Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,004,350 C→G 7.7% A125A (GCC→GCG)  PP_0866 → transmembrane sensor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,004,3500CG 7.7% 163.6 / 4.4 52A125A (GCC→GCG) PP_0866transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base C (23/25);  new base G (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01

CGGCCAGACCCGGTGCAGCAACTGCCACGACGGCGTTTCATGCGCCAGGCGTTGGCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGT  >  NC_002947/1004207‑1004497
                                                                                                                                               |                                                                                                                                                   
cGGCCAGACCCGGTGCAGCAACTGCCACGACGGCGTTTCATGCGCCAGGCGTTGGCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCaa                                                                                                                                                <  7:36788/149‑1 (MQ=255)
               cagcaACTGCCACGACGGCGTTTCATGCGCCAGGCGTTGGCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCtt                                                                                                                                                   >  2:90325/1‑131 (MQ=255)
               cagcaACTGCCACGACGGCGTTTCATGCGCCAGGCGTTGGCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCtt                                                                                                                                                   <  1:90325/131‑1 (MQ=255)
                 gcaACTGCCACGACGGCGTTTCATGCGCCAGGCGTTGGCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCGCTCATTGAAGGCCGTctgca                                                                                                                                 >  6:223621/1‑143 (MQ=255)
                 gcaACTGCCACGACGGCGTTTCATGCGCCAGGCGTTGGCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCGCTCATTGAAGGCCGTctgca                                                                                                                                 <  5:223621/147‑5 (MQ=255)
                         cACGACGGCGTTTCATGCGCCAGGCGTTGGCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCg                                                                                                                       <  3:237213/149‑1 (MQ=255)
                          acgacgGCGTTTCATGCGCCAGGCGTTGGCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCgg                                                                                                                      <  7:116343/149‑1 (MQ=255)
                               ggCGTTTCATGCGCCAGGCGTTGGCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGc                                                                                                                 <  6:216492/149‑1 (MQ=255)
                                gCGTTTCATGCGCCAGGCGTTGGCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGTGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCt                                                                                                                <  1:272484/149‑1 (MQ=255)
                                   tttCATGCGCCAGGCGTTGGCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGc                                                                                                             <  7:286162/149‑1 (MQ=255)
                                          cgcCAGGCGTTGGCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTg                                                                                                        >  5:270868/1‑147 (MQ=255)
                                          cgcCAGGCGTTGGCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGa                                                                                                      <  5:102324/149‑1 (MQ=255)
                                             cAGGCGTTGGCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCGCTCATTGAAGGCCGTctgca                                                                                                                                 >  5:151781/1‑115 (MQ=255)
                                             cAGGCGTTGGCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCGCTCATTGAAGGCCGTctgca                                                                                                                                 <  6:151781/119‑5 (MQ=255)
                                                  gTTGGCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATg                                                                                              <  8:272239/149‑1 (MQ=255)
                                                   ttGGCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATgg                                                                                             >  6:263521/1‑149 (MQ=255)
                                                     ggCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGt                                                                                                                                 <  5:272747/111‑1 (MQ=255)
                                                     ggCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGt                                                                                                                                 >  6:272747/1‑111 (MQ=255)
                                                     ggCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGc                                                                                            >  1:35409/1‑148 (MQ=255)
                                                          ccAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACg                                                                                      >  2:232852/1‑149 (MQ=255)
                                                            aGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCa                                                                                    <  2:35409/149‑1 (MQ=255)
                                                                   cgGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAg                                                                             >  1:165433/1‑149 (MQ=255)
                                                                          ggCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGt                                                                      >  4:231222/1‑149 (MQ=255)
                                                                                gggcctgggcTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAgg                                                                            >  5:314027/1‑137 (MQ=255)
                                                                                gggcctgggcTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAgg                                                                            <  6:314027/137‑1 (MQ=255)
                                                                                   cctgggcTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCg                                                             >  1:36998/1‑149 (MQ=255)
                                                                                     tgggcTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTg                                                                                >  2:316886/1‑128 (MQ=255)
                                                                                     tgggcTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTg                                                                                <  1:316886/128‑1 (MQ=255)
                                                                                            gAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGc                                                    <  1:232852/149‑1 (MQ=255)
                                                                                            gAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGc                                                    <  5:263521/149‑1 (MQ=255)
                                                                                                         caccTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGaa                                       >  7:58854/1‑149 (MQ=255)
                                                                                                             tGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCt                                   >  4:351170/1‑149 (MQ=255)
                                                                                                                 aCTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTg                                         <  2:270268/139‑1 (MQ=255)
                                                                                                                 aCTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTg                                         >  1:270268/1‑139 (MQ=255)
                                                                                                                  cTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTg                               >  6:360689/1‑148 (MQ=255)
                                                                                                                     aaCCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGt                           >  2:80727/1‑149 (MQ=255)
                                                                                                                          tCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGt                      >  7:252689/1‑149 (MQ=255)
                                                                                                                            aGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGc                                                           <  4:2952/110‑1 (MQ=255)
                                                                                                                            aGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGc                                                           >  3:2952/1‑110 (MQ=255)
                                                                                                                              ggCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAg                  >  3:55608/1‑149 (MQ=255)
                                                                                                                              ggCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAg                  <  3:231222/149‑1 (MQ=255)
                                                                                                                              ggCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAg                  >  8:50249/1‑149 (MQ=255)
                                                                                                                               gCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGc                 <  6:270868/149‑1 (MQ=255)
                                                                                                                                    cTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGc            <  4:55608/149‑1 (MQ=255)
                                                                                                                                    cTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGc            >  2:248575/1‑149 (MQ=255)
                                                                                                                                     tGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGt               >  4:279169/1‑145 (MQ=255)
                                                                                                                                     tGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGt               <  3:279169/145‑1 (MQ=255)
                                                                                                                                        aGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCAt        <  1:248575/149‑1 (MQ=255)
                                                                                                                                          aCGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGa      >  1:310440/1‑149 (MQ=255)
                                                                                                                                           cGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGa      <  5:360689/148‑1 (MQ=255)
                                                                                                                                            ggCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACa    <  3:351170/149‑1 (MQ=255)
                                                                                                                                            ggCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACa    <  8:58854/149‑1 (MQ=255)
                                                                                                                                               cTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGt  >  6:171026/1‑148 (MQ=255)
                                                                                                                                               cTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGt  >  5:243362/1‑148 (MQ=255)
                                                                                                                                               cTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGt  >  4:253597/1‑148 (MQ=255)
                                                                                                                                               cTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGt  >  8:136861/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
CGGCCAGACCCGGTGCAGCAACTGCCACGACGGCGTTTCATGCGCCAGGCGTTGGCCGCCAGTGTCGCGGTAGTGGCGCTGGGCCTGGGCTGGAGCGGCTGGCACCACCTGCAACTGAACCATCAGGGCAGCCTGCAGACGGCCTTCAATGAGCGCCGTCAGGTCGAACTGCCGGATGGCTCGCATCTGGAGCTCAATGGCAACACGCAGTTGCAGGTCGAGTTCAGCGCCGGCCAGCGGCACGTGCGCCTGAACGCTGGTGAAGTGATGTTCAGCGTCGCCCATGACAGT  >  NC_002947/1004207‑1004497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑