| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 971,677 | C→T | 13.5% | intergenic (+32/‑32) | yajC → / → secD | protein translocase subunit YajC/protein translocase subunit SecD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 971,677 | 0 | C | T | 13.5% | 140.5 / 11.8 | 59 | intergenic (+32/‑32) | yajC/secD | protein translocase subunit YajC/protein translocase subunit SecD |
| Reads supporting (aligned to +/- strand): ref base C (27/24); new base T (6/2); total (33/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-02 | |||||||||||
CCGATGACTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTACAGATCAGCGGT > NC_002947/971542‑971825 | ccGATGACTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGCGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCcgc < 3:80728/148‑1 (MQ=255) aTGACTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCt < 8:100204/149‑1 (MQ=255) aCTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTg < 3:309672/148‑1 (MQ=255) cTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCtt > 3:228437/1‑146 (MQ=255) cTTCGTGGGGCTGGATGTGTCCGACACTGTCGAGCTGAAGTTCCAGATGGGTGCCATTGCGGCGACCCTGCAAAAAGGTACGCTCATGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCtt < 4:228437/146‑1 (MQ=255) gtgCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCAGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACgg > 1:236453/1‑145 (MQ=255) gtgCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCAGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACgg < 2:236453/145‑1 (MQ=255) gAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTg < 7:78988/147‑1 (MQ=255) gAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGAt > 8:163597/1‑149 (MQ=255) tgtCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCt > 5:361821/1‑148 (MQ=255) cGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAAc > 5:231620/1‑148 (MQ=255) cGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAAc > 4:117529/1‑148 (MQ=255) gACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCTCGCAACGCGACCCGCGTCTTGAACGGGCGGCGTGATGCTGAACa > 6:228875/1‑148 (MQ=255) cTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTGCGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAAAATCCTCtgtg > 7:238366/1‑149 (MQ=255) cTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCtgtg > 4:329132/1‑149 (MQ=255) gAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTgg > 1:11294/1‑148 (MQ=255) aaGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTg < 6:284427/123‑1 (MQ=255) aaGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTg > 5:284427/1‑123 (MQ=255) aaGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACaaa < 3:45580/135‑1 (MQ=255) aaGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACaaa > 4:45580/1‑135 (MQ=255) gAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCAc > 2:323949/1‑149 (MQ=255) gTGCCATTGCTGCGACCCTGCCAAAAGGTGCGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAAAATCCTCTGTGGAAATATGCACTGATc < 8:238366/149‑1 (MQ=255) tGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCg > 6:274190/1‑149 (MQ=255) ccATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTg < 3:117529/149‑1 (MQ=255) ttGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTg < 2:11294/149‑1 (MQ=255) tgcGACCCTGCCAAAAGGTACGCTCAAGGCTATGTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGaa > 4:293034/1‑108 (MQ=255) tgcGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACGGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGaa < 3:293034/108‑1 (MQ=255) tgcGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTAc > 5:104200/1‑149 (MQ=255) cccTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACtggtg > 2:65030/1‑149 (MQ=255) gCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCACCGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTAGTGGGGGTCg > 8:287749/1‑149 (MQ=255) ccAAACTGTACGGTCATGTCTAGTTGAGTTCCCGGTTTTTTTTTCCCGTCGGGGCCCGCACAGCGCCCCGCGGCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCg < 5:228875/148‑1 (MQ=255) ccAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCgg < 5:274190/149‑1 (MQ=255) cAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATACTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGt > 8:70523/1‑149 (MQ=255) aaacGGTACGCTCAAGTCTCTCTGAGTTACCGGTTTTATTTTCCAGTCGGGGAGCGCACCGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGtt < 6:104200/149‑1 (MQ=255) aaaGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGtt > 3:194335/1‑148 (MQ=255) gTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCct > 4:148487/1‑101 (MQ=255) gTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCct < 3:148487/101‑1 (MQ=255) gTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCAtt < 4:194335/149‑1 (MQ=255) cTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTc < 1:323949/149‑1 (MQ=255) tCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTcc > 7:261238/1‑149 (MQ=255) aGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCt < 7:163597/149‑1 (MQ=255) tCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCTTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCaa < 6:231620/148‑1 (MQ=255) tGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACct > 1:206332/1‑149 (MQ=255) ttACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTAc < 1:65030/149‑1 (MQ=255) ccGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACtgg > 3:151640/1‑111 (MQ=255) ccGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACtgg < 4:151640/111‑1 (MQ=255) ggTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGa > 1:52487/1‑149 (MQ=255) ttttATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGAt > 6:33215/1‑148 (MQ=255) ccAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTAtt > 7:62794/1‑116 (MQ=255) ccAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTAtt < 8:62794/116‑1 (MQ=255) cAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCcggcgg < 6:361821/148‑1 (MQ=255) tCGGGGCGCGTTGCGCGCCCCGCGTCTTGAACGGGCGGCGAGATGCAGAACAAATATCCTCTGTGGAAATAAGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTATACCCGGAAGACACGGCGGTa > 2:94031/1‑147 (MQ=255) gcGGGGCGCGTTGCGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTa > 6:152633/2‑147 (MQ=255) gcGGGGCGCGTTGCGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCAGCTCCCAACCTCTACCCGCATGACCCGGCGGTa > 2:98551/2‑147 (MQ=255) ggggCGCGTTGCGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACATATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTa > 8:163518/1‑145 (MQ=255) ggggCGCGTTGCGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACATATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTACAGa < 7:163518/149‑1 (MQ=255) ggggCGCGTTGCGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTa > 3:68310/1‑145 (MQ=255) ggggCGCGTTGCGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTACAg > 8:110826/1‑148 (MQ=255) gggCGCGTTGCGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTACAGAt < 5:152633/149‑1 (MQ=255) cAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTACAGATCAGCGGt < 8:261238/149‑1 (MQ=255) | CCGATGACTTCGTGGTGCTGGAAGTGTCCGACACTGTCGAGCTGAAGTTCCAGAAGGGTGCCATTGCTGCGACCCTGCCAAAAGGTACGCTCAAGGCTATCTGAGTTACCGGTTTTATTTTCCAGTCGGGGCGCGCAACGCGCCCCGCGTCTTGAACGGGCGGCGTGATGCTGAACAAATATCCTCTGTGGAAATATGCACTGATCGTGCTGGTACTGGTGGTCGGTTTCATTTATTCCGCTCCCAACCTCTACCCGGATGACCCGGCGGTACAGATCAGCGGT > NC_002947/971542‑971825 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |