Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 494,811 C→T 8.3% A191V (GCG→GTG)  djlA → DnaJ‑like protein DjlA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947494,8110CT 8.3% 134.0 / 2.5 48A191V (GCG→GTG) djlADnaJ‑like protein DjlA
Reads supporting (aligned to +/- strand):  ref base C (22/22);  new base T (2/2);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCAAGATGGGCAACAAGGAGCGTGAGTTGCTGCTGGACTGGGGGCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTAC  >  NC_002947/494663‑494955
                                                                                                                                                    |                                                                                                                                                
ggCAAGATGGGCAACAAGGAGCGTGAGTTGCTGCTGGACTGGGGGCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGc                                                                                                                                                  >  2:97695/1‑149 (MQ=255)
        gggCAACAAGGAGCGTGAGTTGCTGCTGGACTGGGGGCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCt                                                                                                                                          >  7:182689/1‑149 (MQ=255)
         ggCAACAAGGAGCGTGAGTTGCTGCTGGACTGGGGGCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCtt                                                                                                                                         <  1:97695/149‑1 (MQ=255)
            aacaaGGAGCGTGAGTTGCTGCTGGACTGGGGGCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCt                                                                                                                                      >  3:261748/1‑149 (MQ=255)
            aacaaGGAGCGTGAGTTGCTGCTGGACTGGGGGCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCt                                                                                                                                      >  6:361980/1‑149 (MQ=255)
              caaGGAGCGTGAGTTGCTGCTGGAATGGGGGCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTgg                                                                                                                                    >  3:253787/1‑149 (MQ=255)
               aaGGAGCGTGAGTTGCTGCTGGACTGGGGGCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGtt                                                                                                                                               <  4:126688/137‑1 (MQ=255)
               aaGGAGCGTGAGTTGCTGCTGGACTGGGGGCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGtt                                                                                                                                               >  3:126688/1‑137 (MQ=255)
                 ggAGCGTGAGTTGCTGCTGGACTGGGGGCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCgg                                                                                                                                 >  6:214211/1‑149 (MQ=255)
                   aGCGTGAGTTGCTGCTGGACTGGGGGCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTg                                                                                                                               <  1:355412/149‑1 (MQ=255)
                         aGTTGCTGCTGGACTGGGGGCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCa                                                                                                                         <  4:253787/149‑1 (MQ=255)
                             gctgctGGACTGGGGGCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAgac                                                                                                                      <  5:214211/148‑1 (MQ=255)
                                actgGACTGGGGGCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCg                                                                                                                                  <  6:165384/132‑1 (MQ=255)
                                actgGACTGGGGGCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCg                                                                                                                                  >  5:165384/2‑133 (MQ=255)
                                    gACTCGGGGCAGACCCTGGCCAGGAGCCGGCGCCAGGTGCCGGCCTTGGCCCTTGAGAACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAgacaccgacac                                                                                                              <  4:261748/149‑1 (MQ=255)
                                       tGGGGGCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAgacaccgacac                                                                                                              <  5:306422/146‑1 (MQ=255)
                                       tGGGGGCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAgacaccgacac                                                                                                              >  6:306422/1‑146 (MQ=255)
                                            gCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAgg                                                                                                      >  3:121696/1‑149 (MQ=255)
                                                    tGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGcc                                                                                               >  4:341000/1‑147 (MQ=255)
                                                     gggCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCACGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAgg                                                                                             >  1:83588/1‑149 (MQ=255)
                                                      ggCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGc                                                                                            <  5:19479/149‑1 (MQ=255)
                                                         aTGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGACACTGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTa                                                                                         <  8:182689/149‑1 (MQ=255)
                                                           gAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGc                                                                                            <  3:461/144‑1 (MQ=255)
                                                           gAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGc                                                                                            >  4:461/1‑144 (MQ=255)
                                                              ccGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTAtcggc                                                                                    <  5:255640/149‑3 (MQ=255)
                                                                   gcCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCtt                                                                               <  4:24971/149‑1 (MQ=255)
                                                                        gTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGc                                                                                                                                       >  4:5547/1‑88 (MQ=255)
                                                                        gTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGc                                                                                                                                       <  3:5547/88‑1 (MQ=255)
                                                                          gCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGTGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGcc                                                                        >  4:80484/1‑149 (MQ=255)
                                                                          gCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGTGTTGCGATTGCTGGCGGGGGACGCAGACACCGACACCGATAAGGTCAATCAGGCGTATCGTCGGCTTGTAAgac                                                                        >  4:75561/1‑147 (MQ=255)
                                                                                  tGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGTGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCAc                                                                <  3:80484/149‑1 (MQ=255)
                                                                                  tGTCCCTGGAGTACGAGCCGAGCAAGGCCACGGTAGCAGAAAGCGTGCCCATGACCTACGCGGCAGTGTTGCGCTTTCTGGCGGGGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCATCCGGCATCAc                                                                <  3:75561/149‑1 (MQ=255)
                                                                                    tCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCAccc                                                              >  4:341164/1‑149 (MQ=255)
                                                                                      ccTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCgg                                                            <  4:121696/149‑1 (MQ=255)
                                                                                        tGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGAc                                                          >  2:295517/1‑149 (MQ=255)
                                                                                               cGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTgg                                                   >  2:64887/1‑149 (MQ=255)
                                                                                                gAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGc                                                  <  5:361980/149‑1 (MQ=255)
                                                                                                             ccACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGcc                                     >  4:328809/1‑149 (MQ=255)
                                                                                                                   gAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAg                               <  1:64887/149‑1 (MQ=255)
                                                                                                                       aGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGgcg                            >  2:193127/1‑148 (MQ=255)
                                                                                                                         aaaGCGTGCCCAGGCCCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCGTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAg                         <  7:326493/149‑1 (MQ=255)
                                                                                                                                  ccATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGaa                <  1:295517/149‑1 (MQ=255)
                                                                                                                                  ccATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGaa                <  3:328809/149‑1 (MQ=255)
                                                                                                                                   cATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGt                   >  3:93301/1‑145 (MQ=255)
                                                                                                                                   cATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGt                   <  4:93301/145‑1 (MQ=255)
                                                                                                                                        ccTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGt                       >  2:345380/1‑136 (MQ=255)
                                                                                                                                        ccTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGt                       <  1:345380/136‑1 (MQ=255)
                                                                                                                                          tACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCACGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACcgc        >  5:20182/1‑149 (MQ=255)
                                                                                                                                                gCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTAc  >  5:38644/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GGCAAGATGGGCAACAAGGAGCGTGAGTTGCTGCTGGACTGGGGGCAGAAGCTGGGCATGAGCCGGCGCCAGGTGCAGGCCATGTCCCTGGAGTACGAGCCGCGCAAGGCCACGGGAGCAGAAAGCGTGCCCATGACCTACGCGGCAGCGTTGCGCTTGCTGGCGGTGGACGCAGACACCGACACCGATAAGGTCAAGCAGGCGTATCGTCGGCTTGTCAGCCGGCATCACCCGGACAAGCTGGCAGGTACCGGTGCCAGCGAGGCGCAGGTGCGTGAAGCAACCGCGCGTAC  >  NC_002947/494663‑494955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑