Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 491,016 T→C 6.5% T469A (ACC→GCC)  lptD ← LPS‑assembly protein LptD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947491,0160TC 6.5% 221.2 / 3.7 76T469A (ACC→GCC) lptDLPS‑assembly protein LptD
Reads supporting (aligned to +/- strand):  ref base T (31/40);  new base C (3/2);  total (34/42)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCAAAGACGGCACTGTCCTTCAGATCACGATCAAAGCGAACAGCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCTTTCCACGCCAATCTGGTCGGATTCCAGGTCCTGGAAGTAGAACGGGTCGCTGATGTCG  >  NC_002947/490870‑491164
                                                                                                                                                    |                                                                                                                                                    
atcaAAGACGGCACTGTCCTTCAGATCACGATCAAAGCGAACAGCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTc                                                                                                                                                     <  7:338192/148‑1 (MQ=255)
 tcaAAGACGGCACTGTCCTTCAGATCACGATCAAAGCGAACAGCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGc                                                                                                                                                   >  1:179588/1‑149 (MQ=255)
       aCGGCACTGTCCTTCAGATCACGATCAAAGCGAACAGCTTCAGTCTCGTGACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCAtct                                                                                                                                              >  1:84636/1‑149 (MQ=255)
           cACTGTCCTTCAGATCACGATCAAAGCGAACAGCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAg                                                                                                                                         <  3:313550/149‑1 (MQ=255)
            aCTGTCCTTCAGATCACGATCAAAGCGAACAGCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAgg                                                                                                                                        >  6:182790/1‑149 (MQ=255)
                tCCTTCAGATCACGATCAAAGCGAACAGCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTACGAGATGGCGACCATCTCGCAgatca                                                                                                                                     <  4:93101/148‑5 (MQ=255)
                    tCAGATCACGATCAAAGCGAACAGCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGcaca                                                                                                                                >  4:95178/1‑149 (MQ=255)
                    tCAGATCACGATCAAAGCGAACAGCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGcaca                                                                                                                                >  4:333272/1‑149 (MQ=255)
                     cAGATCACGATCAAAGCGAACAGCTTCAGTCTCGT‑ACCCAACGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGcac                                                                                                                                 <  6:178463/147‑1 (MQ=255)
                       gATCACGATCAAAGCGAACAGCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTc                                                                                                                             <  7:32563/149‑1 (MQ=255)
                         tCACGATCAAAGCGAACAGCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCaa                                                                                                                           >  4:344971/1‑149 (MQ=255)
                         tCACGATCAAAGCGAACAGCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCaa                                                                                                                           >  2:109564/1‑149 (MQ=255)
                         tCACGATCAAAGCGAACAGCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCaa                                                                                                                           <  3:333272/149‑1 (MQ=255)
                          cACGATCAAAGCGAACAGCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGcc                                                                                                                                                  >  8:179644/1‑125 (MQ=255)
                          cACGATCAAAGCGAACAGCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGcc                                                                                                                                                  <  7:179644/125‑1 (MQ=255)
                                  aaGCGAACAGCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGgt                                                                                                                  >  1:56326/1‑149 (MQ=255)
                                    gCGAACAGCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGgtgt                                                                                                                <  2:56326/149‑1 (MQ=255)
                                     cGAACAGCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTa                                                                                                               <  7:109783/149‑1 (MQ=255)
                                           gCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTc                                                                                                         <  2:84636/149‑1 (MQ=255)
                                            cTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCg                                                                                                        >  4:78097/1‑149 (MQ=255)
                                            cTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCg                                                                                                        >  4:177942/1‑149 (MQ=255)
                                                 gTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCg                                                                                                   <  6:105766/149‑1 (MQ=255)
                                                    tcGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTc                                                                                                                                                     >  8:261529/1‑96 (MQ=255)
                                                    tcGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTc                                                                                                                                                     <  7:261529/96‑1 (MQ=255)
                                                    tcGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGCCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCgg                                                                                                  >  3:193545/1‑147 (MQ=255)
                                                      gT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAg                                                                                               <  3:177942/148‑1 (MQ=255)
                                                      gT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAg                                                                                               <  2:179588/148‑1 (MQ=255)
                                                       t‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGt                                                                                                                                           >  8:234041/1‑103 (MQ=255)
                                                       t‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGt                                                                                                                                           <  7:234041/103‑1 (MQ=255)
                                                       t‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGt                                                                                             <  3:78097/149‑1 (MQ=255)
                                                         aCCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGt                                                                                             >  4:65553/1‑148 (MQ=255)
                                                          cccAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTc                                                                                            <  3:344971/148‑1 (MQ=255)
                                                            cAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTa                                                                                                                                          <  1:129613/100‑1 (MQ=255)
                                                            cAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTa                                                                                                                                          >  2:129613/1‑100 (MQ=255)
                                                               ggTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCa                                                                                      >  5:15651/1‑149 (MQ=255)
                                                                gTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGgtg                                                                                                                 <  4:209475/121‑1 (MQ=255)
                                                                gTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCAc                                                                                     >  6:188275/1‑149 (MQ=255)
                                                                 ttCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCAcc                                                                                    <  5:182790/149‑1 (MQ=255)
                                                                     aaCCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCctgc                                                                                <  3:105115/149‑1 (MQ=255)
                                                                     aaCCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCctgc                                                                                <  3:205585/149‑1 (MQ=255)
                                                                        cGCCAGGCTGGAACGACAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAg                                                                                                              >  5:213465/1‑116 (MQ=255)
                                                                        cGCCAGGCTGGAACGACAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAg                                                                                                              <  6:213465/116‑1 (MQ=255)
                                                                        cGCCAGGCTGGAACAGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTgg                                                                             <  4:217539/149‑1 (MQ=255)
                                                                           cAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGtt                                                                           >  8:54878/1‑148 (MQ=255)
                                                                             ggCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAg                                                                                                              <  8:330254/111‑1 (MQ=255)
                                                                             ggCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAg                                                                                                              >  7:330254/1‑111 (MQ=255)
                                                                                tGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTcagcag                                                                     >  4:296186/1‑149 (MQ=255)
                                                                                  gAACGGCAGCGTACCGTTCAGTGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCt                                                                                                     <  5:369927/115‑1 (MQ=255)
                                                                                  gAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCt                                                                                                     >  6:369927/1‑115 (MQ=255)
                                                                                  gAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGt                                                                                                 <  4:103043/119‑1 (MQ=255)
                                                                                  gAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGt                                                                                                 >  3:103043/1‑119 (MQ=255)
                                                                                  gAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAgg                                                                    <  6:15651/148‑1 (MQ=255)
                                                                                   aaCGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTc                                                                  >  2:293754/1‑149 (MQ=255)
                                                                                          gCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCtt                                                           <  4:152125/149‑1 (MQ=255)
                                                                                          gCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGACATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCtt                                                           <  5:147364/149‑1 (MQ=255)
                                                                                            gTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCTTTc                                                         <  7:54878/149‑1 (MQ=255)
                                                                                                     aGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCTTTCCACGCCAAt                                                >  3:242759/1‑149 (MQ=255)
                                                                                                      gggTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCTTTCCACGCCAATc                                               <  5:188275/149‑1 (MQ=255)
                                                                                                        gTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCg                                                                                                   <  7:138100/95‑1 (MQ=255)
                                                                                                        gTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCg                                                                                                   >  8:138100/1‑95 (MQ=255)
                                                                                                            gCTGCGGCAGGCGATCATACGGCGTGGATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGc                                                                                       >  1:187263/1‑104 (MQ=255)
                                                                                                            gCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGc                                                                                       <  2:187263/103‑1 (MQ=255)
                                                                                                             cTGCGACAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCTTTCCACGCCAATCTGGTCg                                         >  6:368178/1‑148 (MQ=255)
                                                                                                                cGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCTTTCCACGCCAATCTGGTCGGAt                                      <  3:55106/148‑1 (MQ=255)
                                                                                                                cGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCTTTCCACGCCAATCTGGTCGGAt                                      <  3:95178/148‑1 (MQ=255)
                                                                                                                        gATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCTTGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGATCAGCAGGTCGCGGCTTTCCACGCCAATCTCGTCGGATTCCAGGTcc                             >  1:136187/1‑149 (MQ=255)
                                                                                                                        gATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCTTTCCACGCCAATCTGGTCGGATTCCAGGTcc                             <  4:242759/149‑1 (MQ=255)
                                                                                                                            aTACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCTTTCCACGCCAATCTGGTCGGATTCCAGGTCCTGGa                         <  3:65553/149‑1 (MQ=255)
                                                                                                                             tACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGAACCAGCAgggt                                                                  >  4:160228/1‑105 (MQ=255)
                                                                                                                             tACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGAACCAGCAgggt                                                                  <  3:160228/107‑3 (MQ=255)
                                                                                                                               cGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCTTTCCACGCCAATCTGGTCGGATTCCAGGTCCTGGAAGt                      <  6:1200/149‑1 (MQ=255)
                                                                                                                                          tacGAGATGGCGACCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCTTTCCACGCCAATCTGGTCGGATTCCAGGTCCTGGaa                        >  8:233099/3‑137 (MQ=255)
                                                                                                                                          tacGAGATGGCGACCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCTTTCCACGCCAATCTGGTCGGATTCCAGGTCCTGGaa                        <  7:233099/135‑1 (MQ=255)
                                                                                                                                          tGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCTTTCCACGCCAATCTGGTCGGATTCCAGGTCCTGGAAGTAGAACGGGTc            >  2:210051/1‑149 (MQ=255)
                                                                                                                                            cGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCTTTCCACGCCAATCTGGTCGGATTCCAGGTCCTGGAAGTAGAACGGGTCg           <  3:296186/148‑1 (MQ=255)
                                                                                                                                            cGAGATGGCGACCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCTTTCCACGCCAATCTGGTCGGATTCCAGGTCCTGGAAGTAGAACGGGTCGc          >  8:116907/1‑149 (MQ=255)
                                                                                                                                                    tCGCCTTCTCGTAGGCATGCACATTCAACCGCGAGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCTTTCCACGCCAATCTGGTCGGATTCCAGGTCCTGGAAGTAGAACGGGTCGCTGATGTCg  <  2:136187/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATCAAAGACGGCACTGTCCTTCAGATCACGATCAAAGCGAACAGCTTCAGTCTCGT‑ACCCAAGGTTCAAACCGCCAGGCTGGAACGGCAGCGTACCGTTCAGGGTCAGCTGCGGCAGGCGATCATACGGCGT‑GATCTGCGAGATGGTCGCCATCTCGTAGGCATGCACATTCAACCGCGCGGTGTAGCTGTCGCCTCGGTAGGTCAACGCACCCTGCTGGTTCAGCAGGTCGCGGCTTTCCACGCCAATCTGGTCGGATTCCAGGTCCTGGAAGTAGAACGGGTCGCTGATGTCG  >  NC_002947/490870‑491164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑