Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 36,671 A→G 7.4% W328R (TGG→CGG)  PP_0033 ← undecaprenyl‑glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294736,6710AG 7.4% 149.7 / 2.7 54W328R (TGG→CGG) PP_0033undecaprenyl‑glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (26/24);  new base G (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

CCCGCTGATTCTGAGGGCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGTAGAAACAAG  >  NC_002947/36527‑36816
                                                                                                                                                |                                                                                                                                                  
cccGCTGATTCTGAGGGCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCt                                                                                                                                                >  6:250050/1‑149 (MQ=255)
 ccGCTGATTCTGAGGGCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCt                                                                                                                                                <  3:215138/148‑1 (MQ=255)
        ttCTGAGGGCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAgc‑gc                                                                                                                                       >  2:45396/1‑149 (MQ=255)
                           ttACCCCGGCGTTCATGATGCCCGCACCTATCATTGCCAACACCAATGCGCTCGCAGCGGCAGCCCAGTTGGTCTTCGGGCCAATTTCGCTTACCCGTGCATACAGCAGCAATGCCCACGCTGGTAGC‑GCGGGTAGCAGGTAGGTCca                                                                                                                     <  7:54083/147‑1 (MQ=255)
                           gTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTc                                                                                                                       <  4:232729/146‑1 (MQ=255)
                           gTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTc                                                                                                                       <  5:250050/146‑1 (MQ=255)
                             aCCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCaggtag                                                                                                                          <  5:254176/141‑1 (MQ=255)
                             aCCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCaggtag                                                                                                                          >  6:254176/1‑141 (MQ=255)
                               cccGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCcaca                                                                                                                   <  5:283257/146‑1 (MQ=255)
                               cccGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCcaca                                                                                                                   >  6:283257/1‑146 (MQ=255)
                               cccGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGaa                                                                                                                >  4:181794/1‑149 (MQ=255)
                                     gTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGc                                                                                                          <  2:216482/149‑1 (MQ=255)
                                      tAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGcc                                                                                                         <  1:26027/149‑1 (MQ=255)
                                      tAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGCGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGcc                                                                                                         <  4:351769/149‑1 (MQ=255)
                                              gCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAAtg                                                                                                 >  8:25870/1‑149 (MQ=255)
                                                        atgatTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGAc                                                                                       >  8:339265/1‑149 (MQ=255)
                                                         tgatTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGTAGAACAcc                                                                                      >  4:69103/1‑149 (MQ=255)
                                                               gCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAg                                                                                                                  >  3:298217/1‑115 (MQ=255)
                                                               gCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAg                                                                                                                  <  4:298217/115‑1 (MQ=255)
                                                               gCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTga                                                                                              <  6:202104/135‑1 (MQ=255)
                                                               gCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTga                                                                                              >  5:202104/1‑135 (MQ=255)
                                                               gCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTc                                                                                >  5:355278/1‑149 (MQ=255)
                                                                 aaCACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGgt                                                                                   >  4:141725/1‑144 (MQ=255)
                                                                 aaCACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGgt                                                                                   <  3:141725/144‑1 (MQ=255)
                                                                      cAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAAt                                                                                                  >  7:333144/1‑124 (MQ=255)
                                                                      cAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAAt                                                                                                  <  8:333144/124‑1 (MQ=255)
                                                                      cAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAgg                                                                         <  6:355278/149‑1 (MQ=255)
                                                                      cAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAAAGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAAAGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAgg                                                                         >  5:140552/1‑149 (MQ=255)
                                                                                gCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCa                                                               <  1:45396/149‑1 (MQ=255)
                                                                                              ggtggtCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTg                                                  >  8:308834/1‑148 (MQ=255)
                                                                                               gtggtCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGaa                                                <  7:339265/149‑1 (MQ=255)
                                                                                                  gtCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGt                                                          >  5:211540/1‑136 (MQ=255)
                                                                                                  gtCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGt                                                          <  6:211540/136‑1 (MQ=255)
                                                                                                     ttCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTg                                          >  3:199211/1‑149 (MQ=255)
                                                                                                       cGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTTTAAATGTGAAGAAGACCGGTGGCGCCATGGCGCTCATCCAGAGGTAGGCTTTGAAGCGCTg                                          <  6:167391/147‑1 (MQ=255)
                                                                                                              aCTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGtt                                                                                                            <  5:367087/74‑1 (MQ=255)
                                                                                                              aCTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGtt                                                                                                            >  6:367087/1‑74 (MQ=255)
                                                                                                                         ccGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGa                      >  3:284374/1‑149 (MQ=255)
                                                                                                                          cGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCaa                                                                           >  1:256051/1‑95 (MQ=255)
                                                                                                                          cGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCaa                                                                           <  2:256051/95‑1 (MQ=255)
                                                                                                                          cGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGAt                     <  3:181794/149‑1 (MQ=255)
                                                                                                                           gTGCAGACAGCAGCAATGCCCACGCTGGCAGCTGCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGCGAAGAAGACCGGTGTCGCCACGGCCCACAGCCCGAGGTAGGCTTAGAAGCGCTGCATCGATCCACGCTTGCGGAt                     >  1:223660/1‑149 (MQ=255)
                                                                                                                           gTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGAt                     >  7:154857/1‑148 (MQ=255)
                                                                                                                             gCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCaa                  >  5:252334/1‑149 (MQ=255)
                                                                                                                                   agcagcAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCAt                                       <  3:147354/122‑1 (MQ=255)
                                                                                                                                   agcagcAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCAt                                       >  4:147354/1‑122 (MQ=255)
                                                                                                                                     cagcaATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGTa          <  4:284374/149‑1 (MQ=255)
                                                                                                                                      agcaATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGaa                                                <  7:180170/110‑1 (MQ=255)
                                                                                                                                      agcaATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGaa                                                >  8:180170/1‑110 (MQ=255)
                                                                                                                                        tgctacccGCGCTGCCAGC‑GTGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGa                      >  2:225229/6‑134 (MQ=255)
                                                                                                                                        tgctacccGCGCTGCCAGC‑GTGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGa                      <  1:225229/129‑1 (MQ=255)
                                                                                                                                             cccGCGCTGCCAGC‑GTGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGt           >  3:134978/1‑140 (MQ=255)
                                                                                                                                             cccGCGCTGCCAGC‑GTGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGt           <  4:134978/140‑1 (MQ=255)
                                                                                                                                             cccACGCTGGCAGC‑GCGGGTAGCACGTAGCTCCACAGAATGTAGCCTGAAAATCTGAAGAAGACCGATGTCGCCAAGGCCCACAGCCAGAGGTAGCCTTTGAAGCGCTTCATCGATCCACGATTCCGGATCAAAAGTCGTAGAAACAAg  >  8:185964/1‑149 (MQ=255)
                                                                                                                                                aCGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTgg            <  2:122396/136‑1 (MQ=255)
                                                                                                                                                aCGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTga            >  1:122396/1‑135 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CCCGCTGATTCTGAGGGCGCTCCTCTAGTACCCCGGCGTAGATGATGCCCGCACCGATGATTGGCAACACCAATGCGCTCGCAGCGGCAGCCCAGGTGGTCTTCGGGCCAACTTCGCTGACCCGTGCAGACAGCAGCAATGCCCACGCTGGCAGC‑GCGGGTAGCAGGTAGGTCCACAGAATGTTGCCTGCAAATGTGAAGAAGACCGGTGTCGCCAAGGCCCACAGCCAGAGGTAGGCTTTGAAGCGCTGCATCGATCCACGCTTGCGGATCAACAGTGGTAGAAACAAG  >  NC_002947/36527‑36816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑