Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	465,442	A→G	11.9%	L124L (TTG→CTG)	PP_0384 ←	AsnC family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	465,442	0	A	G	11.9%	146.1 / 10.9	59	L124L (TTG→CTG)	PP_0384	AsnC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (28/24);  new base G (4/3);  total (32/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01

CCCGCTCCTACAACCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATCCC  >  NC_002947/465299‑465586                                                                                                                                                |                                                                                                                                                  cccGCTCCTACAACCGGCCTCATCGATACCCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCCCGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGAt                                                                                                                                              >  5:133213/1‑149 (MQ=255) cccGCTCCTACAACCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGCTTTTATCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGAt                                                                                                                                              >  1:118904/1‑149 (MQ=255)   cGCTCCTACAACCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATcc                                                                                                                                            <  4:293000/149‑1 (MQ=255)             aCCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCgg                                                                                                                                  <  4:13274/149‑1 (MQ=255)               cGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGc                                                                                                                                <  4:273854/149‑1 (MQ=255)                      aTCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTcc                                                                                                                          <  4:147397/148‑1 (MQ=255)                      aTCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCg                                                                                                                         >  7:289782/1‑149 (MQ=255)                          aTAACCCCACCCGAGTGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCCCCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGGGCAACTGCTCCAGCTGCTCGGGCGAGTCCGGACg                                                                                                                     >  2:119280/1‑149 (MQ=255)                          aTAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAGCTGGATCAGTTGCTCgac                                                                                                                                 <  7:41067/137‑3 (MQ=255)                          aTAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAGCTGGATCAGTTGCTCgac                                                                                                                                 >  8:46791/1‑135 (MQ=255)                          aTAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAGCTGGATCAGTTGCTCgac                                                                                                                                 >  8:41067/1‑135 (MQ=255)                          aTAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAGCTGGATCAGTTGCTCgac                                                                                                                                 <  7:46791/137‑3 (MQ=255)                              ccccACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGc                                                                                                                 >  8:40050/1‑149 (MQ=255)                               cccACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAGCTGGATCAGTTGCTCga                                                                                                                                  >  7:30891/1‑130 (MQ=255)                               cccACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAGCTGGATCAGTTGCTCga                                                                                                                                  <  8:30891/131‑2 (MQ=255)                               cccACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGc                                                                                                                 >  2:278356/1‑148 (MQ=255)                                      gAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGAc                                                                                                        <  8:289782/149‑1 (MQ=255)                                           ccAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGt                                                                                                   >  1:322081/1‑149 (MQ=255)                                            cAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGATGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTc                                                                                                  <  4:193919/149‑1 (MQ=255)                                              gCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCg                                                                                                 >  3:319403/1‑148 (MQ=255)                                                ccGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAAt                                                                                              <  7:40050/149‑1 (MQ=255)                                                  gTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACa                                                                                                       >  7:64938/1‑138 (MQ=255)                                                  gTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACa                                                                                                       <  8:64938/138‑1 (MQ=255)                                                  gTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTc                                                                                            <  1:278356/149‑1 (MQ=255)                                                  gTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTc                                                                                            >  1:250189/1‑149 (MQ=255)                                                              tGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCg                                                                                 >  5:123176/1‑148 (MQ=255)                                                                  cACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGcaca                                                                             >  6:275526/1‑148 (MQ=255)                                                                  cACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGcaca                                                                             >  6:79566/1‑148 (MQ=255)                                                                    cGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAg                                                                          >  1:131622/1‑149 (MQ=255)                                                                         cACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACa                                                                                                       <  5:12574/115‑1 (MQ=255)                                                                         cACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACa                                                                                                       >  6:12574/1‑115 (MQ=255)                                                                           ccTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGAGCGACATAGTCGAATTCGCCGCTTACCGca                                                                               <  1:213776/138‑1 (MQ=255)                                                                           ccTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGca                                                                               >  2:213776/1‑137 (MQ=255)                                                                                ctgctgATCATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGACTTCGCCGCTTCCCGCACACAGCTGCTGCACCt                                                               <  6:301967/148‑1 (MQ=255)                                                                                  gctgAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGc                                                            >  6:182098/1‑149 (MQ=255)                                                                                   ctgAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGc                                                            <  5:275526/148‑1 (MQ=255)                                                                                      agaATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGccc                                                          <  5:182098/147‑1 (MQ=255)                                                                                            aTCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACt                                                  >  1:72847/1‑149 (MQ=255)                                                                                                aCGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAg                                                                          >  4:313149/1‑121 (MQ=255)                                                                                                aCGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAg                                                                          <  3:313149/121‑1 (MQ=255)                                                                                                             ctcCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGt                                 >  2:12990/1‑149 (MQ=255)                                                                                                                cACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTcc                               <  2:322081/148‑1 (MQ=255)                                                                                                                cACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCt                              >  2:24978/1‑149 (MQ=255)                                                                                                                cACCCCGTCGACACTGCCAATCGGGTCGAGCAACTGATACAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGcaca                                                                             >  7:54587/1‑102 (MQ=255)                                                                                                                cACCCCCTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGcaca                                                                             <  8:54587/102‑1 (MQ=255)                                                                                                                 aCCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGcaccac                                   >  7:332043/1‑143 (MQ=255)                                                                                                                 aCCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGcaccac                                   <  8:332043/143‑1 (MQ=255)                                                                                                                   cccGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGc                           <  8:221012/149‑1 (MQ=255)                                                                                                                    ccGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGcc                          >  6:285782/1‑149 (MQ=255)                                                                                                                          acacTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCg                    >  2:77010/1‑149 (MQ=255)                                                                                                                            acTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTg                  <  4:343666/149‑1 (MQ=255)                                                                                                                              tcataaTCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGc                <  8:165389/145‑1 (MQ=255)                                                                                                                              tGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGc                >  8:85781/1‑149 (MQ=255)                                                                                                                                  aaTCTGGTCGAGCAACTGATCGAGCTGCTCGGGGGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGc             <  6:85232/148‑1 (MQ=255)                                                                                                                                  aaTCAGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAAATCGCCGCTTACCGCACACAGCTGCAGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGAGGCTGc             >  5:85232/1‑148 (MQ=255)                                                                                                                                       gcccGAGCAGCTGGATCAGTTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCtt        >  6:255207/4‑148 (MQ=255)                                                                                                                                        gTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCtt        <  2:118904/147‑1 (MQ=255)                                                                                                                                        gTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCtt        <  1:12990/147‑1 (MQ=255)                                                                                                                                            aGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATccc  >  1:108318/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                  CCCGCTCCTACAACCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATCCC  >  NC_002947/465299‑465586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑