| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 58,747 | T→A | 16.3% | intergenic (‑658/+30) | PP_5424 ← / ← PP_0050 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 58,747 | 0 | T | A | 16.3% | 144.5 / 12.5 | 59 | intergenic (‑658/+30) | PP_5424/PP_0050 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (24/25); new base A (4/6); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGGCGC > NC_002947/58606‑58888 | tAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCgg < 2:73791/149‑1 (MQ=255)tAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCAGCAGCgg > 8:40639/1‑149 (MQ=255) aGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATATCACAA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCgg < 1:276331/148‑1 (MQ=255) aTTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCttt > 1:212402/1‑149 (MQ=255) tttCTCGGCTTTTCTGTTAAAAGCACCCCTTCATTACACAA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTTTTTTGTGAACCTACAAGGTGTAAATCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCtttt < 1:102298/149‑1 (MQ=255) tctcGGCTTTTCTGTTAAAAGCACCCCTTCATTTCAAAATAAAAAATGGCGAAGATTCGAATTTCACGGGGTCTGGCCTATCACCTATTTTGTTAACCTACAAGGTTTAATTCGCCAGACACTAAAAAGCCAGCTGCTGCTGGCtttt < 6:16673/148‑1 (MQ=255) ctcGTCTTTTATGTGAAAAGCACCCATTCATTTCACAT‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCAGCACCGGGCTTTTTa < 5:76690/148‑1 (MQ=255) ctcGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCAGCAGCGGGCTTTTTa < 1:44699/148‑1 (MQ=255) tcGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGGCACTAAAAAGCCCGCAGCAGCGGGCTTTTTa < 6:120271/147‑1 (MQ=255) tcGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGGCACTAAAAAGCCCGCAGCAGCGGGCTTTTTa > 5:120271/1‑147 (MQ=255) tcGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGt > 4:266650/1‑149 (MQ=255) ggCTTTTCTGTGAAAAGCACCCCTTCATTTCACAA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGt < 3:266650/147‑1 (MQ=255) ggCTTTTCTGTGAAAAGCACCCCTTCATTTCACAA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGc > 4:304113/1‑149 (MQ=255) tCTGTGAAAAGCACCCCTTCATTTCACAA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGTCTggc < 7:40639/148‑3 (MQ=255) tgAAAAGCACCCCTTCATTTCACAA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGTCTggcgaa < 1:299850/147‑6 (MQ=255) aaaaGCACCCCTTCATTTCACAA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATc < 2:247085/149‑1 (MQ=255) gCACCCCTTCATTTCACAA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTa < 2:212402/149‑1 (MQ=255) cccTTCATTTCACAA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCg > 3:320277/1‑149 (MQ=255) acaA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGtt < 3:304113/149‑1 (MQ=255) caA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGtt > 5:256142/1‑148 (MQ=255) aaaTGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATc < 6:256142/149‑1 (MQ=255) ccAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCaa < 3:10333/148‑1 (MQ=255) cAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATg > 5:97345/1‑149 (MQ=255) tttCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGcc < 6:97345/149‑1 (MQ=255) cACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCCGTCAGAATGTTCACGATCACACCGCTGCCAATGGccac > 8:242519/1‑148 (MQ=255) gggTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGaa > 5:62258/1‑149 (MQ=255) gTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGAt > 3:94424/1‑121 (MQ=255) gTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGAt < 4:94424/121‑1 (MQ=255) tGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTga > 3:215907/1‑105 (MQ=255) tGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTga < 4:215907/105‑1 (MQ=255) tGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAg > 4:163573/1‑149 (MQ=255) tATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGc > 8:179001/1‑148 (MQ=255) aCCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCAACAAGAACATAGTCGCCAtt > 4:198919/1‑148 (MQ=255) cTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACga > 2:135865/1‑149 (MQ=255) aCAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACgat > 6:154991/1‑148 (MQ=255) aCAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCAGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACgat > 2:195868/1‑148 (MQ=255) aaGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACgatgat < 4:320277/149‑1 (MQ=255) aGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTTCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCTGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCTCCATTCACATACAGCCAACGATGATc < 3:198919/149‑1 (MQ=255) ggTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTCAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCa > 4:250153/1‑149 (MQ=255) gCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGgc < 3:163573/148‑1 (MQ=255) ccAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGc > 7:182902/1‑85 (MQ=255) ccAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGc > 1:132541/1‑85 (MQ=255) ccAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGc < 8:182902/85‑1 (MQ=255) ccAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGc < 2:132541/85‑1 (MQ=255) ccAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGgcgc < 7:179001/149‑1 (MQ=255) ccAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGgcg > 7:107235/1‑148 (MQ=255) cAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCa < 6:62258/149‑1 (MQ=255) cacTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACa < 2:325295/119‑1 (MQ=255) cacTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACa > 1:325295/1‑119 (MQ=255) tAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGt > 2:39252/1‑149 (MQ=255) taaaaGCCCGCAGCAGCGGGCTTTTTAGTGTCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTg < 7:139488/148‑1 (MQ=255) aaatGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACAGCGCTTGCTATGGCCACCAGAACATAGTCGCCATTCACTTACAGCCAACGATGATCACGAGGTGGCGCATACAGGTg < 7:242519/148‑1 (MQ=255) aaaGCCCGCTGCTGGGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGgc > 7:304216/1‑149 (MQ=255) aaaGCCCGCTGCTGCTGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCTATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGgc < 2:274020/149‑1 (MQ=255) aaaGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAg > 3:189123/1‑114 (MQ=255) aaaGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAg < 4:189123/114‑1 (MQ=255) aaaGCACGCAGCAGCGGGCTTTTTAGTGTCTGCAAAAAATCAGTAGCCGGTGAGAATGTTCACGAAAAAACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATAAAGCCAACAATGATCACGACGTGGCGCATACaagatga > 8:139488/1‑143 (MQ=255) aaGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGgcg > 4:305739/1‑149 (MQ=255) aGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGgcgc < 8:107235/149‑1 (MQ=255) | TAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAA‑AAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGGCGC > NC_002947/58606‑58888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |