Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	465,452	C→T	11.3%	Q120Q (CAG→CAA)	PP_0384 ←	AsnC family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	465,452	0	C	T	11.3%	163.0 / 10.1	62	Q120Q (CAG→CAA)	PP_0384	AsnC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (26/29);  new base T (4/3);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

ACCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATCCCGACATACGCTT  >  NC_002947/465311‑465597                                                                                                                                              |                                                                                                                                                   aCCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCgg                                                                                                                                             <  4:13274/149‑1 (MQ=255)   cGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGc                                                                                                                                           <  4:273854/149‑1 (MQ=255)          aTCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTcc                                                                                                                                     <  4:147397/148‑1 (MQ=255)          aTCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCg                                                                                                                                    >  7:289782/1‑149 (MQ=255)              aTAACCCCACCCGAGTGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCCCCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGGGCAACTGCTCCAGCTGCTCGGGCGAGTCCGGACg                                                                                                                                >  2:119280/1‑149 (MQ=255)              aTAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAGCTGGATCAGTTGCTCgac                                                                                                                                            >  8:41067/1‑135 (MQ=255)              aTAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAGCTGGATCAGTTGCTCgac                                                                                                                                            >  8:46791/1‑135 (MQ=255)              aTAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAGCTGGATCAGTTGCTCgac                                                                                                                                            <  7:41067/137‑3 (MQ=255)              aTAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAGCTGGATCAGTTGCTCgac                                                                                                                                            <  7:46791/137‑3 (MQ=255)                  ccccACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGc                                                                                                                            >  8:40050/1‑149 (MQ=255)                   cccACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAGCTGGATCAGTTGCTCga                                                                                                                                             >  7:30891/1‑130 (MQ=255)                   cccACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAGCTGGATCAGTTGCTCga                                                                                                                                             <  8:30891/131‑2 (MQ=255)                   cccACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGc                                                                                                                            >  2:278356/1‑148 (MQ=255)                          gAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGAc                                                                                                                   <  8:289782/149‑1 (MQ=255)                               ccAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGt                                                                                                              >  1:322081/1‑149 (MQ=255)                                cAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGATGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTc                                                                                                             <  4:193919/149‑1 (MQ=255)                                  gCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCg                                                                                                            >  3:319403/1‑148 (MQ=255)                                    ccGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAAt                                                                                                         <  7:40050/149‑1 (MQ=255)                                      gTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACa                                                                                                                  <  8:64938/138‑1 (MQ=255)                                      gTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACa                                                                                                                  >  7:64938/1‑138 (MQ=255)                                      gTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTc                                                                                                       <  1:278356/149‑1 (MQ=255)                                      gTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTc                                                                                                       >  1:250189/1‑149 (MQ=255)                                                  tGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCg                                                                                            >  5:123176/1‑148 (MQ=255)                                                      cACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGcaca                                                                                        >  6:79566/1‑148 (MQ=255)                                                      cACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGcaca                                                                                        >  6:275526/1‑148 (MQ=255)                                                        cGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAg                                                                                     >  1:131622/1‑149 (MQ=255)                                                             cACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACa                                                                                                                  <  5:12574/115‑1 (MQ=255)                                                             cACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACa                                                                                                                  >  6:12574/1‑115 (MQ=255)                                                               ccTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAGAGCGACATAGTCGAATTCGCCGCTTACCGca                                                                                          <  1:213776/138‑1 (MQ=255)                                                               ccTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGca                                                                                          >  2:213776/1‑137 (MQ=255)                                                                    ctgctgATCATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGACTTCGCCGCTTCCCGCACACAGCTGCTGCACCt                                                                          <  6:301967/148‑1 (MQ=255)                                                                      gctgAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGc                                                                       >  6:182098/1‑149 (MQ=255)                                                                       ctgAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGc                                                                       <  5:275526/148‑1 (MQ=255)                                                                          agaATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGccc                                                                     <  5:182098/147‑1 (MQ=255)                                                                                aTCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACt                                                             >  1:72847/1‑149 (MQ=255)                                                                                    aCGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAg                                                                                     >  4:313149/1‑121 (MQ=255)                                                                                    aCGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAg                                                                                     <  3:313149/121‑1 (MQ=255)                                                                                                 ctcCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGt                                            >  2:12990/1‑149 (MQ=255)                                                                                                    cACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTcc                                          <  2:322081/148‑1 (MQ=255)                                                                                                    cACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCt                                         >  2:24978/1‑149 (MQ=255)                                                                                                    cACCCCGTCGACACTGCCAATCGGGTCGAGCAACTGATACAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGcaca                                                                                        >  7:54587/1‑102 (MQ=255)                                                                                                    cACCCCCTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGcaca                                                                                        <  8:54587/102‑1 (MQ=255)                                                                                                     aCCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGcaccac                                              >  7:332043/1‑143 (MQ=255)                                                                                                     aCCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGcaccac                                              <  8:332043/143‑1 (MQ=255)                                                                                                       cccGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGc                                      <  8:221012/149‑1 (MQ=255)                                                                                                        ccGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGcc                                     >  6:285782/1‑149 (MQ=255)                                                                                                              acacTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCg                               >  2:77010/1‑149 (MQ=255)                                                                                                                acTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTg                             <  4:343666/149‑1 (MQ=255)                                                                                                                  tcataaTCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGc                           <  8:165389/145‑1 (MQ=255)                                                                                                                  tGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGc                           >  8:85781/1‑149 (MQ=255)                                                                                                                      aaTCTGGTCGAGCAACTGATCGAGCTGCTCGGGGGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGc                        <  6:85232/148‑1 (MQ=255)                                                                                                                      aaTCAGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAAATCGCCGCTTACCGCACACAGCTGCAGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGAGGCTGc                        >  5:85232/1‑148 (MQ=255)                                                                                                                           gcccGAGCAGCTGGATCAGTTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCtt                   >  6:255207/4‑148 (MQ=255)                                                                                                                            gTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCtt                   <  1:12990/147‑1 (MQ=255)                                                                                                                            gTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCtt                   <  2:118904/147‑1 (MQ=255)                                                                                                                                aGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATccc             >  1:108318/1‑149 (MQ=255)                                                                                                                                     cTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATCCCGACAt        <  5:285782/149‑1 (MQ=255)                                                                                                                                     cTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATCCCGACAt        <  1:77010/149‑1 (MQ=255)                                                                                                                                        aTCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGcaca                                                                                        <  8:242231/66‑1 (MQ=255)                                                                                                                                           cAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATCCCGACATACGCtt  <  6:123176/149‑1 (MQ=255)                                                                                                                                           cAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATCCCGACATACGCtt  <  5:79566/149‑1 (MQ=255)                                                                                                                                           cAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATCCCGACATACGCtt  <  2:131622/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                   ACCGGCCTCATCGATAACCCCACCCGAGCGCCAGCCCGTTCAAAGCGGCTGTCCACGGTCCACCTTGCTGCTGAGAATGATCGACGTGGTGGTTTTCTCCACCCCGTCGACACTGCCAATCTGGTCGAGCAACTGATCCAGCTGCTCGGGCGAGTCCGTACGCAGCCAG‑GCGACATAGTCGAATTCGCCGCTTACCGCACACAGCTGCTGCACCTGGCCCATGGCACTCAGGCGCCGCACCACGTCCTTGCCGGAGCGTGGCTGCACCTTGATCCCGACATACGCTT  >  NC_002947/465311‑465597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑