Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 58,435 G→C 7.3% intergenic (‑346/+342) PP_5424 ← / ← PP_0050 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294758,4350GC 7.3% 168.5 / 4.4 55intergenic (‑346/+342)PP_5424/PP_0050hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (24/27);  new base C (2/2);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

CTCCGTGAGGCCGGCCATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTCCAGGCC  >  NC_002947/58291‑58582
                                                                                                                                                |                                                                                                                                                   
ctctGTGTGGTCGGCAATAGTAATGCTTCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGTGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTgg                                                                                                                                                 <  8:137627/149‑1 (MQ=255)
   cGTGAGGCCGGCCATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGtt                                                                                                                                               <  7:63101/148‑1 (MQ=255)
     tGAGGCCGGCCATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCct                                                                                                                                            >  8:118532/1‑149 (MQ=255)
         gccggccATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAACCAGCTTCCTCTgc                                                                                                                                        <  8:112737/149‑2 (MQ=255)
          ccggccATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAACCAGCTTCCTCTgct                                                                                                                                       <  6:37210/149‑3 (MQ=255)
          ccggccATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTCGGCAGCAGAGGAAGCTGGTTCCTCTGaa                                                                                                                                       <  3:330573/149‑1 (MQ=255)
             gccATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAagta                                                                                                                                    <  8:1176/149‑1 (MQ=255)
              ccATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAagtag                                                                                                                                   >  5:351785/1‑149 (MQ=255)
               cATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAagtagt                                                                                                                                  >  7:326204/1‑149 (MQ=255)
                aTCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTAACAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAagtagt                                                                                                                                  >  8:80100/1‑148 (MQ=255)
                       tGCATCAGATCTTGATTGACACGGGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGCGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGAcggcgg                                                                                                                          <  6:118627/149‑1 (MQ=255)
                         cATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCggg                                                                                                                         <  3:307101/148‑1 (MQ=255)
                                  ttgattgaCACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTg                                                                                                                                         >  6:22180/1‑123 (MQ=255)
                                  ttgattgaCACGCGTGACGCTTTGGATAGCCTTGCAATTGATGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCATCTTAAGTAGTGACGGCGGGAATTGCCAGt                                                                                                               >  2:62518/1‑149 (MQ=255)
                                         acacGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGa                                                                                                                                        <  3:320040/117‑1 (MQ=255)
                                         acacGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGa                                                                                                                                        >  4:320040/1‑117 (MQ=255)
                                          cacGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCt                                                                                                       >  4:62585/1‑149 (MQ=255)
                                                  ccGCGTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGCTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCaa                                                                                               <  6:122923/149‑1 (MQ=255)
                                                        tGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCg                                                                                          <  6:51829/148‑1 (MQ=255)
                                                          gATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGc                                                                                         >  6:311909/1‑147 (MQ=255)
                                                               ccTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGc                                                                                                                   >  1:275842/1‑116 (MQ=255)
                                                               ccTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGc                                                                                                                   <  2:275842/116‑1 (MQ=255)
                                                               ccTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCGGTCGGATTCTGAACCCAAGGTCGCCAGAc                                                                                    >  2:39342/1‑147 (MQ=255)
                                                                 ttGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAgg                                                                                >  4:64640/1‑149 (MQ=255)
                                                                   gCAATTGAGGGCTGCCACTGCGCATTTGTCGTGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGa                                                                               <  8:326204/148‑1 (MQ=255)
                                                                   gCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGa                                                                               <  5:311909/148‑1 (MQ=255)
                                                                     aaTTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTc                                                                                                              >  6:42047/1‑115 (MQ=255)
                                                                     aaTTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTc                                                                                                              <  5:42047/115‑1 (MQ=255)
                                                                     aaTTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGc                                                                             >  4:158839/1‑148 (MQ=255)
                                                                     aaTTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCt                                                                            <  3:64640/149‑1 (MQ=255)
                                                                         gAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTaaa                                                                         <  6:351785/148‑1 (MQ=255)
                                                                            ggCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCGGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGAt                                                                     <  1:39342/149‑1 (MQ=255)
                                                                              cTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCg                                                                   >  6:348056/1‑149 (MQ=255)
                                                                               tGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGc                                                                                         <  5:152824/126‑1 (MQ=255)
                                                                               tGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGc                                                                                         >  6:152824/1‑126 (MQ=255)
                                                                               tGCAACAGCGCATTTGTCGGGCCTTGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGc                                                                  >  8:150621/1‑149 (MQ=255)
                                                                                 cAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGAt                                                                     <  7:89870/144‑1 (MQ=255)
                                                                                 cAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGAt                                                                     >  8:89870/1‑144 (MQ=255)
                                                                                  aaCTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGc                                                               >  1:47112/1‑149 (MQ=255)
                                                                                    cTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGATGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAAGCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGa                                                             <  1:62518/149‑1 (MQ=255)
                                                                                    cTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGa                                                             <  7:80100/149‑1 (MQ=255)
                                                                                         cATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGactact                                                        >  5:102850/1‑149 (MQ=255)
                                                                                               tCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCAt                                                  <  3:158839/149‑1 (MQ=255)
                                                                                                    ccTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCt                                              >  3:214279/1‑148 (MQ=255)
                                                                                                        ggTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTActc                                                       <  6:315163/135‑1 (MQ=255)
                                                                                                        ggTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTActc                                                       >  5:315163/1‑135 (MQ=255)
                                                                                                                cATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGTGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCa                                 <  3:230883/149‑1 (MQ=255)
                                                                                                                 aTGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCAc                                <  3:62585/149‑1 (MQ=255)
                                                                                                                      cccccGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCgtc                           <  2:47112/149‑1 (MQ=255)
                                                                                                                          cGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGACCCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCgtcggtc                       >  2:276331/1‑149 (MQ=255)
                                                                                                                                      ttcagAGGAACCAGCTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCAAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTACTCATCCCTTACCGAAAAGTCACTCGTCGGTCGTTAACGGCCCt           >  6:254784/3‑149 (MQ=255)
                                                                                                                                       tcagAGGAACCAGCTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCAAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCtt          >  4:222832/2‑149 (MQ=255)
                                                                                                                                        cagAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGac                                                            <  2:354289/98‑1 (MQ=255)
                                                                                                                                        cagAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGac                                                            >  1:354289/1‑98 (MQ=255)
                                                                                                                                            ggAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTCCAg     <  4:214279/149‑1 (MQ=255)
                                                                                                                                                gCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTCCAGGcc  >  3:252996/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
CTCCGTGAGGCCGGCCATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCCAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTCCAGGCC  >  NC_002947/58291‑58582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑