGAATGCTCTTCAGGGTGTGGTGGATCAGGTGGACGATGACTTCGGTATCGGTCTGCGAAGTGAAGACATAGCCCAGGCCCTTCAGCTCTTCGCGCAGCTCCTCATGGTTCTCGATGATGCCGTTGTGCACCACGGCCACATCATTGCCCGAGAAGTGCGGGTGGGCGTTGCCTTCAGTCGGCGCGCCGTGAGTAGCCCAGCGGGTGTGGGCAATACCCAGCTGGCCTGCCAATGGGTCATCAGCAACAGCAACCTCCAGCTCGCTGAC  >  NC_002947/6171857‑6172148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑