Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 600,244 T→G 8.2% V24G (GTC→GGC)  nrdR → DNA‑binding transcriptional repressor NrdR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947600,2440TG 8.2% 145.2 / 4.1 49V24G (GTC→GGC) nrdRDNA‑binding transcriptional repressor NrdR
Reads supporting (aligned to +/- strand):  ref base T (24/21);  new base G (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

GGGCATATTTCCCATGGATGTTAACCCCTTTGGCTTGTCTTGCCAGTGCAATGCGCTACCATGGCCCTCCCTTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAGCAGGACGGCACGCGCCAGCCCTTCGACGAAGAAAAGCTGCGCGC  >  NC_002947/600098‑600391
                                                                                                                                                   |                                                                                                                                                   
gggCATATTTCCCATGGATGTTAACCCCTTTGGCTTGTCTTGCCAGTGCAATGCGCTACCATGGCCCTCCCTTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCtcgtcg                                                                                                                                                   >  1:153338/1‑149 (MQ=255)
     tatTTCCCATGGATGTTAACCCCTTTGGCTTGTCTTGCCAGTGCAATGCGCTACCATGGCCCTCCCTTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAg                                                                                                                                              <  6:176248/149‑1 (MQ=255)
         tCCCATTGATGTTTAAGCCTTTGGATTGTCTTGGCAGTGCAATGCGCTACCATGGCCCTCCCTTTTTTTGTTGCCTGCCACCTTGCACTGTCCCTTTTGGGTTGCCAACGACACCATGGTCATCGACTCTCG‑CCTCGTCGCCTATGGc                                                                                                                                           <  6:222424/148‑1 (MQ=255)
         tCCCATGGATGTCAACCCCTTTGGCTTGTCTTGCCAGTGCAATGCGCTACCCTGGCCCTCCCTTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGc                                                                                                                                           <  6:341077/148‑1 (MQ=255)
           ccATGGATGTTAACCCCTTTGGCTTGTCTTGCCAGTGCAATGCGCTACCATGGCCCTCCCTTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAg                                                                                                                                        >  2:127705/1‑149 (MQ=255)
              tGGATGTTAACCCCTTTGGCTTGTCTTGCCAGTGCAATGCGCTACCATGGCCCTCCCTTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCGCCCTCGGCGACGAggcgagagt                                                                                                                                      <  7:239668/149‑8 (MQ=255)
                    ttAACCCCTTTGGCTTGTCTTGCCAGTGCAATGCGCTACCATGGCCCTCCCTTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTgcg                                                                                                                                <  8:232373/148‑1 (MQ=255)
                           cTTTGGCTTGTCTTGCCAGTGCAATGCGCTACCATGGCCCTCCCTTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCgccgccgcc                                                                                                                        >  6:284175/1‑149 (MQ=255)
                               ggCTTGTCTTGCCAGTGCAATGCGCTACCATGGCCCTCCCTTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTg                                                                                                                     <  5:284175/148‑1 (MQ=255)
                                 cTTGTCTTGCCAGTGCAATGCGCTACCATGGCCCTCCCTTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAAt                                                                                                                  >  5:352489/1‑149 (MQ=255)
                                    gTCTTGCCAGTGCAATGCGCTACCATGGCCCTCCCTTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGccgccgccg                                                                                                                       >  5:76475/1‑141 (MQ=255)
                                    gTCTTGCCAGTGCAATGCGCTACCATGGCCCTCCCTTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGccgccgccg                                                                                                                       <  6:76475/141‑1 (MQ=255)
                                    gTCTTGCCAGTGCAATGCGCTACCATGGCCCTCCCTTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCg                                                                                                               >  2:58530/1‑149 (MQ=255)
                                              tGCAATGCGCTACCATGGCCCTCCCTTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTgcgg                                                                                                     <  7:19205/149‑1 (MQ=255)
                                               gCAATGCGCTACCATGGCCCTCCCTTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTgcggc                                                                                                    >  3:102443/1‑149 (MQ=255)
                                                          ccATGGCCCTCCCTTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTc                                                                                           <  6:352489/147‑1 (MQ=255)
                                                          ccATGGCCCGCCGATTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTcac                                                                                         <  4:197704/149‑1 (MQ=255)
                                                           cATGGCCCTCCCTTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTcac                                                                                         >  5:281821/1‑148 (MQ=255)
                                                                     cctTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGaa                                                                               <  2:153338/148‑1 (MQ=255)
                                                                       tttttttGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTgc                                                                                                                                 >  2:177070/1‑96 (MQ=255)
                                                                       tttttttGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTgc                                                                                                                                 <  1:177070/96‑1 (MQ=255)
                                                                       tttttttGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAAcc                                                                            >  6:137511/1‑149 (MQ=255)
                                                                         tttttGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAAccg                                                                           <  6:281821/148‑1 (MQ=255)
                                                                            ttgttgCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAAccgcc                                                                         >  5:140983/1‑147 (MQ=255)
                                                                                   ctgccACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAAcc                                                                            <  4:28239/137‑1 (MQ=255)
                                                                                   ctgccACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAAcc                                                                            >  3:28239/1‑137 (MQ=255)
                                                                                   ctgccACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGtg                                                                <  4:35260/149‑1 (MQ=255)
                                                                                       caccaTGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGtgct                                                              <  1:127705/147‑1 (MQ=255)
                                                                                                   tCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATc                                                 >  1:136126/1‑148 (MQ=255)
                                                                                                     ccTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAg                                              >  2:355696/1‑149 (MQ=255)
                                                                                                      cTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAg                                              >  3:220425/1‑148 (MQ=255)
                                                                                                      cTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAGc                                             <  6:140983/149‑1 (MQ=255)
                                                                                                             ggTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAGCAGGACgg                                      >  6:231821/1‑149 (MQ=255)
                                                                                                               tGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGtgctgc                                                            <  4:251121/125‑1 (MQ=255)
                                                                                                                   aaCGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAGCAGGACGGCAcgcg                                >  3:213587/1‑149 (MQ=255)
                                                                                                                         acCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAGCAGGACGGCACGCGCCAGcc                          >  3:304257/1‑149 (MQ=255)
                                                                                                                          ccAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAGCAGGACGGCACGCGCCAGccc                         >  2:233360/1‑149 (MQ=255)
                                                                                                                           cAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTcacc                                                                                        >  3:327964/1‑85 (MQ=255)
                                                                                                                           cAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTcacc                                                                                        <  4:327964/85‑1 (MQ=255)
                                                                                                                           cAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAGCAGGACGGCACGCGCCAGCCCt                        >  5:96122/1‑149 (MQ=255)
                                                                                                                                 cATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAGCAGGACGGCACGCGCCAGCCCTTcgac                   >  3:13307/1‑148 (MQ=255)
                                                                                                                                   tCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAGCAGGACGGCACGCGCCAGCCCTTcgacg                  >  4:223933/1‑147 (MQ=255)
                                                                                                                                   tCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAGCAGGACGGCACGCGCCAGCCCTTCGACgaa                <  5:137511/149‑1 (MQ=255)
                                                                                                                                        tctcG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCg                                                                                                               <  2:290898/49‑1 (MQ=255)
                                                                                                                                          tcgcccTCGGCGACGA‑GGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAGCAGGACGGCACGCGCCAGCCCTTCGACGAAGAAAAGc         >  5:339674/5‑149 (MQ=255)
                                                                                                                                          tcG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAGCAGGACGGCACGCGCCAGCCCTTCGACGAAGaaaa           <  4:304257/147‑1 (MQ=255)
                                                                                                                                          tcG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAGCAGGACGGCACGCGCCAGCCCTTCGACGAAGAAAAGc         >  2:221289/1‑149 (MQ=255)
                                                                                                                                           cgcccTCGGCGACGA‑GGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAGCAGGACGGCACGCGCCAGCCCTTcga                    >  6:222559/4‑137 (MQ=255)
                                                                                                                                           cgcccTCGGCGACGA‑GGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAGCAGGACGGCACGCGCCAGCCCTTcga                    <  5:222559/134‑1 (MQ=255)
                                                                                                                                            g‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAGCAGGACGGCACGCGCCAGCCCTTCGACGAAGAAAAGCTg       >  4:12994/1‑149 (MQ=255)
                                                                                                                                                  gtcgCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAGCAGGACGGCACGCGCCAGCCCTTCGACGAAGAAAAGCTgcgcgc  >  2:322423/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGGCATATTTCCCATGGATGTTAACCCCTTTGGCTTGTCTTGCCAGTGCAATGCGCTACCATGGCCCTCCCTTTTTTTGTTGCCTGCCACCATGCACTGTCCCTTTTGCGGTGCCAACGACACCAAGGTCATCGACTCTCG‑CCTCGTCGCCGAGGGCGAGCAGGTGCGCCGCCGCCGTGAATGCGTGGCCTGCGGCGAGCGCTTCACCACCTTCGAAACCGCCGAGCTGGTGCTGCCCCGGCTGATCAAGCAGGACGGCACGCGCCAGCCCTTCGACGAAGAAAAGCTGCGCGC  >  NC_002947/600098‑600391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑