Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 871,873 C→T 9.0% intergenic (+19/+17) PP_0755 → / ← PP_0756 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947871,8730CT 9.0% 153.2 / 3.5 55intergenic (+19/+17)PP_0755/PP_0756hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (25/25);  new base T (2/3);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

TACATGGCAGTAGACGAGGGGGTAGTGGACGCTTTCGAACTTCGGGTGCCGAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGCTCTTGCGGGCAGGCATCCAGGCGCCGTTGCCATTCTTCCTTGGCCTTGGC  >  NC_002947/871729‑872021
                                                                                                                                                |                                                                                                                                                    
tACATGGCAGTAGACGAGGGGGTAGTGGACGCTTTCGAACTTCGGGTGCCGAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAg                                                                                                                                                  <  3:17428/149‑1 (MQ=255)
     ggCAGTAGACGAGGGGGTAGTGGACGCTTTCGAACTTCGGGTGCCGAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGcccc                                                                                                                                              >  1:193256/1‑148 (MQ=255)
      gCAGTAGACGAGGGGGTAGTGGACGCTTTCGAACTTCGGGTGCCGAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGccc                                                                                                                                               >  1:214930/1‑146 (MQ=255)
      gCAGTAGACGAGGGGGTAGTGGACGCTTTCGAACTTCGGGTGCCGAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGccc                                                                                                                                               <  2:214930/146‑1 (MQ=255)
       cAGTAGACGAGGGGGTAGTGGACGCTTTCGAACTTCGGGTGCCGAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATTGGGGCTGCGTTGCAGccccgat                                                                                                                                           <  4:31464/149‑4 (MQ=255)
             aCGAGGGGGTAGTGGACGCTTTCGAACTTCGGGTGCCGAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTGTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGAt                                                                                                                                     <  4:341592/149‑1 (MQ=255)
                      tAGTGGACGCTTTCGAACTTCGGGTGCCGAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTa                                                                                                                              >  3:267731/1‑147 (MQ=255)
                        gTGGACGCTTTCGAACTTCGGGTGCCGAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAgg                                                                                                                          >  3:63093/1‑149 (MQ=255)
                           gACGCTTTCGAACTTCGGGTGCCGAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACAATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGc                                                                                                                       <  7:54312/149‑1 (MQ=255)
                               cTTTCGAACTTCGGGTGCCGAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCctgc                                                                                                                   <  4:63093/149‑1 (MQ=255)
                                 ttCGAACTTCGGGTGCCGAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCAGCAGGCACctgtag                                                                                                                 >  1:306930/1‑146 (MQ=255)
                                       cTTCGGGTGCCGAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGcctgac                                                                                                           >  5:300962/1‑149 (MQ=255)
                                           gggTGCCGAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCAGCAGGCACCTGTAGcctgatcag                                                                                                        <  2:306930/148‑5 (MQ=255)
                                            ggTGTCGAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGcctgacctga                                                                                                       >  2:156616/1‑148 (MQ=255)
                                                  gAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACGTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGt                                                                                                >  8:5757/1‑149 (MQ=255)
                                                  gAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCGTTGCAGccccgat                                                                                                                                           <  5:291391/106‑4 (MQ=255)
                                                  gAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCGTTGCAGccccgat                                                                                                                                           >  6:291391/1‑103 (MQ=255)
                                                    aaTACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTg                                                                                              <  6:300962/149‑1 (MQ=255)
                                                    aaTACTTCTACATCCTGGGCTGTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTg                                                                                              <  4:24211/149‑1 (MQ=255)
                                                      gacgtCTACATGAAGGGCTTTGCGTGCGTGGTGATGGCGCTGCGGCTGTCCTGGTTCAGCAGCTGGGTCTGAGAACCTTCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGTCCTGATACTGGTTgcg                                                                                            <  8:161808/145‑1 (MQ=255)
                                                       aCTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTg                                                                                                  >  7:319246/1‑142 (MQ=255)
                                                       aCTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTg                                                                                                  <  8:319246/142‑1 (MQ=255)
                                                        cTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGccc                                                                                                                                               >  6:298198/1‑96 (MQ=255)
                                                        cTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGccc                                                                                                                                               <  5:298198/96‑1 (MQ=255)
                                                                  cTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCg                                                                                 >  1:13167/1‑148 (MQ=255)
                                                                   tGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAAc                                                                                                                                          <  3:313485/90‑1 (MQ=255)
                                                                   tGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAAc                                                                                                                                          >  4:313485/1‑90 (MQ=255)
                                                                    gggCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCa                                                                               >  3:103742/1‑148 (MQ=255)
                                                                            cGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGcctg                                                                                                             <  7:236255/110‑1 (MQ=255)
                                                                            cGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGcctg                                                                                                             >  8:236255/1‑110 (MQ=255)
                                                                               gCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAacac                                                                    >  2:332939/1‑148 (MQ=255)
                                                                                    gCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGt                                                               <  1:332939/148‑1 (MQ=255)
                                                                                      gATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACg                                                            <  2:193256/149‑1 (MQ=255)
                                                                                        tGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGc                                                          <  3:346229/149‑1 (MQ=255)
                                                                                         ggCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGcc                                                         >  7:333673/1‑149 (MQ=255)
                                                                                            gctgctgctGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCgct                                                      <  4:267731/149‑1 (MQ=255)
                                                                                                ctgctgTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGctc                                                  <  1:156616/149‑1 (MQ=255)
                                                                                                  gctgTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGCTCtt                                                >  4:121265/1‑149 (MQ=255)
                                                                                                   ctgTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGt                                                                                                <  7:75965/100‑1 (MQ=255)
                                                                                                   ctgTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGt                                                                                                >  8:75965/1‑100 (MQ=255)
                                                                                                               ccccgatGGTTCTCAGACCCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGAAACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGCTCTTGCGGGCAGGCATc                                   >  4:286030/7‑149 (MQ=255)
                                                                                                                    cTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGCTCTTGCGGGCAGGCATCCAGgc                              <  3:121265/149‑1 (MQ=255)
                                                                                                                        gTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGCTCTTGCGGGCAGGCATCCAGGCGCCg                          >  7:199115/1‑149 (MQ=255)
                                                                                                                        gTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCACGTACGGCCGCTGCTCTTGCGGGCAGGCATCCAGGCGCCg                          >  4:86873/1‑149 (MQ=255)
                                                                                                                         tCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGCTCTTGCGGGCAGGCATCCAGGCGCCGt                         <  4:103742/149‑1 (MQ=255)
                                                                                                                                  cATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGCTCTTGCGGGCAGGCATCCAGGCGCCGTTGCCAttct                >  8:148633/1‑149 (MQ=255)
                                                                                                                                  cATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGCTCTTGCGGGCAGGCATCCAGGCGCCGTTGCCAttct                >  3:273563/1‑149 (MQ=255)
                                                                                                                                   aTCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAg                                                                 <  2:204772/99‑1 (MQ=255)
                                                                                                                                    ttggggCTGCGTTGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGCTCTTGCGggca                                         <  4:298659/120‑1 (MQ=255)
                                                                                                                                    ttggggCTGCGTTGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGCTCTTGCGggca                                         >  3:298659/3‑122 (MQ=255)
                                                                                                                                      ggggCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGCTCTTGCGGGCAGGCATCCAGGCGCCGTTGCCATTCTTcc             <  7:5757/148‑1 (MQ=255)
                                                                                                                                             cAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGCTCTTGCGGGCAGGCATCCAGGCGCCGTTGCCATTCTTccttggcctt     >  6:181474/1‑149 (MQ=255)
                                                                                                                                             cAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGCTCTTGCGGGCAGGCATCCAGGCGCCGTTGCCATTCTTccttggcc       <  7:173958/147‑1 (MQ=255)
                                                                                                                                               aCGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGCTCTTGCGggcagg                                       >  4:89557/1‑113 (MQ=255)
                                                                                                                                               aCGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGCTCTTGCGggcagg                                       <  3:89557/113‑1 (MQ=255)
                                                                                                                                                cGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGCTCTTGCGGGCAGGCATCCAGGCGCCGTTGCCATTCTTCcttggccttggc  <  8:199115/149‑1 (MQ=255)
                                                                                                                                                cGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGCTCTTGCGGGCAGGCATCCAGGCGCCGTTGCCATTCTTCcttggccttggc  <  2:13167/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TACATGGCAGTAGACGAGGGGGTAGTGGACGCTTTCGAACTTCGGGTGCCGAAATACTTCTACATCCTGGGCTTTTCGTGCCTGGCGATGGCGCTGCTGCTGTCCTGGTTCAGCACCTGGGTCTGAGAACCATCGGGGCTGCAACGCAGCCCCAAACCTGATCAGGCTACAGGTGCCTGCTGCCTGACCTGATACTGGTTGCGCACCGGCGTCGCATACTGCAACACCAGGTACGGCCGCTGCTCTTGCGGGCAGGCATCCAGGCGCCGTTGCCATTCTTCCTTGGCCTTGGC  >  NC_002947/871729‑872021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑