Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 706,843 A→C 8.5% intergenic (+19/+227) PP_0599 → / ← rpsT paraquat‑inducible protein B/30S ribosomal protein S20

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947706,8430AC 8.5% 136.6 / 5.2 47intergenic (+19/+227)PP_0599/rpsTparaquat‑inducible protein B/30S ribosomal protein S20
Reads supporting (aligned to +/- strand):  ref base A (24/19);  new base C (2/2);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01

CAACGCCAGATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAGTCCCG  >  NC_002947/706706‑706990
                                                                                                                                         |                                                                                                                                                   
cAACGCCAGATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCt                                                                                                                                          >  4:317797/1‑149 (MQ=255)
 aaCGCCAGATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTg                                                                                                                                         <  2:325696/149‑1 (MQ=255)
     ccAGATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGCAGGGCCGCGCTTGGCGGCCCTACttt                                                                                                                                     <  4:350343/149‑1 (MQ=255)
       aGATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCttttt                                                                                                                                   >  1:202962/1‑149 (MQ=255)
        gATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCGCTTGGCGGCCCTACttttac                                                                                                                                  <  7:118608/149‑3 (MQ=255)
          tGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTtgt                                                                                                                                <  2:202962/149‑1 (MQ=255)
           ggCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTtgtg                                                                                                                               >  1:171685/1‑149 (MQ=255)
            gCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTgg                                                                                                                              <  2:294729/149‑1 (MQ=255)
            gCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTgg                                                                                                                              <  5:197390/149‑1 (MQ=255)
                  aGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCttt                                                                                                                                     >  8:339166/1‑136 (MQ=255)
                  aGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCttt                                                                                                                                     <  7:339166/136‑1 (MQ=255)
                  aGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCgg                                                                                                                        <  8:283609/149‑1 (MQ=255)
                    aTGGGTACCCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAACTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGcc                                                                                                                      >  1:293328/1‑149 (MQ=255)
                        gTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGc                                                                                                                  >  5:244874/1‑149 (MQ=255)
                             ccGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTcgc                                                                                                             >  7:146593/1‑149 (MQ=255)
                                   cgGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAgg                                                                                                       <  6:244874/149‑1 (MQ=255)
                                                      ccTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAACTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCg                                                                                    <  2:293328/149‑1 (MQ=255)
                                                                                 ggCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCg                                                         >  7:82194/1‑149 (MQ=255)
                                                                                    tGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATTTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCgggg                                                      <  8:146593/149‑1 (MQ=255)
                                                                                      cAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCt                                                    >  6:104687/1‑149 (MQ=255)
                                                                                          gggCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGccc                                                >  6:341647/1‑149 (MQ=255)
                                                                                          gggCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCATGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCCGGGCTGgcc                                                >  3:304359/1‑149 (MQ=255)
                                                                                           ggCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGcccc                                               >  1:305339/1‑149 (MQ=255)
                                                                                            gCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCg                                              <  5:104687/149‑1 (MQ=255)
                                                                                            gCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCg                                              <  2:171685/149‑1 (MQ=255)
                                                                                                cGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCt                                                    >  4:145671/1‑139 (MQ=255)
                                                                                                cGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCt                                                    <  3:145671/139‑1 (MQ=255)
                                                                                                cGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCATGCGTCGCGAAACGGGCGCGTAGCCGCCCCGCGGGCGACGGGCACAAAGTGTGATATCCGGGGCTGCCCCGCacg                                          >  4:4527/1‑147 (MQ=37)
                                                                                                   aGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAg                                           <  4:312381/145‑1 (MQ=255)
                                                                                                   aGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAg                                           >  3:312381/1‑145 (MQ=255)
                                                                                                     aTTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCACTCGGTCGCAGCGCAGAAAGTGAGATTGCCGGGGCAGCCCCGCAGCCCGAt                                     >  8:84577/1‑149 (MQ=255)
                                                                                                      ttCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATc                                    <  5:341647/149‑1 (MQ=255)
                                                                                                         aGATCGCCAAGTGAAGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATcgcg                                 >  5:101402/1‑149 (MQ=255)
                                                                                                          gATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTga                                                                 <  7:335108/116‑1 (MQ=255)
                                                                                                               ccAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGacac                             >  1:252729/1‑147 (MQ=255)
                                                                                                                cAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGAcaca                            >  5:331069/1‑147 (MQ=255)
                                                                                                                cAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGAcaca                            <  6:331069/147‑1 (MQ=255)
                                                                                                                  aGTTATGTAAACGTCGGGCCGCCACGCGCGGCCGTGCTTTTTTGTGGGCGCGGCCTTGGGTCGCTAACGGGGCGCTTAGCGGCCCCTCGGTCTCAGCGCACAACGTGACATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACACGGc                        <  2:305339/149‑1 (MQ=255)
                                                                                                                       caaaaaaGCAGGGCCGCGCTTGGCGGCCCTACTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTTAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTc                   >  4:254528/4‑149 (MQ=25)
                                                                                                                        aaaaaaGCAGGGCCGCGCTTGGCGGCCCTACTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTcc                  >  5:289401/3‑149 (MQ=25)
                                                                                                                           aaaGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCca                >  4:73122/1‑148 (MQ=255)
                                                                                                                             aGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCca                >  2:301885/1‑146 (MQ=255)
                                                                                                                                 gggCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATc          <  8:82194/148‑1 (MQ=255)
                                                                                                                                  ggCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTGCCACAGATc          >  5:310499/1‑147 (MQ=255)
                                                                                                                                   gccgccACGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGGCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAg       >  5:83598/1‑149 (MQ=255)
                                                                                                                                     cgccAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAGTc     <  3:73122/149‑1 (MQ=255)
                                                                                                                                        cAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAGTCCCg  >  3:174212/1‑149 (MQ=255)
                                                                                                                                         |                                                                                                                                                   
CAACGCCAGATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAGTCCCG  >  NC_002947/706706‑706990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑