Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 630,319 G→A 7.7% S458S (AGC→AGT)  PP_0544 ← ethanolamine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947630,3190GA 7.7% 150.3 / 2.7 52S458S (AGC→AGT) PP_0544ethanolamine transporter
Reads supporting (aligned to +/- strand):  ref base G (21/27);  new base A (2/2);  total (23/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

TGTGTACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCACCCGGG  >  NC_002947/630179‑630460
                                                                                                                                            |                                                                                                                                             
tgtgtACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGaa                                                                                                                                       <  5:209145/149‑1 (MQ=255)
  tgtACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGaaa                                                                                                                                      <  1:226243/148‑1 (MQ=255)
  tgtACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGaaa                                                                                                                                      <  4:35443/148‑1 (MQ=255)
    tACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGc                                                                                                                                    >  6:120330/1‑148 (MQ=255)
      cGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCaa                                                                                                                                  <  7:81999/148‑1 (MQ=255)
         aaCTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTa                                                                                                                              <  6:266333/149‑1 (MQ=255)
          aCTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAg                                                                                                                             <  6:306766/149‑1 (MQ=255)
            ttGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGc                                                                                                                           <  2:149634/149‑1 (MQ=255)
               ccATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCaa                                                                                                                         <  8:61662/148‑1 (MQ=255)
                       aaTCTCCAGAGCCGCGGGTTGTCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGTATCGCGGCGCATTCCTCTTCCGGGGTGCCCGCAACCAAGTGGTGGCGACTTTAGAAAGCAAAGTAGGCAATTAAGAcag                                                                                                                >  5:360676/1‑147 (MQ=255)
                       aaTCTCCAGAGCCGCGGGTTGTCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGACTGTAGAAAGCAAAGTAGGCAATTAATACCg                                                                                                                >  5:365566/1‑149 (MQ=255)
                             cAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCa                                                                                                              <  5:120330/145‑1 (MQ=255)
                                agCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGc                                                                                                                           <  8:81631/129‑1 (MQ=255)
                                agCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGc                                                                                                                           >  7:81631/1‑129 (MQ=255)
                                  ccGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACt                                                                                                     <  4:241570/149‑1 (MQ=255)
                                  ccGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACt                                                                                                     >  6:160205/1‑149 (MQ=255)
                                  ccGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACt                                                                                                     <  8:15341/149‑1 (MQ=255)
                                           gtcccGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGACTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCa                                                                                            <  6:365566/147‑1 (MQ=255)
                                           gCCCCGCGGCGGGTGGCGTCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGcc                                                                                             <  6:252054/148‑1 (MQ=255)
                                           gCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGGAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGcc                                                                                             <  6:256488/148‑1 (MQ=255)
                                            tcccGCGGCGGGTGGCGGCGAGCAGGGAAGTGCCTCTTCGTCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGACGGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCa                                                                                            <  6:360676/147‑1 (MQ=255)
                                            ccccGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCaa                                                                                           >  3:55018/1‑149 (MQ=255)
                                              ccGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGACTTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGAGCACTGCAGCGCCAAt                                                                                          >  3:37986/1‑148 (MQ=255)
                                                        tGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCcgcg                                                                               <  5:133906/149‑1 (MQ=255)
                                                              cgATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCaa                                                                         <  4:55018/149‑1 (MQ=255)
                                                                        aGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATc                                                                                                        <  4:31142/108‑1 (MQ=255)
                                                                        aGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATc                                                                                                        >  3:31142/1‑108 (MQ=255)
                                                                         gggCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAAt                                                                                                                    >  5:247999/1‑95 (MQ=255)
                                                                         gggCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAAt                                                                                                                    <  6:247999/95‑1 (MQ=255)
                                                                               ctTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGAc                                                         >  5:353479/1‑148 (MQ=255)
                                                                                       ttCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAg                                                                     <  3:311319/128‑1 (MQ=255)
                                                                                       ttCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAg                                                                     >  4:311319/1‑128 (MQ=255)
                                                                                            gATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACg                                           >  6:260178/1‑149 (MQ=255)
                                                                                            gATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACg                                           >  6:164544/1‑149 (MQ=255)
                                                                                              ccgcgGCGAATTCCTCTTCCGGGGTGACCTCAACCTAGTGCTCGCGGATTTAGACAGCAAAGTAGGCAATTACTACCGCATAGATCACTGCACCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGcc                                         <  4:225243/148‑1 (MQ=255)
                                                                                                gcggcgAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAg                                       >  6:53469/1‑149 (MQ=255)
                                                                                                 cggcgAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGc                                      >  2:216867/1‑149 (MQ=255)
                                                                                                        ttCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAAc                                                                        <  8:320423/108‑1 (MQ=255)
                                                                                                        ttCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAAc                                                                        >  7:320423/1‑108 (MQ=255)
                                                                                                           ctctTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGcc                             <  6:353479/148‑1 (MQ=255)
                                                                                                                 cGGTGTGCCCGGATACTAGTGTTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATATATCACTGCAGCGCCAATGACTTCCCGAGGGTCAAGAATGAATGCGGCTACCACGGCGATGCACGCCAGCTCCAGCGCCAGGCCg                       <  4:37986/148‑1 (MQ=255)
                                                                                                                   gtgtGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGAt                    <  5:160205/149‑1 (MQ=255)
                                                                                                                        cccGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGAtgtg                 >  1:108835/1‑147 (MQ=255)
                                                                                                                        cccGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGAtgtg                 >  4:11152/1‑147 (MQ=255)
                                                                                                                         ccGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCAc                                            <  6:141548/119‑1 (MQ=255)
                                                                                                                         ccGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCAc                                            >  5:141548/1‑119 (MQ=255)
                                                                                                                         ccGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGAtgtg                 >  7:75765/1‑146 (MQ=255)
                                                                                                                          cGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGAtgtg                 >  6:6445/1‑145 (MQ=255)
                                                                                                                               ccAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCa        >  3:299643/1‑149 (MQ=255)
                                                                                                                                cAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCTCTGCAGCGCCAATGACTACCCGCGGGTCAACCAGTAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCAc       <  8:75765/149‑1 (MQ=255)
                                                                                                                                cAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCAc       <  4:299643/149‑1 (MQ=255)
                                                                                                                                 aaGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCAcc      >  6:110050/1‑149 (MQ=255)
                                                                                                                                          cggcTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCACCCggg  >  5:196186/1‑144 (MQ=255)
                                                                                                                                          cggcTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCACCCggg  >  5:201181/1‑144 (MQ=255)
                                                                                                                                          cggcTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCACCCggg  <  6:201181/144‑1 (MQ=255)
                                                                                                                                          cggcTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCACCCggg  <  6:196186/144‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                             
TGTGTACGAAACTTGCCATACAGAATCTCCAGAGCCGCGGGTTGCCCCGCGGCGGGTGGCGGCGATCAGTGCAGGGCCTCTTCGGCCTTCTGGATCGCGGCGAATTCCTCTTCCGGTGTGCCCGCAACCAAGTGGTGGCGGCTGTAGAAAGCAAAGTAGGCAATTAATACCGCATAGATCACTGCAGCGCCAATGACTACCCGCGGGTCAACCAGGAATCCGGCGACCACGGCGATGCACGCCAGCACCAGCGCCAGGCCGGATGTGAAGATGCCACCCGGG  >  NC_002947/630179‑630460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑