| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 714,998 | T→A | 7.0% | intergenic (+64/+13) | ispH → / ← PP_0607 | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase/type IV pili biogenesis protein FimT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 714,998 | 0 | T | A | 7.0% | 169.7 / 2.8 | 57 | intergenic (+64/+13) | ispH/PP_0607 | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase/type IV pili biogenesis protein FimT |
| Reads supporting (aligned to +/- strand): ref base T (26/27); new base A (2/2); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
ACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTGCGCCCAACCAGCTGTTGCCC > NC_002947/714851‑715143 | aCTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTg < 3:5087/149‑1 (MQ=255) gcgcTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGAGACACAAGACCGCTCCTGCAAt > 8:303279/1‑149 (MQ=255) gACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTagcag > 1:46048/1‑149 (MQ=255) gACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCAGCAAT‑GACCGCTGCTagcg > 6:230921/1‑148 (MQ=255) cGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGCGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑g > 1:255626/1‑149 (MQ=255) gcgcGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGt > 3:348908/1‑148 (MQ=255) cgcgAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCa < 5:181553/149‑1 (MQ=255) cgAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGCTGCTAGC‑GC‑GGTCAtt < 5:269161/149‑1 (MQ=255) agagAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCt < 8:230880/149‑1 (MQ=255) agaACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCa < 3:325700/149‑1 (MQ=255) gaACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCAGCAAT‑GACCGCTGCTAGC‑GC‑GGTCATTGcagga < 5:230921/149‑5 (MQ=255) gaACATCACCTTATCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAg < 7:63015/149‑1 (MQ=255) tCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGtcct > 7:306998/1‑149 (MQ=255) tCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGtcct < 2:46048/149‑1 (MQ=255) cACCTTTTCAATGCCCAAAGAGCTGCTGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCAGCAAT‑GACCGCTGCTAGC‑GC‑GGTCAtt < 5:194068/137‑1 (MQ=255) cACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCAGCAAT‑GACCGCTGCTAGC‑GC‑GGTCAtt > 6:194068/1‑137 (MQ=255) ttCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTaa < 7:329760/148‑1 (MQ=255) ttCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTaa < 6:162495/148‑1 (MQ=255) ccgatagagGTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑TGTCATTGCTCAGGTCCTCCGTAAGc < 8:610/138‑1 (MQ=255) cccAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGTGGCTGCTGTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGATCAGGTCCTCCGTAAGc > 7:610/1‑143 (MQ=255) ccAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGTCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGa < 8:183061/149‑1 (MQ=255) cAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGAc > 6:360816/1‑149 (MQ=255) aaaGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGAc > 5:180201/1‑148 (MQ=255) aaGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGActc < 7:303279/149‑1 (MQ=255) gCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCt < 4:3380/149‑1 (MQ=255) cgtgTTCTCTGGCTGATCTGACGGGCAGTACCGCGGACAATGCAGTGGCTGCTTTGGAGCCGTTGCGAGACACAAGACCTCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAATCATCCTGACTCTGCCGCtt < 4:348908/146‑1 (MQ=255) cGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGc < 2:255626/147‑1 (MQ=255) cGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCtt < 3:199167/149‑1 (MQ=255) gctcgctGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACAAAAGACCGCTCCTGCAAT‑GACCCC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGc > 8:151279/1‑147 (MQ=255) ctcgctGATCTGACGGGCAGTACCGCCGCCAATGCAGCGGCTGCTTTGCAGCCCTTGCGCGACACAACACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAACCAACCTCACTCTGCCGCTTGATCCCac > 8:291972/1‑148 (MQ=255) gctGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGca < 7:291972/148‑1 (MQ=255) cGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGatat > 6:140626/1‑149 (MQ=255) tACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGc < 6:180201/149‑1 (MQ=255) tACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCCGc > 4:176040/1‑149 (MQ=255) ccgccgCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGcaccac < 8:220725/133‑1 (MQ=255) ccgccgCCAATGCAGGGGCTGCTTGGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGcaccac > 7:220725/1‑133 (MQ=255) cgccgcCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGa > 2:302283/1‑107 (MQ=255) cgccgcCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGa < 1:302283/107‑1 (MQ=255) ccAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAgcg < 5:360816/149‑1 (MQ=255) aaTGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTGCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCCGCACCTGATATCGGCTGCGAGCCgcggc > 2:45645/1‑149 (MQ=255) aaTGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCt > 8:69807/1‑137 (MQ=255) aaTGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCt < 7:69807/137‑1 (MQ=255) aagggCTGCAAAGCAGCCCCTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGCTGGACATTGCTCAGGTCCTCCGTAAGCAACATGACTCTGCCGCTTGATGCCAGCACAACCTGATATCGGATGCGAGCAGCGGCGCGt > 1:286289/3‑149 (MQ=255) gggCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCg > 7:71173/1‑149 (MQ=255) tGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAATAGACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCACCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTc > 1:239654/1‑149 (MQ=255) gCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCgg > 6:225660/1‑118 (MQ=255) gCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCgg < 5:225660/118‑1 (MQ=255) gCAGCCCTAGCGCGACACAAGCCCGCTAATGCAAA‑GACCGC‑GCGAGCAGC‑GGTCATTGCTCAGGGCCTCCGTAACCAACCTGCCTCTCCCCCTAGATCCcaccac > 8:153745/1‑105 (MQ=255) cTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGc < 2:282972/134‑1 (MQ=255) cTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGc > 1:282972/1‑134 (MQ=255) cTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTg > 5:175718/1‑149 (MQ=255) ttGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCGTGATGCCAGCCCCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTgc > 7:168532/1‑149 (MQ=255) gcgcGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTgcg < 8:306998/148‑1 (MQ=255) gcgcGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTgc < 2:239654/147‑1 (MQ=255) acaAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTGCGCCCAAc > 2:274110/1‑147 (MQ=255) cGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTGCGCCCAACCAGCTGTTg > 2:164546/1‑149 (MQ=255) tCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTGCGCCCAACCAGCTGTTGccc < 8:71173/149‑1 (MQ=255) | ACTGGCGCTGAAGAGCTGGACGGGCGCGAAGAGAACATCACCTTTTCAATGCCCAAAGAGCTGCGGGTTCGCTCGCTGATCTGACGGGCAGTACCGCCGCCAATGCAGGGGCTGCTTTGCAGCCCTTGCGCGACACAAGACCGCTCCTGCAAT‑GACCGC‑GCTAGCAGC‑GGTCATTGCTCAGGTCCTCCGTAAGCAACCTGACTCTGCCGCTTGATGCCAGCACCACCTGATATCGGCTGCGAGCAGCGGCGCGTTCGCACATTTCCAGTTTTGCGCCCAACCAGCTGTTGCCC > NC_002947/714851‑715143 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |