Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 868,379 C→T 8.1% P403P (CCG→CCA)  mqo‑I ← malate:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947868,3790CT 8.1% 138.3 / 2.2 49P403P (CCG→CCA) mqo‑Imalate:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (21/24);  new base T (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

TGGCCTGCTCGGGGAAGCAGCGCTCGATCAGTTCCAGCATGATCGATACGGTTACCGAAGCACCCGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCA  >  NC_002947/868231‑868527
                                                                                                                                                    |                                                                                                                                                    
gtgCCTGCTCGGGGAAGCAGCGCTCGATCAGTTCCAGCATGATCGATACGGTTACCGAAGCACCCGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTc                                                                                                                                                      <  7:935/147‑1 (MQ=255)
 ggCCTGCTCGGGGAAGCAGCGCTCGATCAGTTCCAGCATGATCGATACGGTTACCGAAGCACCCGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTc                                                                                                                                                      <  3:330542/148‑1 (MQ=255)
 ggCCTGCTCGGGGAAGCAGCGCTCGATCAGTTCCAGCATGATCGATACGGTTACCGAAGCACCCGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTc                                                                                                                                                      <  6:10025/148‑1 (MQ=255)
     tGCTCGGGGAAGCAGCGCTCGATCAGTTCCAGCATGATCGATACGGTTACCGAAGCACCCGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGtc                                                                                                                                                 >  4:34639/1‑149 (MQ=255)
          ggggAAGCAGCGCGCGATCAGTTCCAGCATGATCGATACGGTTACCGAAGCACCCGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGtctttc                                                                                                                                             <  5:185479/148‑1 (MQ=255)
            ggAAGCAGCGCTCGTACAGTTCCAGCATGATCGATACGGTTACCGAAGCACCCGGCGATGCGCCGAGCAGTGCATGCAGGCTGCCTTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTtg                                                                                                                                          <  5:15700/149‑1 (MQ=255)
            ggAAGCAGCGCTCGATCAGTTCCAGCATGATCGATACGGTTACCGAAGCACCCGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTtg                                                                                                                                          <  7:170587/149‑1 (MQ=255)
               agcagcGCTCGATCAGTTCCAGCATGATCGATACGGTTACCGAAGCACCCGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTtgatg                                                                                                                                       <  5:216970/149‑1 (MQ=255)
                          aTCAGTTCCAGCATGATCGATACGGTTACCGAAGCACCCGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCAcgcg                                                                                                                            >  8:294563/1‑149 (MQ=255)
                                   aGCATGATCGATACGGTTACCGAAGCACCCGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGc                                                                                                                   <  7:245253/149‑1 (MQ=255)
                                            gATACGGTTACCGAAGCACCCGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAg                                                                                                          <  2:246317/149‑1 (MQ=255)
                                              tACGGTTACCGAAGCACCCGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCGGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGgc                                                                                                        >  1:110451/1‑149 (MQ=255)
                                                   ttACCGAAGCACCCGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACgcgc                                                                                                                           >  5:78159/1‑125 (MQ=255)
                                                   ttACCGAAGCACCCGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACgcgc                                                                                                                           <  6:78159/125‑1 (MQ=255)
                                                        gAAGCACCCGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTc                                                                                              >  3:49134/1‑149 (MQ=255)
                                                             aCCCGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCt                                                                                         >  4:231196/1‑149 (MQ=255)
                                                              cccGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTTGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCtt                                                                                        >  5:127436/1‑149 (MQ=255)
                                                              cccGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTTGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCtt                                                                                        >  5:133117/1‑149 (MQ=255)
                                                               ccGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTg                                                                                       <  7:294563/149‑1 (MQ=255)
                                                                cGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTgg                                                                                      <  4:49134/149‑1 (MQ=255)
                                                                  gCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGcc                                                                                    >  6:211932/1‑149 (MQ=255)
                                                                     aTGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCGGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGc                                                                                 <  2:110451/149‑1 (MQ=255)
                                                                        cgcCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCgg                                                                               <  3:231196/148‑1 (MQ=255)
                                                                          ccGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTTGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATa                                                                            <  6:133117/149‑1 (MQ=255)
                                                                          ccGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTTGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATa                                                                            <  6:127436/149‑1 (MQ=255)
                                                                          ccGAGCAGTGCAGCCACGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATa                                                                            <  5:279802/149‑1 (MQ=255)
                                                                             aGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGa                                                                          >  6:198934/1‑148 (MQ=255)
                                                                                    cAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGc                                                                  >  4:201458/1‑149 (MQ=255)
                                                                                       ccAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAgg                                                               <  5:198934/149‑1 (MQ=255)
                                                                                            cTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTc                                                          <  3:34639/149‑1 (MQ=255)
                                                                                              gCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTc                                                                                                 <  6:89932/108‑1 (MQ=255)
                                                                                              gCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTc                                                                                                 >  5:89932/1‑108 (MQ=255)
                                                                                                    ctcggCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTCCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCtt                                                           >  4:341955/4‑140 (MQ=255)
                                                                                                    cTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCtt                                                           <  3:341955/140‑1 (MQ=255)
                                                                                                    cTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACgc                                                   <  5:266387/148‑1 (MQ=255)
                                                                                                          cGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCa                                            >  4:238973/1‑149 (MQ=255)
                                                                                                                cAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACt                                      <  5:211932/149‑1 (MQ=255)
                                                                                                                 aGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTg                                     >  4:324794/1‑149 (MQ=255)
                                                                                                                        tACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCCCGCGCTGACACGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACt                              >  6:30628/1‑149 (MQ=255)
                                                                                                                         aCCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACtt                             >  6:47893/1‑149 (MQ=255)
                                                                                                                           cGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGc                                    <  7:126069/140‑1 (MQ=255)
                                                                                                                           cGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGc                                    >  8:126069/1‑140 (MQ=255)
                                                                                                                           cGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCg                           <  5:47893/149‑1 (MQ=255)
                                                                                                                           cGAACTGCAGAACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCAGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCg                           >  8:285733/1‑149 (MQ=255)
                                                                                                                            gAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGc                          >  3:84996/1‑149 (MQ=255)
                                                                                                                            gAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGc                          >  4:55981/1‑149 (MQ=255)
                                                                                                                            gAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGc                          >  8:161005/1‑149 (MQ=255)
                                                                                                                             aaCTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCt                         <  7:161005/149‑1 (MQ=255)
                                                                                                                                 gcagcaCGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGc                          <  3:296719/144‑1 (MQ=255)
                                                                                                                                 gcagcaCGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGc                          >  4:296719/1‑144 (MQ=255)
                                                                                                                                         ccgccTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACtt             <  3:55981/149‑1 (MQ=255)
                                                                                                                                          cgccTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTg            <  3:238973/149‑1 (MQ=255)
                                                                                                                                                    cGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTcc   <  3:201458/148‑1 (MQ=255)
                                                                                                                                                    cGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCATTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGCCCAGGTACTTGGTCAGGTCCa  >  4:40072/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGGCCTGCTCGGGGAAGCAGCGCTCGATCAGTTCCAGCATGATCGATACGGTTACCGAAGCACCCGGCGATGCGCCGAGCAGTGCAGCCAGGCTGCCGTCCTGGGCCGAGACCAGCTCGGTACCGAACTGCAGCACGCCGCCTTTTTTCGGGTCTTTCTTGATGATCTGCACGCGCTGACCCGCCACTTCCAGGCGCCAGTCCTCGGCCTTGGCCTGCGGATAGAAACGACGCAGGGCTTCCAGACGCTGCTCCATCGACTGCATCACTTCGCTGACCAGGTACTTGGTCAGGTCCA  >  NC_002947/868231‑868527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑