Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 706,855 G→A 11.7% intergenic (+31/+215) PP_0599 → / ← rpsT paraquat‑inducible protein B/30S ribosomal protein S20

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947706,8550GA11.7% 133.8 / 6.9 51intergenic (+31/+215)PP_0599/rpsTparaquat‑inducible protein B/30S ribosomal protein S20
Reads supporting (aligned to +/- strand):  ref base G (26/19);  new base A (3/3);  total (29/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

AACGCCAGATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAGTCCCGCATGGAACTGGG  >  NC_002947/706707‑707002
                                                                                                                                                    |                                                                                                                                                    
aaCGCCAGATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTg                                                                                                                                                      <  2:325696/149‑1 (MQ=255)
    ccAGATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGCAGGGCCGCGCTTGGCGGCCCTACttt                                                                                                                                                  <  4:350343/149‑1 (MQ=255)
      aGATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCttttt                                                                                                                                                >  1:202962/1‑149 (MQ=255)
       gATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCGCTTGGCGGCCCTACttttac                                                                                                                                               <  7:118608/149‑3 (MQ=255)
         tGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTtgt                                                                                                                                             <  2:202962/149‑1 (MQ=255)
          ggCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTtgtg                                                                                                                                            >  1:171685/1‑149 (MQ=255)
           gCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTgg                                                                                                                                           <  5:197390/149‑1 (MQ=255)
           gCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTgg                                                                                                                                           <  2:294729/149‑1 (MQ=255)
                 aGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCttt                                                                                                                                                  <  7:339166/136‑1 (MQ=255)
                 aGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCttt                                                                                                                                                  >  8:339166/1‑136 (MQ=255)
                 aGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCgg                                                                                                                                     <  8:283609/149‑1 (MQ=255)
                   aTGGGTACCCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAACTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑cc                                                                                                                                  >  1:293328/1‑149 (MQ=255)
                       gTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGc                                                                                                                              >  5:244874/1‑149 (MQ=255)
                            ccGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTcgc                                                                                                                         >  7:146593/1‑149 (MQ=255)
                                  cgGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAgg                                                                                                                   <  6:244874/149‑1 (MQ=255)
                                                     ccTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAACTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCg                                                                                                <  2:293328/149‑1 (MQ=255)
                                                                                ggCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCg                                                                     >  7:82194/1‑149 (MQ=255)
                                                                                   tGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATTTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCgggg                                                                  <  8:146593/149‑1 (MQ=255)
                                                                                     cAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCt                                                                >  6:104687/1‑149 (MQ=255)
                                                                                         gggCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGccc                                                            >  6:341647/1‑149 (MQ=255)
                                                                                         gggCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCATGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCCGGGCTGgcc                                                            >  3:304359/1‑149 (MQ=255)
                                                                                          ggCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGcccc                                                           >  1:305339/1‑149 (MQ=255)
                                                                                           gCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCg                                                          <  2:171685/149‑1 (MQ=255)
                                                                                           gCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCg                                                          <  5:104687/149‑1 (MQ=255)
                                                                                               cGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCt                                                                >  4:145671/1‑139 (MQ=255)
                                                                                               cGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCt                                                                <  3:145671/139‑1 (MQ=255)
                                                                                               cGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCATGCGTCGCGAAACGGGCGCGTAGCCGCCCCGCGGGCGACGGGCACAAAGTGTGATATCCGGGGCTGCCCCGCacg                                                      >  4:4527/1‑147 (MQ=37)
                                                                                                  aGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAg                                                       <  4:312381/145‑1 (MQ=255)
                                                                                                  aGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAg                                                       >  3:312381/1‑145 (MQ=255)
                                                                                                    aTTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCACTCGGTCGCAGCGCAGAAAGTGAGATTGCCGGGGCAGCCCCGCAGCCCGAt                                                 >  8:84577/1‑149 (MQ=255)
                                                                                                     ttCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATc                                                <  5:341647/149‑1 (MQ=255)
                                                                                                        aGATCGCCAAGTGAAGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATcgcg                                             >  5:101402/1‑149 (MQ=255)
                                                                                                         gATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTga                                                                             <  7:335108/116‑1 (MQ=255)
                                                                                                              ccAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGacac                                         >  1:252729/1‑147 (MQ=255)
                                                                                                               cAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGAcaca                                        >  5:331069/1‑147 (MQ=255)
                                                                                                               cAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGAcaca                                        <  6:331069/147‑1 (MQ=255)
                                                                                                                 aGTTATGTAAACGTCGGGCCGCCACGCGCGGCCGTGCTTTTTTGTGGGCGCGG‑CCTTGGGTCGCTAACGGGGCGCTTAGCGGCCCCTCGGTCTCAGCGCACAACGTGACATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACACGGc                                    <  2:305339/149‑1 (MQ=255)
                                                                                                                      caaaaaaGCAGGGCCGCGCTTGGCGGCCCTACTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTTAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTc                               >  4:254528/4‑149 (MQ=25)
                                                                                                                       aaaaaaGCAGGGCCGCGCTTGGCGGCCCTACTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTcc                              >  5:289401/3‑149 (MQ=25)
                                                                                                                          aaaGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCca                            >  4:73122/1‑148 (MQ=255)
                                                                                                                            aGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCca                            >  2:301885/1‑146 (MQ=255)
                                                                                                                                gggCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATc                      <  8:82194/148‑1 (MQ=255)
                                                                                                                                 ggCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTGCCACAGATc                      >  5:310499/1‑147 (MQ=255)
                                                                                                                                  gccgccACGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGGCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAg                   >  5:83598/1‑149 (MQ=255)
                                                                                                                                    cgccAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAGTc                 <  3:73122/149‑1 (MQ=255)
                                                                                                                                       gcttggcgGCCCTACTTTTTTGTGGGAGCGGTCCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAGTccc               >  1:218415/6‑149 (MQ=255)
                                                                                                                                       cAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAGTCCCg              >  3:174212/1‑149 (MQ=255)
                                                                                                                                          gcgcGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCGGCCACAGATAGAGTCCCGCAt           >  1:143274/1‑149 (MQ=255)
                                                                                                                                           ggcgGCCCTACTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAGTCCCGCAt           <  6:187268/147‑1 (MQ=255)
                                                                                                                                            gcgGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAGTCCCGCATgg         <  4:174212/149‑1 (MQ=255)
                                                                                                                                                 ccTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAGTCCCGCATGGAACTg    >  5:263104/1‑149 (MQ=255)
                                                                                                                                                   tGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAGTCCCGCATGGAACTggg  >  5:94233/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AACGCCAGATGGCGAGCAGATGGGTAACCCGGCGCGGCCGCAGCAGACCTTTGCCTTGTTCGAAAAGGCCGAGGATGAATGGCTGCAGTGGGCACCGAAGATTCAGATCGCCAAGTGATGTAAAAGTAGGGCCGCCAAGCGCGGCCCTGCTTTTTTGTGGGAGCGG‑CCTTGCGTCGCGAAAGGGGCGCGTAGCGGCCCCTCGGTCTCAGCGCAGAAAGTGAGATTGCCGGGGCTGCCCCGCAGCCCTATCGCGACACAAGGCCGCTCCCACAGATCGAGTCCCGCATGGAACTGGG  >  NC_002947/706707‑707002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑