Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	672,002	C→T	10.5%	L353L (CTG→TTG)	PP_0573 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	672,002	0	C	T	10.5%	101.0 / 2.4	38	L353L (CTG→TTG)	PP_0573	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (18/16);  new base T (2/2);  total (20/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.73e-01

AACTGCTGCTGTCGGCGTCGCGCTACCGCAGCGAGCCCGGCACGCGCATACGTTTGGACGATGGCCGCGATATCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGCAAATACGACACCTATGTGCGGGCGCTGTCATTCGAAGGTGACTGGCGCAAGGTGGAGCAGGGGCGTTTGCG  >  NC_002947/671856‑672147                                                                                                                                                   |                                                                                                                                                  aaCTGCTGCTGTCGGCGTCGCGCTACCGCAGCGAGCCCGGCACGCGCATACGTTTGGACGATGGCCGCGATATCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGtggctg                                                                                                                                                 >  2:73737/1‑149 (MQ=255)      ctgctgTCGGCGTCGCGCTACCGCAGCGAGCCCGGCACGCGCATACGTTTGGACGATGGCCGCGATATCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGt                                                                                                                                            >  2:186365/1‑149 (MQ=255)           gTCGGCGTCGCGCTACCGCAGCGAGCCCGGCACGCGCATACGTTTGGACGATGGCCGCGATATCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAgg                                                                                                                                       >  4:277221/1‑149 (MQ=255)           gTCGGCGTCGCGCTACCGCAGCGAGCCCGGCACGCGCATACGTTTGGACGATGGCCGCGATATCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAgg                                                                                                                                       >  2:137168/1‑149 (MQ=255)            tCGGCGTCGCGCTACCGCAGCGAGCCCGGCACGCGCATACGTTTGGACGATGGCCGCGATATCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAgg                                                                                                                                       <  8:48361/148‑1 (MQ=255)            tCGGCGTCGCGCTACCGCAGCGAGCCCGGCACGCGCATACGTTTGGACGATGGCCGCGATATCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAgg                                                                                                                                       <  2:278093/148‑1 (MQ=255)                       cTACCGCAGCGAGCCCGGCACGCGCATACGTTTGGACGATGGCCGCGATATCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACt                                                                                                                           <  3:277221/149‑1 (MQ=255)                          ccGCAGCGAGCCCGGCACGCGCATACGTTTGGACGATGGCCGCGATATCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCgg                                                                                                                        >  5:285889/1‑149 (MQ=255)                              aGCGAGCCCGGCACGCGCATACGTTTGGACGATGGCCGCGATATCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAg                                                                                                                    >  7:32328/1‑149 (MQ=255)                                    cccGGCACGCGCATACGTTTGGACGATGGCCGCGATATCACCCGAGCGCACGACAGTGAGCCCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTGAGGCGGAATGAGTGGCTGGCGGTGCACGGCGACCTGTACTCGGTCAGTGAGc                                                                                                               >  8:269782/1‑148 (MQ=255)                                     ccGGCACGCGCATACGTTTGGACGATGGCCGCGATATCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCAc                                                                                                                                  <  8:93024/128‑1 (MQ=255)                                     ccGGCACGCGCATACGTTTGGACGATGGCCGCGATATCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCAc                                                                                                                                  >  7:93024/1‑128 (MQ=255)                                       ggCACGCGCATACGTTTGGACGATGGCCGCGATATCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCatga                                                                                                           >  7:337350/1‑149 (MQ=255)                                                     tttGGACGATGGCCGCGATATCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATgg                                                                                             <  6:285889/149‑1 (MQ=255)                                                          aCGATGGCCGCGATATCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGag                                                                                        >  7:168885/1‑149 (MQ=255)                                                            gttGGCGGCGATATCACCCGGGATCACGAGAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGagca                                                                                      <  8:337350/147‑1 (MQ=255)                                                             aTGGCCGCGATATCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGcc                                                                                           <  8:285855/143‑1 (MQ=255)                                                             aTGGCCGCGATATCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGcc                                                                                           >  7:285855/1‑143 (MQ=255)                                                                       tatCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGtctc                                                                           <  1:73737/149‑1 (MQ=255)                                                                         tCACCCGCGAGCTCGACTGTGAGCGCTATTCGAGCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGTTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGc                                                                         <  8:168885/149‑1 (MQ=255)                                                                             ccGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGTTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGagca                                                                                      <  1:113383/132‑1 (MQ=255)                                                                             ccGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGTTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGagca                                                                                      <  1:109298/132‑1 (MQ=255)                                                                             ccGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGTTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGagca                                                                                      >  2:113383/1‑132 (MQ=255)                                                                             ccGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGTTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGagca                                                                                      >  2:109298/1‑132 (MQ=255)                                                                             ccGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGCaaa                                                                      >  1:155434/1‑148 (MQ=255)                                                                                   gCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGCAAATACGaca                                                               >  1:331236/1‑149 (MQ=255)                                                                                    cACGAGAGTGCGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGATTGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGGCAGTGCGCATGATGAAGTCCGGATGGCCGAGCAGCCAACGTCTCGCAAATACGaca                                                               <  7:269782/148‑1 (MQ=255)                                                                                      cGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGCAAATACGACACCt                                                            <  2:331236/149‑1 (MQ=255)                                                                                      cGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGCAAATACGACACCt                                                            <  8:32328/149‑1 (MQ=255)                                                                                           gTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGCAAATACGACACCTAtgtg                                                       <  1:137168/149‑1 (MQ=255)                                                                                            tGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGAAAATACGACACCTATGTGc                                                      >  6:289154/1‑149 (MQ=255)                                                                                                        gATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGCAAATACGACACCTATGTGCgg                                                    >  7:280788/1‑139 (MQ=255)                                                                                                        gATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGCAAATACGACACCTATGTGCgg                                                    <  8:280788/139‑1 (MQ=255)                                                                                                        gATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGCAAATACGACACCTATGTGCGGGCGCTGTCAt                                          >  6:61260/1‑149 (MQ=255)                                                                                                             gCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGCAAATACGACACCTATGTGCGGGCGCTGTCATTCGaa                                     <  8:33606/149‑1 (MQ=255)                                                                                                                tGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGCAAATACGACACCTATGTGCGGGCGCTGTCATTCGAAGGt                                  >  7:295308/1‑149 (MQ=255)                                                                                                                  cGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGCAAATACGACACCTATGTGCGGGCGCTGTCATTCGAAGGt                                  <  5:61260/147‑1 (MQ=255)                                                                                                                     cAGCCCCTGGTTGACAGGCCGAATGAGTGGCTGGCCGTGCAGGGCCACCAGTACTCCGTCAGTGCGCATGATCAAGACCGGATGGCCGACCAGCCAACGTCTCGCAAAGACGACACCTATGTGCTGGCGCTGTCATTCTAAGGTGACTg                             >  6:119511/1‑149 (MQ=255)                                                                                                                                               gTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGCAAATACGACACCTATGTGCGGGCGCTGTCATTCGAAGGTGACTGGCGCAAGGTGGAGCAGGGGCGTTTGc   >  3:54119/1‑149 (MQ=255)                                                                                                                                                tggctggcGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGCAAATACGACACCTATGTGCGGGCGCTGTCATTCGAAGGTGACTGGCGCAAGGTGGAGCAGGGGCGTTTGCg  <  1:186365/149‑1 (MQ=255)                                                                                                                                                 ggctggcGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGCAAATACGACACCTATGTGCGGGCGCTGTCATTCGAAGGTGACTGGCGCAAGGTGGAGCAGGGGCGTTTGc   <  5:289300/147‑1 (MQ=255)                                                                                                                                                 ggctggcGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGCAAATACGACACCTATGTGCGGGCGCTGTCATTCGAAGGTGACTGGCGCAAGGTGGAGCAGGGGCGTTTGc   >  6:289300/1‑147 (MQ=255)                                                                                                                                                   |                                                                                                                                                  AACTGCTGCTGTCGGCGTCGCGCTACCGCAGCGAGCCCGGCACGCGCATACGTTTGGACGATGGCCGCGATATCACCCGCGAGCACGACAGTGAGCGCTATGCGATCGGCCTGCGTCAGCCGGTGGTTGTCAGGCCGAATGAGTGGCTGGCGGTGCAGGGCCACCTGTACTCGGTCAGTGAGCATGATGAAGACCGGATGGCCGAGCAGCCAACGTCTCGCAAATACGACACCTATGTGCGGGCGCTGTCATTCGAAGGTGACTGGCGCAAGGTGGAGCAGGGGCGTTTGCG  >  NC_002947/671856‑672147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑