Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,001,193 A→T 7.9% Q57L (CAG→CTG)  PP_0863 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,001,1930AT 7.9% 175.4 / 5.3 63Q57L (CAG→CTG) PP_0863hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (33/25);  new base T (3/2);  total (36/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.66e-01

GGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTACGCCCGT  >  NC_002947/1001047‑1001338
                                                                                                                                                  |                                                                                                                                                  
ggAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCcag                                                                                                                                                   >  5:285159/1‑148 (MQ=255)
     tggtggATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGAt                                                                                                                                             >  8:45738/1‑149 (MQ=255)
       gtggATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGc                                                                                                                                           >  2:353282/1‑149 (MQ=255)
               ggCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAgg                                                                                                                                               <  7:196388/137‑1 (MQ=255)
               ggCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAgg                                                                                                                                               >  8:196388/1‑137 (MQ=255)
               ggCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTg                                                                                                                                   >  7:289162/1‑149 (MQ=255)
                      ttGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCg                                                                                                                            >  6:28104/1‑149 (MQ=255)
                        gCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCg                                                                                                                            <  2:274404/147‑1 (MQ=255)
                              cATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCg                                                                                                                    >  1:182765/1‑149 (MQ=255)
                                     gAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGc                                                                                                             >  5:245719/1‑149 (MQ=255)
                                      aGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCt                                                                                                            <  7:277946/149‑1 (MQ=255)
                                             tCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAgg                                                                                                     >  8:147293/1‑149 (MQ=255)
                                                       gccgccCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCTGCTGGATGc                                                                                                                                           >  8:106094/1‑101 (MQ=255)
                                                       gccgccCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCTGCTGGATGc                                                                                                                                           <  7:106094/101‑1 (MQ=255)
                                                       gccgccCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCa                                                                                               >  8:26604/1‑145 (MQ=255)
                                                       gccgccCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCa                                                                                               <  7:26604/145‑1 (MQ=255)
                                                       gccgccCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCGGCTGGATGGGCGTGGCAGCCAGCAGGATGGCCCGGTTGCGAGGCGGTGGTTCGGTATCGCGGCCCAGGGTGGCAGTGc                                                                                           >  2:321845/1‑149 (MQ=255)
                                                           cccAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATg                                                                                       <  8:289162/149‑1 (MQ=255)
                                                              aGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAg                                                                                                      >  5:225274/1‑131 (MQ=255)
                                                              aGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAg                                                                                                      <  6:225274/131‑1 (MQ=255)
                                                               ggCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGgcgc                                                                                   <  7:147293/149‑1 (MQ=255)
                                                                      cTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCacaaca                                                                             <  2:182765/148‑1 (MQ=255)
                                                                       tGGCGGCAGCGGGGAAGGGTGTCGTCGAGTCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCAACGGTTGCCCGGCGTTGGTTCGGTATAGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATg                                                                           >  4:317556/1‑149 (MQ=255)
                                                                        ggcggcAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCATATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCgg                                                                                                                   >  1:85643/1‑108 (MQ=255)
                                                                        ggcggcAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTGGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGc                                                                          >  8:224821/1‑149 (MQ=255)
                                                                        ggcggcAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCgg                                                                                                                   <  2:85643/108‑1 (MQ=255)
                                                                        ggcggcAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATg                                                                                       <  6:347744/136‑1 (MQ=255)
                                                                        ggcggcAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATg                                                                                       >  5:347744/1‑136 (MQ=255)
                                                                            gcAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCgg                                                                      >  1:140096/1‑149 (MQ=255)
                                                                            gcAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCgg                                                                      <  7:45738/149‑1 (MQ=255)
                                                                                        ggTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTg                                                            >  8:255020/1‑147 (MQ=255)
                                                                                        ggTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTg                                                            >  2:325989/1‑147 (MQ=255)
                                                                                        ggTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGa                                                          <  5:28104/149‑1 (MQ=255)
                                                                                         gtgtGGGCGAGGCGCAGTTGCTGCTGGGGCAGAGCCTGCTGGAGGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAg                                                         <  6:47244/149‑1 (MQ=255)
                                                                                              gTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATgg                                                    >  8:170211/1‑149 (MQ=255)
                                                                                              gTCGAGGCGCACTTGCTGCTCGGGCAGATCCTGCTGGATGCGCGTCGCATCCACCAGGATGCCACCCTTGCCCGGCCTTGGTTCGGTATCGCTGCACACCTTGGCACTGCCAAAGCCCACAACATGCACCGGCCTTGACTGGAGCaacg                                                    >  2:346756/1‑146 (MQ=255)
                                                                                               tCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGCGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCa                                                                                               <  1:157368/105‑1 (MQ=255)
                                                                                               tCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGCGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCa                                                                                               >  2:157368/1‑105 (MQ=255)
                                                                                                cGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCacaac                                                                              >  1:99740/1‑121 (MQ=255)
                                                                                                cGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCacaac                                                                              <  2:99740/121‑1 (MQ=255)
                                                                                                 gAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTg                                                 <  7:170211/149‑1 (MQ=255)
                                                                                                      gcAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTg                                           >  3:202411/1‑149 (MQ=255)
                                                                                                         gTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCg                                        <  4:202411/149‑1 (MQ=255)
                                                                                                           tcatgctgTGGCAGATCCTGCTGGATGGGCGTGGCATCCAGAAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGa                                       <  4:155283/145‑1 (MQ=255)
                                                                                                                ctgGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGaa                                 >  3:339764/1‑149 (MQ=255)
                                                                                                                    ggCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTg                             <  7:255020/149‑1 (MQ=255)
                                                                                                                     gCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTgg                            <  6:285159/149‑1 (MQ=255)
                                                                                                                       agttgctgctgGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTGGGTATCGCTGGACAGGGTGGGAGTGCCTTGGCGCACAACATGGTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTg                          <  1:346756/144‑1 (MQ=255)
                                                                                                                          tCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGa                                                          <  4:240799/115‑1 (MQ=255)
                                                                                                                          tCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGa                                                          >  3:240799/1‑115 (MQ=255)
                                                                                                                           ccTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAgg                      >  6:254165/1‑149 (MQ=255)
                                                                                                                             tgctgGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATgg                                                    >  7:293338/1‑118 (MQ=255)
                                                                                                                             tgctgGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATgg                                                    <  8:293338/118‑1 (MQ=255)
                                                                                                                               ctgGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAggcggc                  >  6:139822/1‑149 (MQ=255)
                                                                                                                                      accGTGGCATCCTGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTg                                                 >  2:69558/3‑112 (MQ=255)
                                                                                                                                      accGTGGCATCCTGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTg                                                 <  1:69558/110‑1 (MQ=255)
                                                                                                                                       cgatGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGTGTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCAc           <  1:53506/146‑1 (MQ=255)
                                                                                                                                        cGTGGCATCCTGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTa         >  7:47912/1‑149 (MQ=255)
                                                                                                                                         gTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTAc        >  5:75604/1‑149 (MQ=255)
                                                                                                                                            gCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAggcggc                  >  5:55382/1‑136 (MQ=255)
                                                                                                                                            gCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAggcggc                  <  6:55382/136‑1 (MQ=255)
                                                                                                                                            gCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTACGcc     >  2:214588/1‑149 (MQ=255)
                                                                                                                                             cATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTACGccc    >  5:76156/1‑149 (MQ=255)
                                                                                                                                               tCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTACGCCCGt  >  8:216430/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTACGCCCGT  >  NC_002947/1001047‑1001338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑