Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,001,196 A→T 7.9% Q58L (CAG→CTG)  PP_0863 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,001,1960AT 7.9% 177.9 / 5.3 63Q58L (CAG→CTG) PP_0863hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (33/25);  new base T (3/2);  total (36/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-01

TGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTACGCCCGTGCGGCC  >  NC_002947/1001052‑1001344
                                                                                                                                                |                                                                                                                                                     
tggtggATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGAt                                                                                                                                                   >  8:45738/1‑149 (MQ=255)
  gtggATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGc                                                                                                                                                 >  2:353282/1‑149 (MQ=255)
          ggCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAgg                                                                                                                                                     <  7:196388/137‑1 (MQ=255)
          ggCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAgg                                                                                                                                                     >  8:196388/1‑137 (MQ=255)
          ggCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTg                                                                                                                                         >  7:289162/1‑149 (MQ=255)
                 ttGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCg                                                                                                                                  >  6:28104/1‑149 (MQ=255)
                   gCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCg                                                                                                                                  <  2:274404/147‑1 (MQ=255)
                         cATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCg                                                                                                                          >  1:182765/1‑149 (MQ=255)
                                gAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGc                                                                                                                   >  5:245719/1‑149 (MQ=255)
                                 aGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCt                                                                                                                  <  7:277946/149‑1 (MQ=255)
                                        tCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAgg                                                                                                           >  8:147293/1‑149 (MQ=255)
                                                  gccgccCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCTGCTGGATGc                                                                                                                                                 <  7:106094/101‑1 (MQ=255)
                                                  gccgccCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCTGCTGGATGc                                                                                                                                                 >  8:106094/1‑101 (MQ=255)
                                                  gccgccCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCa                                                                                                     >  8:26604/1‑145 (MQ=255)
                                                  gccgccCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCa                                                                                                     <  7:26604/145‑1 (MQ=255)
                                                  gccgccCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCGGCTGGATGGGCGTGGCAGCCAGCAGGATGGCCCGGTTGCGAGGCGGTGGTTCGGTATCGCGGCCCAGGGTGGCAGTGc                                                                                                 >  2:321845/1‑149 (MQ=255)
                                                      cccAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATg                                                                                             <  8:289162/149‑1 (MQ=255)
                                                         aGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAg                                                                                                            >  5:225274/1‑131 (MQ=255)
                                                         aGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAg                                                                                                            <  6:225274/131‑1 (MQ=255)
                                                          ggCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGgcgc                                                                                         <  7:147293/149‑1 (MQ=255)
                                                                 cTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCacaaca                                                                                   <  2:182765/148‑1 (MQ=255)
                                                                  tGGCGGCAGCGGGGAAGGGTGTCGTCGAGTCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCAACGGTTGCCCGGCGTTGGTTCGGTATAGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATg                                                                                 >  4:317556/1‑149 (MQ=255)
                                                                   ggcggcAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCATATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCgg                                                                                                                         >  1:85643/1‑108 (MQ=255)
                                                                   ggcggcAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTGGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGc                                                                                >  8:224821/1‑149 (MQ=255)
                                                                   ggcggcAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCgg                                                                                                                         <  2:85643/108‑1 (MQ=255)
                                                                   ggcggcAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATg                                                                                             <  6:347744/136‑1 (MQ=255)
                                                                   ggcggcAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATg                                                                                             >  5:347744/1‑136 (MQ=255)
                                                                       gcAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCgg                                                                            >  1:140096/1‑149 (MQ=255)
                                                                       gcAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCgg                                                                            <  7:45738/149‑1 (MQ=255)
                                                                                   ggTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTg                                                                  >  2:325989/1‑147 (MQ=255)
                                                                                   ggTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTg                                                                  >  8:255020/1‑147 (MQ=255)
                                                                                   ggTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGa                                                                <  5:28104/149‑1 (MQ=255)
                                                                                    gtgtGGGCGAGGCGCAGTTGCTGCTGGGGCAGAGCCTGCTGGAGGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAg                                                               <  6:47244/149‑1 (MQ=255)
                                                                                         gTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATgg                                                          >  8:170211/1‑149 (MQ=255)
                                                                                         gTCGAGGCGCACTTGCTGCTCGGGCAGATCCTGCTGGATGCGCGTCGCATCCACCAGGATGCCACCCTTGCCCGGCCTTGGTTCGGTATCGCTGCACACCTTGGCACTGCCAAAGCCCACAACATGCACCGGCCTTGACTGGAGCaacg                                                          >  2:346756/1‑146 (MQ=255)
                                                                                          tCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGCGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCa                                                                                                     <  1:157368/105‑1 (MQ=255)
                                                                                          tCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGCGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCa                                                                                                     >  2:157368/1‑105 (MQ=255)
                                                                                           cGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCacaac                                                                                    >  1:99740/1‑121 (MQ=255)
                                                                                           cGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCacaac                                                                                    <  2:99740/121‑1 (MQ=255)
                                                                                            gAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTg                                                       <  7:170211/149‑1 (MQ=255)
                                                                                                 gcAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTg                                                 >  3:202411/1‑149 (MQ=255)
                                                                                                    gTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCg                                              <  4:202411/149‑1 (MQ=255)
                                                                                                      tcatgctgTGGCAGATCCTGCTGGATGGGCGTGGCATCCAGAAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGa                                             <  4:155283/145‑1 (MQ=255)
                                                                                                           ctgGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGaa                                       >  3:339764/1‑149 (MQ=255)
                                                                                                               ggCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTg                                   <  7:255020/149‑1 (MQ=255)
                                                                                                                gCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTgg                                  <  6:285159/149‑1 (MQ=255)
                                                                                                                  agttgctgctgGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTGGGTATCGCTGGACAGGGTGGGAGTGCCTTGGCGCACAACATGGTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTg                                <  1:346756/144‑1 (MQ=255)
                                                                                                                     tCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGa                                                                <  4:240799/115‑1 (MQ=255)
                                                                                                                     tCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGa                                                                >  3:240799/1‑115 (MQ=255)
                                                                                                                      ccTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAgg                            >  6:254165/1‑149 (MQ=255)
                                                                                                                        tgctgGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATgg                                                          >  7:293338/1‑118 (MQ=255)
                                                                                                                        tgctgGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATgg                                                          <  8:293338/118‑1 (MQ=255)
                                                                                                                          ctgGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAggcggc                        >  6:139822/1‑149 (MQ=255)
                                                                                                                                 accGTGGCATCCTGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTg                                                       >  2:69558/3‑112 (MQ=255)
                                                                                                                                 accGTGGCATCCTGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTg                                                       <  1:69558/110‑1 (MQ=255)
                                                                                                                                  cgatGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGTGTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCAc                 <  1:53506/146‑1 (MQ=255)
                                                                                                                                   cGTGGCATCCTGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTa               >  7:47912/1‑149 (MQ=255)
                                                                                                                                    gTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTAc              >  5:75604/1‑149 (MQ=255)
                                                                                                                                       gCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAggcggc                        <  6:55382/136‑1 (MQ=255)
                                                                                                                                       gCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAggcggc                        >  5:55382/1‑136 (MQ=255)
                                                                                                                                       gCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTACGcc           >  2:214588/1‑149 (MQ=255)
                                                                                                                                        cATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTACGccc          >  5:76156/1‑149 (MQ=255)
                                                                                                                                          tCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTACGCCCGt        >  8:216430/1‑149 (MQ=255)
                                                                                                                                              gcagGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTg                                >  1:37292/1‑121 (MQ=255)
                                                                                                                                              gcagGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTg                                <  2:37292/121‑1 (MQ=255)
                                                                                                                                               cagGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTACGCCCGTGCGGc   <  7:216430/149‑1 (MQ=255)
                                                                                                                                                agGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTACGCCCGTGCGGcc  >  1:196104/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
TGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGG‑GTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTACGCCCGTGCGGCC  >  NC_002947/1001052‑1001344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑