Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	537,461	T→A	6.1%	noncoding (57/66 nt)	PP_mr07 →	P26 ncRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	537,461	0	T	A	6.1%	199.1 / 2.6	66	noncoding (57/66 nt)	PP_mr07	P26 ncRNA
Reads supporting (aligned to +/- strand):  ref base T (30/32);  new base A (2/2);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

GTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACC  >  NC_002947/537316‑537606                                                                                                                                                   |                                                                                                                                                  gTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGCCTGATGGCTGGTGGCTTATGc                                                                                                                                                 >  3:95484/1‑148 (MQ=255)    gTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCAc                                                                                                                                              <  5:360633/148‑1 (MQ=255)        cggAAGGGGTTTCGAACTAAGCCGCTGAAGACGCTAAGATCACGCTGGAAGAAGCAGGCGCTAAAGGCGAGCTTAAGTAATCTCTACCTTGCGATGCCAGCCT‑GAGCGTTGAGCTAAAGGCTGATGGCCGGTGGCATAAGCCACCAGc                                                                                                                                          <  8:140854/146‑1 (MQ=255)        cTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGcc                                                                                                                                         <  7:228586/149‑1 (MQ=255)        cTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCATAAGCCAc                                                                                                                                              <  4:309763/144‑1 (MQ=255)        cTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCATAAGCCAc                                                                                                                                              >  3:309763/1‑144 (MQ=255)        cTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCCGGTGGCATAAGCCACCAGc                                                                                                                                          >  7:140854/1‑148 (MQ=255)                 tttCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGt                                                                                                                                >  5:144793/1‑149 (MQ=255)                   tCGAAAGAAGCCGCTTAAGACGCTATGAAGAAGCTGTAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGtt                                                                                                                               <  6:224790/148‑1 (MQ=255)                     gAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGTCACCGGCCTTTTTCCGTTAtt                                                                                                                            <  5:306226/149‑1 (MQ=255)                     gAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTAtt                                                                                                                            >  6:185878/1‑149 (MQ=255)                     gAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTAtt                                                                                                                            >  2:208544/1‑149 (MQ=255)                     gAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTAtt                                                                                                                            <  2:22709/149‑1 (MQ=255)                             ccGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCg                                                                                                                    <  6:349799/149‑1 (MQ=255)                               gCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGt                                                                                                                  >  1:85052/1‑149 (MQ=255)                                   aaGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAgg                                                                                                              >  1:132798/1‑149 (MQ=255)                                    aGACGCTAAGAAGAAGCTGCAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAgg                                                                                                              <  1:353842/148‑1 (MQ=255)                                        gCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGt                                                                                                         >  4:330166/1‑149 (MQ=255)                                          tAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTcc                                                                                                       >  6:68844/1‑149 (MQ=255)                                           aagaagaagCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTccc                                                                                                      >  5:324072/1‑149 (MQ=255)                                            agaagaagCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCg                                                                                                     <  3:330166/149‑1 (MQ=255)                                                   gCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAAc                                                                                                >  8:244798/1‑147 (MQ=255)                                                   gCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGa                                                                                              <  6:324072/149‑1 (MQ=255)                                                        aagaagCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGa                                                                                                    <  7:308070/138‑1 (MQ=255)                                                        aagaagCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGa                                                                                                    >  8:308070/1‑138 (MQ=255)                                                          gaagCAGGCGCTAAAGGCGAGCTGAAGTAATCTCGACCTTGCGATCCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCaa                                                                                       <  7:114446/149‑1 (MQ=255)                                                           aagCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGc                                                                                         >  2:130338/1‑146 (MQ=255)                                                               aGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGGGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGacac                                                                                  <  2:132798/149‑1 (MQ=255)                                                                  cgcTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCAGCCAGaaaatcg                                                                               >  6:149929/1‑143 (MQ=255)                                                                     tAAAGTCGATCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGATGATGGATGGTGGCTTATGCCACAGGCCTTATTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGATGCAAGACACCCAtgt                                                                            >  5:210829/1‑149 (MQ=255)                                                                     tAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCTGGAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCAtg                                                                             >  1:69356/1‑149 (MQ=255)                                                                     tAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCAtgt                                                                            >  1:293084/1‑149 (MQ=255)                                                                          tCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATgtgggtg                                                                       <  2:85052/149‑1 (MQ=255)                                                                            gAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCATGACACCCATGTGGGTGgc                                                                     >  8:142807/1‑149 (MQ=255)                                                                            gAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCACatgggtgtc                                                                     <  4:196085/149‑3 (MQ=255)                                                                              gCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGgcg                                                                    >  2:133982/1‑148 (MQ=255)                                                                                     tAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCaa                                                                                       <  6:104920/122‑1 (MQ=255)                                                                                     tAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCaa                                                                                       >  5:104920/1‑122 (MQ=255)                                                                                      aaTCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCCGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAg                                                           >  6:167815/1‑149 (MQ=255)                                                                                          tcGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGt                                                       <  5:3225/149‑1 (MQ=255)                                                                                            gACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGttt                                                     <  1:133982/149‑1 (MQ=255)                                                                                               cTTGCTATGCCATCCG‑GAGCGTTGAGCGAATGGCTGTTGTCTGGTGGCTAATGCCACCGGCCTTTTTCCGTTATTGGGGGCCGGTCAGGCCGGTGCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAATCCAAGGGGTTTg                                                    <  8:326734/147‑1 (MQ=255)                                                                                                  gCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGa                                               >  2:174887/1‑149 (MQ=255)                                                                                                         cAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTg                                        <  5:185878/149‑1 (MQ=255)                                                                                                          aGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAATGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTgg                                       >  3:173642/1‑149 (MQ=255)                                                                                                          aGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTgg                                       <  7:244798/149‑1 (MQ=255)                                                                                                            ccT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGgc                                                                     >  1:200358/1‑117 (MQ=255)                                                                                                            ccT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGgc                                                                     <  2:200358/117‑1 (MQ=255)                                                                                                             cT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGgct                                     >  5:43812/1‑148 (MQ=255)                                                                                                                 aGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTg                                                    >  6:188605/1‑130 (MQ=255)                                                                                                                 aGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTg                                                    <  5:188605/130‑1 (MQ=255)                                                                                                                  gCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTcc                                >  3:214463/1‑149 (MQ=255)                                                                                                                   cGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAATGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTccc                               <  4:173642/149‑1 (MQ=255)                                                                                                                    gTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCccg                              >  8:195902/1‑149 (MQ=255)                                                                                                                     ttGAGCGAAAGGCCGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCccgc                             <  5:167815/149‑1 (MQ=255)                                                                                                                        aGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg                          <  7:195902/149‑1 (MQ=255)                                                                                                                        aGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCgg                          <  2:293084/149‑1 (MQ=255)                                                                                                                          cGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGga                        <  1:208544/149‑1 (MQ=255)                                                                                                                             aaGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGaa                     <  4:255903/149‑1 (MQ=255)                                                                                                                             aaGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGaa                     >  3:255903/1‑149 (MQ=255)                                                                                                                                 cTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGTCa                 <  1:174887/149‑1 (MQ=255)                                                                                                                                        cTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGc          <  7:142807/149‑1 (MQ=255)                                                                                                                                           gtggCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGTGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAg        <  4:95484/148‑1 (MQ=255)                                                                                                                                                ttATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGaa                     >  8:145697/1‑130 (MQ=255)                                                                                                                                                ttATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGaa                     <  7:145697/130‑1 (MQ=255)                                                                                                                                                  aTGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGAcc  >  3:232275/1‑147 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GTTGTAGCTGAAGGCGTTTCGAAAGAAGCCGCTGAAGACGCTAAGAAGAAGCTGGAAGAAGCAGGCGCTAAAGTCGAGCTGAAGTAATCTCGACCTTGCGATGCCAGCCT‑GAGCGTTGAGCGAAAGGCTGATGGCTGGTGGCTTATGCCACCGGCCTTTTTCCGTTATTGGTGGCCGGTCAGGCCGGTCCCGATAACGAGCTGCAAGACACCCATGTGGGTGGCGCAAACCAAGGGGTTTGCACGATTTTCTGGCTGCTCCCGCCGGGAGAAGCCAAACAAGCAGGTGACC  >  NC_002947/537316‑537606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑