Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 435,369 T→C 6.8% intergenic (‑170/‑280) glcB ← / → PP_0357 malate synthase G/ACT domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947435,3690TC 6.8% 170.8 / 2.5 61intergenic (‑170/‑280)glcB/PP_0357malate synthase G/ACT domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (26/30);  new base C (2/2);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

CGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTCGCGTATGGTCACGCTGGGAGTAGGTATGTTCGCAAAAAACAGGTGGATTGTT  >  NC_002947/435227‑435517
                                                                                                                                              |                                                                                                                                                    
cGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCaaa                                                                                                                                                 <  2:209614/148‑1 (MQ=255)
 gCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAac                                                                                                                                                <  4:168511/148‑1 (MQ=255)
     ttctGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGt                                                                                                                                           >  3:223551/1‑149 (MQ=255)
     ttctGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACACCCAAACGACGt                                                                                                                                           >  3:133687/1‑149 (MQ=255)
     ttctGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACACCCAAACGACGt                                                                                                                                           >  3:128527/1‑149 (MQ=255)
       ctGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGttt                                                                                                                                         >  6:8173/1‑149 (MQ=255)
          tATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAGACGACGTTTTg                                                                                                                                       >  3:79846/1‑148 (MQ=255)
          tATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTg                                                                                                                                       >  3:128911/1‑148 (MQ=255)
            ttAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGTGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTa                                                                                                                                     <  7:78961/148‑1 (MQ=255)
            ttAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTa                                                                                                                                     <  5:167982/148‑1 (MQ=255)
            ttAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACACCCAAACGACGTTTTGTa                                                                                                                                     <  4:133687/148‑1 (MQ=255)
            ttAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACACCCAAACGACGTTTTGTa                                                                                                                                     <  4:128527/148‑1 (MQ=255)
                gCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAac                                                                                                                                                >  2:16321/1‑133 (MQ=255)
                gCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAac                                                                                                                                                <  1:16321/133‑1 (MQ=255)
                     tAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCa                                                                                                                           >  2:258343/1‑149 (MQ=255)
                        cgcTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACgg                                                                                                                        <  4:223551/149‑1 (MQ=255)
                        cgcTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACgg                                                                                                                        <  5:8173/149‑1 (MQ=255)
                        cgcTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACgg                                                                                                                        <  6:151048/149‑1 (MQ=255)
                             ctctAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGAta                                                                                                                    <  3:81898/148‑1 (MQ=255)
                                tAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGAtacta                                                                                                                 >  5:236446/1‑148 (MQ=255)
                                  aaCACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGAtactac                                                                                                                <  5:104588/147‑1 (MQ=255)
                                     acGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGaa                                                                                                           >  5:360884/1‑149 (MQ=255)
                                      cGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGAtac                                                                                                                   <  1:159872/140‑1 (MQ=255)
                                      cGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGAtac                                                                                                                   >  2:159872/1‑140 (MQ=255)
                                           ccAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAg                                                                                                       >  2:227397/1‑147 (MQ=255)
                                           ccAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAg                                                                                                       >  2:27834/1‑147 (MQ=255)
                                            cAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGtttt                                                                                                                                        >  4:100496/1‑113 (MQ=255)
                                            cAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGtttt                                                                                                                                        <  3:100496/113‑1 (MQ=255)
                                              gTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGc                                                                                                  >  3:8215/1‑149 (MQ=255)
                                                 cATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCgg                                                                                                <  4:128911/148‑1 (MQ=255)
                                                      ccccGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCaa                                                                                           >  8:281291/1‑148 (MQ=255)
                                                       cccGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCaaaa                                                                                         <  1:258343/149‑1 (MQ=255)
                                                            ggTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGa                                                                                    <  4:323016/149‑1 (MQ=255)
                                                                  ttGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTaa                                                                                >  5:259096/1‑147 (MQ=255)
                                                                    gggggCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAg                                                                             <  3:124945/148‑1 (MQ=255)
                                                                     ggggCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAgcg                                                                           >  6:152715/1‑149 (MQ=255)
                                                                       ggcggcTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTAAATTAAGCAGTAGGCGGGGCAAAATCAGCCTAAAAgcg                                                                           >  4:252836/1‑147 (MQ=255)
                                                                       ggcggcTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGGGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGGCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAgcg                                                                           <  3:252836/147‑1 (MQ=255)
                                                                        gcggcTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCg                                                                        >  2:150384/1‑149 (MQ=255)
                                                                        gcggcTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCg                                                                        <  6:360884/149‑1 (MQ=255)
                                                                        gcggcTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCg                                                                        >  1:227635/1‑149 (MQ=255)
                                                                           gcTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACAGGAAGCAGTAGGCGGGCCAAAATCAGACTAAAAGCGCCGTTa                                                                     >  7:96320/1‑149 (MQ=255)
                                                                             tGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATg                                                                   >  7:265966/1‑149 (MQ=255)
                                                                               ggTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGc                                                                  <  1:150384/148‑1 (MQ=255)
                                                                                gTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGa                                                                <  1:332671/149‑1 (MQ=255)
                                                                                    ggCGTGCCAGACATGCTGGCGGGCAGGGTGCTTCTTGTCCCCGGCGCCGTGGCAGACAACCATACGACCTTTTGCAGTCCGCGCCACGGTATACTACATGAACCAGTAGCGGGGGCAAAATCAGACTAAACGCGCGCTTATCCCACCCg                                                            <  8:96320/149‑1 (MQ=255)
                                                                                    ggCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCa                                                                                      >  5:1911/1‑123 (MQ=255)
                                                                                    ggCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCa                                                                                      <  6:1911/123‑1 (MQ=255)
                                                                                       cTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTg                                                         <  2:338877/149‑1 (MQ=255)
                                                                                          ccAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTc                                                      <  5:12336/149‑1 (MQ=255)
                                                                                            aGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAGACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTcgc                                                    <  4:79846/149‑1 (MQ=255)
                                                                                             gACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTcgcg                                                   >  6:235317/1‑149 (MQ=255)
                                                                                                  gCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTCGCGTATgg                                              <  6:236446/149‑1 (MQ=255)
                                                                                                  gCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTCGCGTATgg                                              <  1:227397/149‑1 (MQ=255)
                                                                                                  gCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTCGCGTATgg                                              <  7:281291/149‑1 (MQ=255)
                                                                                                           cAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTCGCGTATGGTCACGCTgg                                     <  5:152715/149‑1 (MQ=255)
                                                                                                            aGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTCGCGTATGGTCACGCTggg                                    <  2:227635/149‑1 (MQ=255)
                                                                                                                           acaGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGACGATATCCGACCCGTTGGTCGCGTAAGGTCACGCTGGGAGAAGGTATGTTCg                      >  6:31877/1‑148 (MQ=255)
                                                                                                                                        cAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTCGCGTATGGTCACGCTGGGAGTAGGTATGTTCGCAAAAAACAGGTg         >  3:241606/1‑148 (MQ=255)
                                                                                                                                         aGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTCGCGTATGGTCACGCTGGGAGTAGGTATGTTc                       <  4:206282/133‑1 (MQ=255)
                                                                                                                                         aGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTCGCGTATGGTCACGCTGGGAGTAGGTATGTTc                       >  3:206282/1‑133 (MQ=255)
                                                                                                                                              tCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTCGCGTATGGTCACGCTGGGAGTAGGTATGTTCGCAAAAAACAGGTGGATTGtt  <  8:265966/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CGCTCTTCTGTATTAAGCCTGTAGCGCTTCTCTAAACACGGCACCAGTGCATGCCCCCGGGGTTGCTTGGGGGCGGCTGGGTGCGGCCTGCCAGACAGGCTGGCGGGCAGTGTGCGTATTTTCACAGGCGCCTTGGCAGACATCCAAACGACGTTTTGTAGTCCGCGCCACGGGATACTACATGAAGCAGTAGGCGGGGCAAAATCAGACTAAAAGCGCCGTTATGCGACCCGTTGGTCGCGTATGGTCACGCTGGGAGTAGGTATGTTCGCAAAAAACAGGTGGATTGTT  >  NC_002947/435227‑435517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑