Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	473,169	A→G	6.2%	F606L (TTT→CTT)	dnaG ←	DNA primase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	473,169	0	A	G	6.2%	187.0 / 2.1	64	F606L (TTT→CTT)	dnaG	DNA primase
Reads supporting (aligned to +/- strand):  ref base A (32/28);  new base G (2/2);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

CGTTTGTGCGGGAACATTTCGGCTCAGCAGGGCGAGGAGCTGGGTTTTTTCCTCGCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGC  >  NC_002947/473023‑473313                                                                                                                                                   |                                                                                                                                                 cGTTTGTGCGGGAACATTTCGGCTCAGCAGGGCGAGGAGCTGGGTTTTTTCCTCGCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACt                                                                                                                                                <  8:62620/149‑1 (MQ=255)      gtgCGGGAACATTTCGGCTCAGCAGGGCGAGGAGCTGGGTTTTTTCCTCGCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACtgttg                                                                                                                                            <  7:295227/148‑1 (MQ=255)           ggAACATTTCGGCTCAGCAGGGCGAGGAGCTGGGTTTTTTCCTCGCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAg                                                                                                                                      >  8:1309/1‑149 (MQ=255)            gcaCATTTCGGCGCAGCACGGCGAGGAGCTGGGTTTTTTCCTCGCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAgg                                                                                                                                     <  6:303787/147‑1 (MQ=255)                  ttCGGCTCAGCAGGGCGAGGAGCTGGGTTTTTTCCTCGCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGAAAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCg                                                                                                                               >  6:353645/1‑149 (MQ=255)                   tCGGCTCAGCAGGGCGAGGAGCTGGGTTTTTTCCTCGCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCg                                                                                                                               <  2:12784/148‑1 (MQ=255)                          agcagGGCGAGGAGCTGGGTTTTTTCCTCGCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGa                                                                                                                        >  1:100880/1‑148 (MQ=255)                                         tGGGTTTTTTCCTCGCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTc                                                                                                        >  6:162772/1‑149 (MQ=255)                                          gggTTTTTTCCTCGCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCAtt                                                                                                               <  2:321234/141‑1 (MQ=255)                                          gggTTTTTTCCTCGCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCAtt                                                                                                               >  1:321234/1‑141 (MQ=255)                                             ttttttCCTCGCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGc                                                                                                     >  3:118798/1‑148 (MQ=255)                                                 ttCCTCGCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAggg                                                                                                >  3:347342/1‑149 (MQ=255)                                                 ttCCTCGCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAggg                                                                                                >  4:214906/1‑149 (MQ=255)                                                     tCGCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGcgcg                                                                                            >  8:68433/1‑149 (MQ=255)                                                      cGCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGcgcg                                                                                            >  5:34459/1‑148 (MQ=255)                                                       gCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGcgcg                                                                                            >  5:30832/1‑147 (MQ=255)                                                       gCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGt                                                                                           <  4:347342/148‑1 (MQ=255)                                                         tGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAg                                                                                         >  7:176090/1‑148 (MQ=255)                                                              aaTTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAggcg                                                                                   >  3:89724/1‑149 (MQ=255)                                                              aaTTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAggcg                                                                                   >  3:312760/1‑149 (MQ=255)                                                               aTTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAggcgg                                                                                  <  4:118798/149‑1 (MQ=255)                                                               aTTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAggcgg                                                                                  <  4:312760/149‑1 (MQ=255)                                                                ttCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAggcggc                                                                                 >  3:70153/1‑149 (MQ=255)                                                                  cGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGcc                                                                                <  2:100880/148‑1 (MQ=255)                                                                   gCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCt                                                                              <  6:34459/149‑1 (MQ=255)                                                                     tttGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAggcgg                                                                                  <  5:284604/143‑1 (MQ=255)                                                                     tttGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAggcgg                                                                                  >  6:284604/1‑143 (MQ=255)                                                                      ttGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGtt                                                                           >  4:46003/1‑149 (MQ=255)                                                                        gACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCg                                                                         >  7:264294/1‑149 (MQ=255)                                                                          cGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGt                                                                       <  3:214906/149‑1 (MQ=255)                                                                                ttCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGtgccatg                                                                 >  5:56825/1‑149 (MQ=255)                                                                                   cTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGtgccatgcca                                                              <  5:162772/149‑1 (MQ=255)                                                                                   cTGAGCAGTTGCTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAGCTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGtgccatgcc                                                               >  1:24907/1‑148 (MQ=255)                                                                                   cTGAGCAGTTGCTCCAGGCTGCGCTCGCGTNGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAGCTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGtgccatgcc                                                               >  1:30382/1‑148 (MQ=255)                                                                                    tGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCg                                                                         <  3:60440/137‑1 (MQ=255)                                                                                    tGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCg                                                                         >  4:60440/1‑137 (MQ=255)                                                                                      aGCAGTTGCTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAGCTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAAc                                                            <  2:30382/148‑1 (MQ=255)                                                                                      aGCAGTTGCTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAGCTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAAc                                                            <  2:24907/148‑1 (MQ=255)                                                                                           ttgttCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGa                                                      <  5:353645/149‑1 (MQ=255)                                                                                              ttCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATc                                                    >  1:76205/1‑148 (MQ=255)                                                                                                 cAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTg                                                         >  2:134011/1‑140 (MQ=255)                                                                                                 cAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTg                                                         <  1:134011/140‑1 (MQ=255)                                                                                                      tGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCGtt                                           >  1:241395/1‑149 (MQ=255)                                                                                                       gcgcTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTg                                          <  7:1309/149‑1 (MQ=255)                                                                                                         gcTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAg                                        <  2:158823/149‑1 (MQ=255)                                                                                                         gcTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAg                                        >  1:158823/1‑149 (MQ=255)                                                                                                           tCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCAt                                            >  6:184466/1‑143 (MQ=255)                                                                                                           tCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCAt                                            <  5:184466/143‑1 (MQ=255)                                                                                                           tCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCt                                      >  7:77964/1‑149 (MQ=255)                                                                                                                ttGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGcc                                 <  7:68433/149‑1 (MQ=255)                                                                                                                   gcgggcggACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCa                               <  4:89724/148‑1 (MQ=255)                                                                                                                   gcgggcggACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAg                              >  2:166016/1‑149 (MQ=255)                                                                                                                          gACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGAttttt                       <  8:264294/149‑1 (MQ=255)                                                                                                                           aCAAGCTAGTTATAGTGTCGAAAAACTGTAGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGAACGGTGCCATGCCAAc                                                            >  6:164759/1‑111 (MQ=255)                                                                                                                             aaGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTg                    <  4:70153/149‑1 (MQ=255)                                                                                                                                cTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAg                                        <  7:71223/126‑1 (MQ=255)                                                                                                                                cTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAg                                        >  8:71223/1‑126 (MQ=255)                                                                                                                                cTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCa                               >  6:275055/1‑135 (MQ=255)                                                                                                                                cTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCa                               <  5:275055/135‑1 (MQ=255)                                                                                                                                cTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTgcgc                 <  8:176090/149‑1 (MQ=255)                                                                                                                                   gTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCt                                      >  6:254855/1‑125 (MQ=255)                                                                                                                                   gTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCt                                      <  5:254855/125‑1 (MQ=255)                                                                                                                                          tgtCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGAt        >  1:140946/1‑148 (MQ=255)                                                                                                                                            tCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCaa     <  3:118345/149‑1 (MQ=255)                                                                                                                                               aaaaaCTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGc  >  3:342777/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                 CGTTTGTGCGGGAACATTTCGGCTCAGCAGGGCGAGGAGCTGGGTTTTTTCCTCGCTGGTCAATTCGCTTTGACGTGATTTCCTGAGCAGTTGTTCCAGGCTGCGCTCGCGTTGGCGGGCGGACAAGCTAGTTATAGTGTCGAAAAACTGTTGTTCAAGGTTGTCGGCCACGATAAGCCATTCCTTTTCCGCCAGGGCGCGTAGCAGGCGGCCCTGTTCGGTGCCATGCCAACGTGCGATCAACTGCATTGAGCTTAGCCCAGGATTTTTCTGCGCGGCTTCGATCAATGC  >  NC_002947/473023‑473313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑