Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	932,607	T→C	6.6%	N4339D (AAC→GAC)	PP_0806 ←	surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	932,607	0	T	C	6.6%	171.6 / 2.3	60	N4339D (AAC→GAC)	PP_0806	surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base T (27/29);  new base C (2/2);  total (29/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

ACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGCACGGTCACGG  >  NC_002947/932464‑932744                                                                                                                                                |                                                                                                                                          aCCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCgg                                                                                                                                      >  4:236712/1‑149 (MQ=255)  ccGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCgg                                                                                                                                      <  3:98804/148‑1 (MQ=255)    gTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCAACGTGTCGCCGGccg                                                                                                                                   <  2:205273/149‑3 (MQ=255)    gTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCAACGTGTCGCCGGcc                                                                                                                                    >  1:210528/1‑147 (MQ=255)    gTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCAACGTGTCGCCGGcc                                                                                                                                    <  2:210528/148‑2 (MQ=255)     tCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGc                                                                                                                                  <  3:236712/149‑1 (MQ=255)         gTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGACTGCGTCg                                                                                                                              <  2:71884/149‑1 (MQ=255)            cTCGTACCGTCTCTGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGTCTGCCAGGTTGGCAACTGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGt                                                                                                                            <  3:101821/148‑1 (MQ=255)             tCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTgg                                                                                                                          >  8:330198/1‑149 (MQ=255)               gTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCg                                                                                                                        <  1:92248/149‑1 (MQ=255)                 aCCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGt                                                                                                                      >  6:97245/1‑149 (MQ=255)                  ccGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGt                                                                                                                      >  1:173947/1‑148 (MQ=255)                   cGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCa                                                                                                                    >  1:240164/1‑149 (MQ=255)                         ggTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCa                                                                                                              <  2:240164/149‑1 (MQ=255)                         ggTTGCGCCTGCTTCGCCATCACCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCa                                                                                                              <  2:212607/149‑1 (MQ=255)                          gTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGc                                                                                                                                  <  7:54578/128‑1 (MQ=255)                          gTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGc                                                                                                                                  >  8:54578/1‑128 (MQ=255)                               gcCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCt                                                                                                        >  5:36443/1‑149 (MQ=255)                                ccTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTg                                                                                                       <  4:58638/149‑1 (MQ=255)                                 cTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTgg                                                                                                      <  3:340461/149‑1 (MQ=255)                                  tGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGtt                                                                                                                                           <  6:197238/111‑1 (MQ=255)                                  tGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGtt                                                                                                                                           >  5:197238/1‑111 (MQ=255)                                    cTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGc                                                                                                     <  6:130382/147‑1 (MQ=255)                                        gCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTgg                                                                                               >  2:246994/1‑149 (MQ=255)                                         ccATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGt                                                                                              <  7:330198/149‑1 (MQ=255)                                          cATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGGCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTc                                                                                             >  1:252944/1‑149 (MQ=255)                                          cATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTg                                                                                                                >  8:260572/1‑130 (MQ=255)                                          cATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTg                                                                                                                <  7:260572/130‑1 (MQ=255)                                               ccTGTCAGGGTGGCGCCATTGGCGGAGAAAGCCAGGTTGGCCACCGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGAGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTCGTCAggg                                                                                         >  8:43716/1‑148 (MQ=255)                                                     aGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGtt                                                                                  >  5:290553/1‑149 (MQ=255)                                                                 ttGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTg                                                                      >  5:65558/1‑149 (MQ=255)                                                                    gCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGaa                                                                   <  2:64198/149‑1 (MQ=255)                                                                      ggAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTg                                                                                                       <  6:144594/111‑1 (MQ=255)                                                                      ggAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTg                                                                                                       >  5:144594/1‑111 (MQ=255)                                                                             gCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGtt                                                          <  5:10064/149‑1 (MQ=255)                                                                             gCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGtt                                                          <  6:290553/149‑1 (MQ=255)                                                                               cAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGc                                                              >  5:46967/1‑143 (MQ=255)                                                                               cAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGc                                                              <  6:46967/143‑1 (MQ=255)                                                                               cAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGc                                                        <  1:246994/149‑1 (MQ=255)                                                                                     ggCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCgg                                                  >  8:63556/1‑149 (MQ=255)                                                                                       ccACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGc                                                 >  7:216060/1‑148 (MQ=255)                                                                                        cACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTgg                                                                                          <  3:16015/106‑1 (MQ=255)                                                                                        cACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTa                                               >  6:356863/1‑149 (MQ=255)                                                                                        cACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTAGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTgg                                                                                          >  4:16015/1‑106 (MQ=255)                                                                                            gcggccggcggcGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGc                                           >  6:196125/1‑149 (MQ=255)                                                                                               gccggcggcGTTGTGTCCGCTGCTGTGTAAGGTGCGGCGGGCGACAGGTTGCCGGCTGGGTCTGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCtgt                                        <  7:43716/149‑1 (MQ=255)                                                                                                      gcgTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGc                                 <  5:356863/149‑1 (MQ=255)                                                                                                          ggtgTCCGCTGCGGTGTAAGGTGAGGCCGGCGACAAGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTTGTCTGGGCACTGTTCAAGGTGACCTGGAAGTTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGt                             <  6:58521/149‑1 (MQ=255)                                                                                                           gtgtCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGtt                            >  3:10932/1‑149 (MQ=255)                                                                                                                 gCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCt                      <  2:252944/149‑1 (MQ=255)                                                                                                                   tGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGaa                                                                   <  2:102019/102‑1 (MQ=255)                                                                                                                   tGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGaa                                                                   >  1:102019/1‑102 (MQ=255)                                                                                                                      ggTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCAGTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCAt                 >  4:92914/1‑149 (MQ=255)                                                                                                                       gtgtAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATc                >  1:152623/1‑149 (MQ=255)                                                                                                                       gtgtAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGGTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATc                >  7:318619/1‑149 (MQ=255)                                                                                                                        tgtAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATcg               >  8:129338/1‑149 (MQ=255)                                                                                                                          tAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATcgc              <  6:65558/148‑1 (MQ=255)                                                                                                                             ggTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcac          <  2:173947/149‑1 (MQ=255)                                                                                                                                  ggccggcGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacggtca     >  3:236292/1‑149 (MQ=255)                                                                                                                                  agccggcAACGTGTCGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacggtca     >  2:239263/2‑149 (MQ=255)                                                                                                                                    ccggcGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacggtcacg   <  5:97245/149‑1 (MQ=255)                                                                                                                                     cggcGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGcacggtcacgg  <  4:10932/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                          ACCGTCCGGTGCTCGTACCGTCACGGTTGCGCCTGCTTCGCCATCGCCTGTCAGGGTGGCGCCATTGGCGGAGACAGCCAGGTTGGCCACGGCGGCCGGCGGCGTGGTGTCCGCTGCGGTGTAAGGTGCGGCCGGCGACACGTTGCCGGCTGCGTCGGTGGCGGTCACCTGCAGTATCTGGCCATTGGTCTGGGCACTGTTCAAGGTGACCTGGAAGGTGCCGTTGCCACCGGCTACCGCTGTGCCCAGCACGTTGCCGCTGGCATCGCGCACGGTCACGG  >  NC_002947/932464‑932744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑