Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	593,434	A→C	7.4%	E391A (GAG→GCG) ‡	PP_0503 →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	593,434	0	A	C	7.4%	193.5 / 7.5	68	E391A (GAG→GCG) ‡	PP_0503	MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (28/35);  new base C (3/2);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 6.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.09e-01

TTCGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCG  >  NC_002947/593291‑593573                                                                                                                                                |                                                                                                                                            ttCGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGgccg                                                                                                                                        <  8:49311/149‑1 (MQ=255)     cTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTAGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCt                                                                                                                                    >  3:253853/1‑149 (MQ=255)          cAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAAt                                                                                                                               >  4:105967/1‑149 (MQ=255)            gCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCTCGGCCGCCCt                                                                                                                                    <  4:144336/142‑1 (MQ=255)            gCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCTCGGCCGCCCt                                                                                                                                    >  3:144336/1‑142 (MQ=255)             cGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGtt                                                                                                                            <  8:222905/149‑1 (MQ=255)               gTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACAGTGGGGGTGGGTTGCGGTGTGTGGGCTTGGCATGGTCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGtt                                                                                                                            >  5:173627/1‑147 (MQ=255)               gTTTGGCGGCGGTGTTGGCGAGCTGGGTGTTAGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATCGGCATCAGTGCCGCCGCACTGTGCTACTCGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGtt                                                                                                                            >  8:39644/1‑147 (MQ=255)                tttGGGGGCGGTGTTGGCGAGCTGGGTGGTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAg                                                                                                                         <  3:72613/149‑1 (MQ=255)                       gCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGaa                                                                                                                  >  4:326418/1‑149 (MQ=255)                       gCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGaa                                                                                                                  <  3:202468/149‑1 (MQ=255)                       gCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTCGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGaa                                                                                                                  <  4:253853/149‑1 (MQ=255)                           tgtTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTg                                                                                                              >  4:311372/1‑149 (MQ=255)                             ttGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTa                                                                                                            <  1:10645/149‑1 (MQ=255)                                  gAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGcc                                                                                                         >  6:241936/1‑147 (MQ=255)                                        ggTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAg                                                                                                                         >  5:6586/1‑125 (MQ=255)                                        ggTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAg                                                                                                                         <  6:6586/125‑1 (MQ=255)                                              tGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATg                                                                                            >  5:116707/1‑148 (MQ=255)                                                 acacTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGtata                                                                                        >  3:35384/1‑149 (MQ=255)                                                   acTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATAcc                                                                                      >  7:144250/1‑149 (MQ=255)                                                   acTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATAcc                                                                                      >  1:45161/1‑149 (MQ=255)                                                       gggCTGGGTTGCGGTGTGTGGGCTTGGGATGGGCATGTGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCTTGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGAGCATGTATACCCAc                                                                                   <  7:39644/148‑1 (MQ=255)                                                       gggCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACg                                                                                  >  8:161253/1‑149 (MQ=255)                                                         gCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGAACGACCATGt                                                                                           >  6:204529/1‑138 (MQ=255)                                                         gCTGGGTTGCGGTGTGGGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGt                                                                                           <  5:204529/138‑1 (MQ=255)                                                          cTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgt                                                                               <  6:173627/149‑1 (MQ=255)                                                                   ggTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcga                                                                      >  8:322364/1‑149 (MQ=255)                                                                      gtgtgGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgacgt                                                                   <  8:144250/149‑1 (MQ=255)                                                                      gtgtgGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGATACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgacgt                                                                   <  6:144228/149‑1 (MQ=255)                                                                       tgtgGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgacgtc                                                                  <  6:116707/149‑1 (MQ=255)                                                                           ggCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATAc                                                                                       >  3:98049/1‑124 (MQ=255)                                                                           ggCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATAc                                                                                       <  4:98049/124‑1 (MQ=255)                                                                            gCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCt                                                             >  1:81041/1‑149 (MQ=255)                                                                            gCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCt                                                             >  3:250514/1‑149 (MQ=255)                                                                             cTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCt                                                                                                                                    <  1:151408/77‑1 (MQ=255)                                                                             cTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCt                                                                                                                                    >  2:151408/1‑77 (MQ=255)                                                                                ggCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTgg                                                          >  2:56389/1‑148 (MQ=255)                                                                                ggCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTgg                                                          <  7:322364/148‑1 (MQ=255)                                                                                 gCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTc                                                        >  7:147078/1‑149 (MQ=255)                                                                                  cATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAg                                                                                                                         >  2:127559/1‑83 (MQ=255)                                                                                  cATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAg                                                                                                                         <  1:127559/83‑1 (MQ=255)                                                                                  cATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtc                                                                              <  6:352063/126‑1 (MQ=255)                                                                                  cATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtc                                                                              >  5:352063/1‑126 (MQ=255)                                                                                   ctGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTg                                                           <  4:139424/143‑1 (MQ=255)                                                                                   aTGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGCCCTTggagat                                                      >  3:139424/1‑145 (MQ=255)                                                                                      ggCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGc                                                    <  5:241936/148‑1 (MQ=255)                                                                                      ggCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCt                                                   <  3:326418/149‑1 (MQ=255)                                                                                          tCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAg                                               <  8:256121/149‑1 (MQ=255)                                                                                          acAGTGCGGCGGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgt                                                                               <  1:193263/116‑1 (MQ=255)                                                                                          acAGTGCGGCGGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgt                                                                               >  2:193263/2‑117 (MQ=255)                                                                                             gTGCCGCCGCACTGTGCTACTGGCTTTGGCTGCAACGCGCGTGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCa                                            <  5:169563/149‑1 (MQ=255)                                                                                              tGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCaa                                           <  7:161253/149‑1 (MQ=255)                                                                                                      cACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTc                                   <  2:45161/149‑1 (MQ=255)                                                                                                           tgCTACTTGGTATGGCTGCACCGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTg                                                     <  2:5892/126‑1 (MQ=255)                                                                                                           tgCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGCCCGTGGTCGTg                                                     >  1:5892/1‑126 (MQ=255)                                                                                                           tgCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCggg                                <  2:81041/147‑1 (MQ=255)                                                                                                           tgCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCggg                                <  4:250514/147‑1 (MQ=255)                                                                                                                cTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCggg                                <  6:26090/142‑1 (MQ=255)                                                                                                                cTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCggg                                >  5:26090/1‑142 (MQ=255)                                                                                                                 tGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgacgtcga                                                                >  3:193900/1‑109 (MQ=255)                                                                                                                       tGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAACACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTgg                   <  4:35384/148‑1 (MQ=255)                                                                                                                         gcggCAACGCGCGCGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGcc                 <  8:147078/145‑1 (MQ=255)                                                                                                                                 gcgcgAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcg        <  1:56389/149‑1 (MQ=255)                                                                                                                                   gcgAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgat      <  1:141064/149‑1 (MQ=255)                                                                                                                                     caGGGCGGCCTCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgatgt    >  4:175522/2‑149 (MQ=255)                                                                                                                                      aGGGCGGCCTCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgacgtcga                                                                >  1:31057/1‑88 (MQ=255)                                                                                                                                      aGGGCGGCCTCGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGgtcgacgtcgacgtcga                                                                <  2:31057/88‑1 (MQ=255)                                                                                                                                       gggCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGtgtcgatgtcg  <  3:105967/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                            TTCGCTGGGCAGCGGTTTGGGGGCGGTGTTGGCGAGCTGGGTGTTTGCACACTGGGGCTGGGTTGCGGTGTGTGGGCTTGGCATGGGCATCAGTGCCGCCGCACTGTGCTACTGGCTATGGCTGCAACGCGCGAGGGCGGCCGAGGCCGCCCTGCAATGTTCAGGTCAGAACTTGTAGCCAAGACCGACCATGTATACCCACGGGTCGACGTCGACGTCGACCTTGGTCTTGCTGTAGCCAAGGGCGGTCGGGCCGTTGACGCTGGCCTTGGTGTCGATGTCG  >  NC_002947/593291‑593573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑