Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 524,365 C→T 11.5% intergenic (+232/‑584) tyrS → / → PP_16SC tyrosine‑‑tRNA ligase/16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947524,3650CT11.5% 135.1 / 6.6 52intergenic (+232/‑584)tyrS/PP_16SCtyrosine‑‑tRNA ligase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (24/22);  new base T (3/3);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

GGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCG  >  NC_002947/524221‑524503
                                                                                                                                                |                                                                                                                                          
gggagggggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCAt                                                                                                                                        <  8:42036/149‑1 (MQ=255)
  gagggggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGACTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGAGGCGCTCGGTCTCATAg                                                                                                                                      >  4:270113/1‑149 (MQ=255)
                  gagGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGt                                                                                                                      >  8:73725/1‑149 (MQ=255)
                     ggTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTgg                                                                                                                    <  1:128389/148‑1 (MQ=255)
                      gtgtTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGc                                                                                                                   >  1:132131/1‑148 (MQ=255)
                                    aTGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGcc                                                                                                    >  1:194033/1‑149 (MQ=255)
                                      gtgtCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTc                                                                                                  >  3:84506/1‑149 (MQ=255)
                                               acaACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTc                                                                                         <  7:109287/149‑1 (MQ=255)
                                                    ccTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTg                                                                                                                     <  2:238259/116‑1 (MQ=255)
                                                        gCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTcccc                                                                                 <  2:132131/148‑1 (MQ=255)
                                                        gCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTccc                                                                                  <  2:194033/147‑1 (MQ=255)
                                                                 gtgAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCa                                                                       >  8:323370/1‑149 (MQ=255)
                                                                  tgAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCa                                                                       <  4:84506/148‑1 (MQ=255)
                                                                  tgAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCAc                                                                      >  4:306233/1‑149 (MQ=255)
                                                                   gagaTCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACt                                                                     >  6:279568/1‑149 (MQ=255)
                                                                         gAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCtt                                                                >  3:323976/1‑148 (MQ=255)
                                                                                  ccgcGTCCGACTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAg                                                      <  3:270113/149‑1 (MQ=255)
                                                                                   cgcgTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAgg                                                     <  4:323976/149‑1 (MQ=255)
                                                                                      gTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCACCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTta                                                                              <  3:44678/121‑1 (MQ=255)
                                                                                      gTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCACCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTta                                                                              >  4:44678/1‑121 (MQ=255)
                                                                                       tCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTc                                                                                                  >  2:328803/1‑100 (MQ=255)
                                                                                       tCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTc                                                                                                  <  1:328803/100‑1 (MQ=255)
                                                                                           gCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTc                                                                                    >  6:268528/1‑110 (MQ=255)
                                                                                           gCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTc                                                                                    <  5:268528/110‑1 (MQ=255)
                                                                                               gagaCATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGAc                                                <  1:281077/142‑1 (MQ=255)
                                                                                               gagaCATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAAATGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGAc                                                >  2:281077/1‑142 (MQ=255)
                                                                                                 gaCATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCtt                                                                                       >  6:145223/1‑101 (MQ=255)
                                                                                                    aTTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCgctt                                    >  3:85579/1‑146 (MQ=255)
                                                                                                    aTTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAAt                                    <  3:306233/149‑1 (MQ=255)
                                                                                                     ttCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATc                                   >  5:229394/1‑149 (MQ=255)
                                                                                                       catcGCCTATGCGACCGAGCGCCGCCCTCGCGGCGCTCGGTTTCATAGGCGGTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATccc                                 <  8:333837/146‑1 (MQ=11)
                                                                                                        aCCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCctct                                         <  2:206281/140‑1 (MQ=255)
                                                                                                        aCCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCctct                                         >  1:206281/1‑140 (MQ=255)
                                                                                                            ccTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTtat                            <  6:229394/149‑1 (MQ=255)
                                                                                                             cTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATccc                                 >  7:64384/1‑143 (MQ=255)
                                                                                                             cTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATccc                                 <  8:64384/143‑1 (MQ=255)
                                                                                                             cTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTtat                            <  8:120865/148‑1 (MQ=255)
                                                                                                             cTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTtat                            >  7:120865/1‑148 (MQ=255)
                                                                                                                   aaCCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATgcgc                     <  5:279568/149‑1 (MQ=255)
                                                                                                                     ccGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGaaa                                                            >  2:293193/1‑108 (MQ=255)
                                                                                                                     ccGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGaaa                                                            <  1:293193/108‑1 (MQ=255)
                                                                                                                      cGAGCGCCGCCCGCGCGGCGCTCGGTTTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGcccc                  >  2:32505/1‑149 (MQ=255)
                                                                                                                      cGAGCGCCGCCCGCGCGGCGCTCGGTTTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGccc                   >  7:285467/1‑148 (MQ=255)
                                                                                                                       gAGCGCCGCCCGCGCGGCGCTCGGTTTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGcccc                  >  4:19723/1‑148 (MQ=255)
                                                                                                                               cccgcgcggcgCTCGGTTTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGc         <  3:19723/144‑1 (MQ=255)
                                                                                                                                  gggcggCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGa                                                 >  6:296649/1‑106 (MQ=38)
                                                                                                                                  gggcggCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGa                                                 <  5:296649/106‑1 (MQ=38)
                                                                                                                                  gggcggCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGa       >  2:182431/1‑148 (MQ=40)
                                                                                                                                      ggcgCTCGGTTTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCGTTATAATGCGCCCCACTTCCAGCGACATCg  <  1:32505/149‑1 (MQ=255)
                                                                                                                                       gcgcTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCg  >  4:154451/1‑148 (MQ=255)
                                                                                                                                           tCGGTCTCATAGGCGCTGAAATTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGc         >  7:254615/1‑137 (MQ=255)
                                                                                                                                           tCGGTCTCATAGGCGCTGAAATTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGc         <  8:254615/137‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                          
GGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCACTTCCAGCGACATCG  >  NC_002947/524221‑524503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑