Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 706,846 C→G 8.5% intergenic (+22/+224) PP_0599 → / ← rpsT paraquat‑inducible protein B/30S ribosomal protein S20

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947706,8460CG 8.5% 146.8 / 4.7 47intergenic (+22/+224)PP_0599/rpsTparaquat‑inducible protein B/30S ribosomal protein S20
Reads supporting (aligned to +/- strand):  ref base C (24/19);  new base G (2/2);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

CAACGCCAGATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAGTCCCGCAT  >  NC_002947/706706‑706993
                                                                                                                                            |                                                                                                                                                   
cAACGCCAGATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCt                                                                                                                                             >  4:317797/1‑149 (MQ=255)
 aaCGCCAGATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTg                                                                                                                                            <  2:325696/149‑1 (MQ=255)
     ccAGATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGCAGGGCCGCGCTTGGCGGCCCTACttt                                                                                                                                        <  4:350343/149‑1 (MQ=255)
       aGATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCttttt                                                                                                                                      >  1:202962/1‑149 (MQ=255)
        gATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCGCTTGGCGGCCCTACttttac                                                                                                                                     <  7:118608/149‑3 (MQ=255)
          tGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTtgt                                                                                                                                   <  2:202962/149‑1 (MQ=255)
           ggCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTtgtg                                                                                                                                  >  1:171685/1‑149 (MQ=255)
            gCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTgg                                                                                                                                 <  2:294729/149‑1 (MQ=255)
            gCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTgg                                                                                                                                 <  5:197390/149‑1 (MQ=255)
                  aGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCttt                                                                                                                                        >  8:339166/1‑136 (MQ=255)
                  aGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCttt                                                                                                                                        <  7:339166/136‑1 (MQ=255)
                  aGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCgg                                                                                                                           <  8:283609/149‑1 (MQ=255)
                    aTGGGTACCCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAACTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGcc                                                                                                                         >  1:293328/1‑149 (MQ=255)
                        gTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGc                                                                                                                     >  5:244874/1‑149 (MQ=255)
                             ccGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTcgc                                                                                                                >  7:146593/1‑149 (MQ=255)
                                   cgGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAgg                                                                                                          <  6:244874/149‑1 (MQ=255)
                                                      ccTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAACTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCg                                                                                       <  2:293328/149‑1 (MQ=255)
                                                                                 ggCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCg                                                            >  7:82194/1‑149 (MQ=255)
                                                                                    tGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATTTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCgggg                                                         <  8:146593/149‑1 (MQ=255)
                                                                                      cAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCt                                                       >  6:104687/1‑149 (MQ=255)
                                                                                          gggCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGccc                                                   >  6:341647/1‑149 (MQ=255)
                                                                                          gggCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCATGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCCGGGCTGgcc                                                   >  3:304359/1‑149 (MQ=255)
                                                                                           ggCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGcccc                                                  >  1:305339/1‑149 (MQ=255)
                                                                                            gCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCg                                                 <  5:104687/149‑1 (MQ=255)
                                                                                            gCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCg                                                 <  2:171685/149‑1 (MQ=255)
                                                                                                cGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCt                                                       >  4:145671/1‑139 (MQ=255)
                                                                                                cGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCt                                                       <  3:145671/139‑1 (MQ=255)
                                                                                                cGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCATGCGTCGCGAAACGGGCGCGTAGCCGCCCCGCGGGCGACGGGCACAAAGTGTGATATCCGGGGCTGCCCCGCacg                                             >  4:4527/1‑147 (MQ=37)
                                                                                                   aGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAg                                              <  4:312381/145‑1 (MQ=255)
                                                                                                   aGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAg                                              >  3:312381/1‑145 (MQ=255)
                                                                                                     aTTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCACTCGGTCGCAGCGCAGAAAGTGAGATTGCCGGGGCAGCCCCGCAGCCCGAt                                        >  8:84577/1‑149 (MQ=255)
                                                                                                      ttCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATc                                       <  5:341647/149‑1 (MQ=255)
                                                                                                         aGATCGCCAAGTGAAGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATcgcg                                    >  5:101402/1‑149 (MQ=255)
                                                                                                          gATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTga                                                                    <  7:335108/116‑1 (MQ=255)
                                                                                                               ccAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGacac                                >  1:252729/1‑147 (MQ=255)
                                                                                                                cAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGAcaca                               <  6:331069/147‑1 (MQ=255)
                                                                                                                cAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGAcaca                               >  5:331069/1‑147 (MQ=255)
                                                                                                                  aGTTATGTAAACGTCGGGCCGCCACGCGCGGCCGTGCTTTTTTGTGGGCGCGGCCTTGGGTCGCTAACGGGGCGCTTAGCGGCCCCTCGGTCTCAGCGCACAACGTGACATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACACGGc                           <  2:305339/149‑1 (MQ=255)
                                                                                                                       caaaaaaGCAGGGCCGCGCTTGGCGGCCCTACTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTTAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTc                      >  4:254528/4‑149 (MQ=25)
                                                                                                                        aaaaaaGCAGGGCCGCGCTTGGCGGCCCTACTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTcc                     >  5:289401/3‑149 (MQ=25)
                                                                                                                           aaaGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCca                   >  4:73122/1‑148 (MQ=255)
                                                                                                                             aGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCca                   >  2:301885/1‑146 (MQ=255)
                                                                                                                                 gggCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATc             <  8:82194/148‑1 (MQ=255)
                                                                                                                                  ggCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTGCCACAGATc             >  5:310499/1‑147 (MQ=255)
                                                                                                                                   gccgccACGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGGCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAg          >  5:83598/1‑149 (MQ=255)
                                                                                                                                     cgccAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAGTc        <  3:73122/149‑1 (MQ=255)
                                                                                                                                        cAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAGTCCCg     >  3:174212/1‑149 (MQ=255)
                                                                                                                                           gcgcGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCGGCCACAGATAGAGTCCCGCAt  >  1:143274/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
CAACGCCAGATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAGTCCCGCAT  >  NC_002947/706706‑706993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑