| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 955,155 | C→T | 30.1% | intergenic (+109/‑204) | cyoE → / → alaC | protoheme IX farnesyltransferase/aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 955,155 | 0 | C | T | 30.1% | 30.9 / 12.9 | 25 | intergenic (+109/‑204) | cyoE/alaC | protoheme IX farnesyltransferase/aminotransferase |
| Reads supporting (aligned to +/- strand): ref base C (7/10); new base T (2/5); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01 | |||||||||||
CAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGG‑AGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAT > NC_002947/955008‑955293 | cAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCt < 7:186293/149‑1 (MQ=255) cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGG‑TTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGG‑AGCgg < 3:26348/148‑1 (MQ=255) ggATGTGGTGATGAACTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGTAACCTTGTAGG‑AGCggg < 6:220789/149‑1 (MQ=37) ggATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGG‑AGCggg < 1:181514/149‑1 (MQ=37) ggATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTT‑CTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGG‑AGCGGGt < 6:302048/149‑1 (MQ=25) ggAGGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGGGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGG‑AGCgg < 8:153708/148‑1 (MQ=255) gATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGG‑AGCGGGt < 3:139308/149‑1 (MQ=255) atgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTTAAACCCCGGCAACAAGGTTGGGGTAGGCGGCGGTTTTAcccc > 3:34063/1‑147 (MQ=255) gcagtcTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGGCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGT‑AGCgg < 4:246182/124‑1 (MQ=25) gcacgcTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGG‑AGCgg > 3:246182/1‑129 (MQ=37) aaaCTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGG‑AGCGGGTTTa < 8:178800/126‑1 (MQ=255) aaaCTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGG‑AGCGGGTTTa > 7:178800/1‑126 (MQ=255) ctgcCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGG‑AGCgg < 4:316151/115‑1 (MQ=21) ctgcCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACAGGAACCTTGTAGG‑AGCgg > 3:316151/1‑115 (MQ=21) ggTAAACCCGCTCCTACAAGGTTCCTGTAGG‑AGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATaaaa < 7:164514/115‑1 (MQ=255) ggTAAACCCGCTCCTACAAGGTTCCTGTAGG‑AGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATaaaa > 8:164514/1‑115 (MQ=255) cccGCTCCTACAAGGTTCCTGTAGG‑AGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCgg < 4:319960/143‑1 (MQ=255) cccGCTCCTACAAGGTTCCTGTAGG‑AGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCgg > 3:319960/1‑143 (MQ=255) tCCTACAAGGTTCCTGTAGG‑AGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttct > 2:134580/1‑149 (MQ=255) cTACAAGGTTCCTGTAGG‑AGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTg < 1:134580/148‑1 (MQ=255) cTACAAGGTTCCTGTAGG‑AGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACATGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAATTTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGa > 8:67283/1‑149 (MQ=255) tACAAGGTTCCTGTAGG‑AGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttc < 7:253848/145‑1 (MQ=255) tACAAGGTTCCTGTAGG‑AGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtcttc > 8:253848/1‑145 (MQ=255) aCAAGGTTCCTGTAGG‑AGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAt > 4:183225/1‑148 (MQ=255) aCAAGGTTCCTGTAGG‑AGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAt < 3:183225/148‑1 (MQ=255) | CAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGTGCCGGGGTTTTTTTTTGGGGGTATTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCGCTCCTACAAGGTTCCTGTAGG‑AGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAAAGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGAT > NC_002947/955008‑955293 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |