Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 901,419 A→T 12.7% intergenic (‑138/‑193) cysZ ← / → trxB sulfate transport protein CysZ/thioredoxin reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947901,4190AT12.7% 138.9 / 10.1 55intergenic (‑138/‑193)cysZ/trxBsulfate transport protein CysZ/thioredoxin reductase
Reads supporting (aligned to +/- strand):  ref base A (23/25);  new base T (4/3);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

GGCTTGCATCTAGAGACTCCGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGTCCTTTTTAATTTCTTGGCTGGCGC  >  NC_002947/901273‑901562
                                                                                                                                                  |                                                                                                                                               
ggCTTGCATCTAGAGACTCCGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATg                                                                                                                                               >  6:39763/1‑149 (MQ=255)
      cATCTAGAGACTCCGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTAt                                                                                                                                          <  5:128865/148‑1 (MQ=255)
       aTCTAGAGACTCCGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTAt                                                                                                                                          >  3:326787/1‑147 (MQ=255)
            gagaCTCCGCAGAGAAGGAAACCGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCATCGCCTATgctg                                                                                                                                      <  3:118678/146‑4 (MQ=255)
            gagaCTCCGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGt                                                                                                                                     <  6:241378/147‑1 (MQ=255)
            gagaCTCCGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTgg                                                                                                                                   <  7:44170/149‑1 (MQ=255)
            gagaCTCCGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTg                                                                                                                                    >  8:44170/1‑148 (MQ=255)
            gagaCTCCGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCATCGCCTATgct                                                                                                                                       <  7:261500/145‑3 (MQ=255)
               aCTCCGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGAtt                                                                                                                                <  7:55881/149‑1 (MQ=255)
                 tCCGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGt                                                                                                                              >  5:90810/1‑149 (MQ=255)
                   cGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTc                                                                                                                             <  5:267761/148‑1 (MQ=255)
                     cAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTaa                                                                                                                          <  6:90810/149‑1 (MQ=255)
                      agagAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGTGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTaa                                                                                                                          <  8:211637/148‑1 (MQ=255)
                      agagAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCt                                                                                                                            >  7:211637/1‑146 (MQ=255)
                             gAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCGTATGAGGTGGATTGTCTAAGGATAttt                                                                                                                  >  7:163396/1‑149 (MQ=255)
                             gAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATAttt                                                                                                                  <  6:351212/149‑1 (MQ=255)
                             gAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATAttt                                                                                                                  >  4:140049/1‑149 (MQ=255)
                                 aCGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTg                                                                                                                                    >  6:173547/1‑127 (MQ=255)
                                 aCGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTg                                                                                                                                    <  5:173547/127‑1 (MQ=255)
                                         aCCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCttt                                                                                                       <  3:140049/148‑1 (MQ=255)
                                           cTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGc                                                                                                     <  1:231624/148‑1 (MQ=255)
                                           cTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGc                                                                                                     >  2:231624/1‑148 (MQ=255)
                                           cTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCg                                                                                                    >  5:169353/1‑149 (MQ=255)
                                                              cgacgaAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACACTGCGCt                                                                                  >  1:187191/1‑148 (MQ=255)
                                                                  gaAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACt                                                                             >  5:211333/1‑149 (MQ=255)
                                                                       gCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTc                                                                         >  3:106306/1‑148 (MQ=255)
                                                                           gCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCttt                                                                    >  7:241791/1‑149 (MQ=255)
                                                                              gTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCg                                                                 <  5:39763/149‑1 (MQ=255)
                                                                                     ggAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTgc                                                          <  8:241791/149‑1 (MQ=255)
                                                                                      gAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTTCAGTGCGCTCAACTTTTCGCTTTGCGGGCCTgcg                                                         <  8:212104/149‑1 (MQ=255)
                                                                                        aaCGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTc                                                                                                                             >  4:266636/1‑79 (MQ=255)
                                                                                        aaCGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTc                                                                                                                             <  3:266636/79‑1 (MQ=255)
                                                                                           gggATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACACTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGttt                                                    <  2:187191/149‑1 (MQ=255)
                                                                                                  aaCAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCttt                                                                    <  1:78447/126‑1 (MQ=255)
                                                                                                  aaCAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCttt                                                                    >  2:78447/1‑126 (MQ=255)
                                                                                                  aaCAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCttt                                                                    >  2:73453/1‑126 (MQ=255)
                                                                                                  aaCAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCttt                                                                    <  1:73453/126‑1 (MQ=255)
                                                                                                  aaCAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACATCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTGTTAGCCtt                                             >  6:174559/1‑149 (MQ=255)
                                                                                                       aaGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTcccc                                         >  1:330593/1‑148 (MQ=255)
                                                                                                             ccGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGtgct                                  <  2:330593/149‑1 (MQ=255)
                                                                                                                    ttGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTggg                           <  4:326787/149‑1 (MQ=255)
                                                                                                                        ttATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGTCCt                       >  3:17032/1‑149 (MQ=255)
                                                                                                                              aTGAAATCGCAGCATACGCGATGCCTATGAGGTGGAATGTCTAAGGATAATTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACATTTAGCTTTGCGGGCCTGCGCGTTTTAGCCTTCACCAAGTGCTCCGTGGGTCCTTTTTaa                 >  1:49914/1‑149 (MQ=255)
                                                                                                                                   aTCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGTCCTTTTTAATTTCt            >  4:33430/1‑149 (MQ=255)
                                                                                                                                      gcagcaTAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGTCCTTTTTAATTTCTtgg         <  6:169353/149‑1 (MQ=255)
                                                                                                                                       cctcaTAGGCATCGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTc                                            >  2:196241/4‑113 (MQ=255)
                                                                                                                                       cctcaTAGGCATCGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTc                                            <  1:196241/110‑1 (MQ=255)
                                                                                                                                        ctcaTAGGCATCGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGTCCTTTTTAATTTCTtggct       >  1:348936/3‑149 (MQ=255)
                                                                                                                                        agcaTAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGTCCTTTTTAATTTCTtggct       <  6:211333/149‑1 (MQ=255)
                                                                                                                                         tcaTAGGCATCGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGTCCTTTTTAATTTCTtggctg      >  6:234495/2‑149 (MQ=255)
                                                                                                                                          caTAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGTCCTTTTTAATTTCTtggc        >  2:344944/1‑146 (MQ=255)
                                                                                                                                          caTAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGTCCTTTTTAATTTCTtggc        <  1:344944/146‑1 (MQ=255)
                                                                                                                                          caTAGGCATCGCCTATGAGGTGGATTGTCTATGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGTCCTTTTTAATTTCTtggatgg     >  3:197960/1‑149 (MQ=255)
                                                                                                                                           aTAGGCTATGCCTATGATGTGGATTGTCTAAGGATATTTCCTTAATCTTTGGGACCTGGCTACTGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGTCCTTTTTAATTTCTtggctggc    <  2:49914/149‑1 (MQ=255)
                                                                                                                                              ggCGATGCCTATGAGGTGGATTGTCTAACGATATTTCCTTAATCTTTGCGACCTCGCTACACTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGGCCTTTTTAATTTCTTGGCGGgcgc  <  4:316797/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GGCTTGCATCTAGAGACTCCGCAGAGAAGGAAAACGCGCTGACCTTACCGAGTTTGCCGCGCCGACGAAAGGCACGCCGTGGGCGGGAAACGGGATGTAACAAAAGCGCCCGGGGCTTGTTTATCGATGAAATCGCAGCATAGGCGATGCCTATGAGGTGGATTGTCTAAGGATATTTCCTTAATCTTTGCGACCTCGCTACAGTGCGCTCAACTTTTCGCTTTGCGGGCCTGCGCGTTTTAGCCTTCCCCAAGTGCTGCGTGGGTCCTTTTTAATTTCTTGGCTGGCGC  >  NC_002947/901273‑901562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑