Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 931,231 C→G 6.9% L4797L (CTG→CTC)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947931,2310CG 6.9% 175.0 / 3.8 58L4797L (CTG→CTC) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (24/30);  new base G (2/2);  total (26/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.37e-01

GGATCGGCCTGCAGTACCGACAGTGTCTGGTTGTTGATTTGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTG  >  NC_002947/931085‑931375
                                                                                                                                                  |                                                                                                                                                
ggATCGGCCTGCAGTACCGACAGTGTCTGGTTGTTGATTTGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAg                                                                                                                                                >  4:180033/1‑149 (MQ=255)
  aTCGGCCTGCAGTACCGACAGTGTCTGGTTGTTGATTTGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTGGGCGAGGc                                                                                                                                              <  8:186957/149‑1 (MQ=255)
   tCGGCCTGCAGTACCGACAGTGTCTGGTTGTTGATTTGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGcc                                                                                                                                             >  4:210278/1‑149 (MQ=255)
     ggCCTGCAGTACCGACAGTGTCTGGTTGTTGATTTGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGc                                                                                                                                           >  2:44697/1‑149 (MQ=255)
       ccTGCAGTACCGACAGTGTCTGGTTGTTGATTTGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATGAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTg                                                                                                                                         <  1:321777/149‑1 (MQ=255)
          gCAGTACCGACAGTGTCTGGTTGTTGATTTGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAgg                                                                                                                                               <  1:230906/140‑1 (MQ=255)
          gCAGTACCGACAGTGTCTGGTTGTTGATTTGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAgg                                                                                                                                               >  2:230906/1‑140 (MQ=255)
           cAGTACCGACAGTGTCTGGTTGTTGATTTGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCa                                                                                                                                     <  4:191980/149‑1 (MQ=255)
                ccGACAGTGTCTGGTTGTTGATTTGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTGGGCGAGGCCggc                                                                                                                                          >  7:112302/1‑137 (MQ=255)
                ccGACAGTGTCTGGTTGTTGATTTGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTGGGCGAGGCCggc                                                                                                                                          <  8:112302/139‑3 (MQ=255)
                    cAGTGTCTGGTTGTTGATTTGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGt                                                                                                                            <  3:180033/149‑1 (MQ=255)
                             gttgttGATTTGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGt                                                                                                                      <  7:29245/146‑1 (MQ=255)
                             gttgttGATTTGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGa                                                                                                                   <  5:328612/149‑1 (MQ=255)
                                       tgtgCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACtt                                                                                                          <  7:8689/148‑1 (MQ=255)
                                       tgtgCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACtt                                                                                                          <  4:118389/148‑1 (MQ=255)
                                         tgCGGGTGACAGTTCGACGCTGAAGCGGCCATCAGCCAGTACGCTACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTgg                                                                                                        <  2:327006/148‑1 (MQ=255)
                                             ggTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAg                                                                                                   <  1:44697/149‑1 (MQ=255)
                                              gTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGt                                                                                                  <  5:336939/149‑1 (MQ=255)
                                                gACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTgg                                                                                                >  1:64538/1‑149 (MQ=255)
                                                     ttCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTcgg                                                                                           <  5:119101/149‑1 (MQ=255)
                                                      tCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTcggg                                                                                          >  5:43904/1‑149 (MQ=255)
                                                        gACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTcgggc                                                                                         <  3:210278/148‑1 (MQ=255)
                                                               aaGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGaa                                                                                 <  3:96931/149‑1 (MQ=255)
                                                               aaGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGaa                                                                                 <  1:335255/149‑1 (MQ=255)
                                                                     ccATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCgg                                                                           <  5:161757/149‑1 (MQ=255)
                                                                     ccATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCg                                                                            <  6:43904/148‑1 (MQ=255)
                                                                       aTCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCgggg                                                                         >  4:109719/1‑149 (MQ=255)
                                                                        tCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGt                                                                        >  3:332287/1‑149 (MQ=255)
                                                                        tCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGt                                                                        <  2:64538/149‑1 (MQ=255)
                                                                              aGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAg                                                                  <  4:332287/149‑1 (MQ=255)
                                                                                   gATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAg                                                             >  2:128875/1‑149 (MQ=255)
                                                                                      aCCCGTGCCCAGTACGGTACCGGCCGCGATACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCa                                                          >  7:141797/1‑149 (MQ=255)
                                                                                          gTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCggg                                                                          <  1:246302/129‑1 (MQ=255)
                                                                                          gTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCggg                                                                          >  2:246302/1‑129 (MQ=255)
                                                                                          gTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGc                                                      <  5:332889/149‑1 (MQ=255)
                                                                                          gTGCCCAGGACGGTACCGGCCGCGGTACTCCCGATAACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGc                                                      <  8:161645/149‑1 (MQ=255)
                                                                                           tGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGcc                                                     <  3:109719/149‑1 (MQ=255)
                                                                                              ccAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGa                                                   >  8:272210/1‑148 (MQ=255)
                                                                                               cAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGaca                                                 >  2:241199/1‑149 (MQ=255)
                                                                                                    cGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCGGGGGTTTGCAGGGTAGCCACTGCCGACACGtt                                             <  1:128875/148‑1 (MQ=255)
                                                                                                     ggTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTgc                                           >  8:107431/1‑149 (MQ=255)
                                                                                                                  ttACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGc                              <  7:107431/149‑1 (MQ=255)
                                                                                                                      tGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCCCTGCCGACACGTTGCCGGCCGCATCGGCCTgg                          >  8:183459/1‑149 (MQ=255)
                                                                                                                      tGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTgg                          >  1:345634/1‑149 (MQ=255)
                                                                                                                        aCGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGt                        >  7:32577/1‑149 (MQ=255)
                                                                                                                           aTTACCGTGGCGCGGGCCTGGCCCAGGCCGCTGTCCACGCTGCCGTCGGCGGTGAACACCACTTGGGTGAGTGGGGCGGGCGGGGGGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGaa                     <  8:141797/149‑1 (MQ=255)
                                                                                                                           aTTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTg                       >  5:129190/1‑147 (MQ=255)
                                                                                                                             tACCGTGGCCCCGGCCTCGCCCAGGCCGCTGACCACGCTGACGTCGGCGATGATCACCACTTGGGTGAGTGGGTCGGGCGGGGAGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCCACACGTTCCCGTCAGCATAGGCCTGGGTGAAGc                   >  6:89509/1‑149 (MQ=255)
                                                                                                                               ccGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTcgggcggg                                                                                      <  5:242085/80‑1 (MQ=255)
                                                                                                                               ccGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTAGGCGTTGATCACCACTTGGGTGAGTGGGTcgggcggg                                                                                      >  6:242085/1‑80 (MQ=255)
                                                                                                                                  ggtcagcGGCCTGGGCGAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTggg                         >  7:317462/7‑138 (MQ=39)
                                                                                                                                     cgcCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCC‑GGGTTTGCAGGGTAGCCCCTGCCGACACGTTGCCGGCCGCCTCGGCCTGGGTGAAGCTGCGCAGCt          >  4:286813/1‑149 (MQ=255)
                                                                                                                                      gcCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTg                           <  1:81972/132‑1 (MQ=255)
                                                                                                                                      gcCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTg                           >  2:81972/1‑132 (MQ=255)
                                                                                                                                        cGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCg        <  1:241199/149‑1 (MQ=255)
                                                                                                                                            cTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGt    >  7:297832/1‑149 (MQ=255)
                                                                                                                                              cGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGCTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTg  <  1:103768/149‑1 (MQ=255)
                                                                                                                                               gCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTg  >  2:103768/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GGATCGGCCTGCAGTACCGACAGTGTCTGGTTGTTGATTTGTGCGGGTGACAGTTCGACGCTGAAGCTGCCATCAGCCAGTACGATACCCGTGCCCAGTACGGTACCGGCCGCGTTACTGACGATTACCGTGGCGCCGGCCTCGCCCAGGCCGCTGACCACGCTGCCGTCGGCGTTGATCACCACTTGGGTGAGTGGGTCGGGCGGGGTGAAGTCCGGGGTTTGCAGGGTAGCCACTGCCGACACGTTGCCGGCCGCATCGGCCTGGGTGAAGCTGAGCAGCTCGGCGTTG  >  NC_002947/931085‑931375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑