Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,031,920	T→A	9.3%	L5* (TTG→TAG) ‡	PP_0889 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,031,920	0	T	A	9.3%	186.7 / 6.9	64	L5* (TTG→TAG) ‡	PP_0889	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (30/28);  new base A (3/3);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATCTCGGCCACCGCACGGGCGCTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTGCCACCCTCGACTGG  >  NC_002947/1031773‑1032065                                                                                                                                                    |                                                                                                                                                  atatCTCGGCCACCGCACGGGCGCTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTg                                                                                                                                                  >  6:292546/1‑149 (MQ=255)     ctcGGCCACCGCACGGGCGCTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCcg                                                                                                                                              >  8:193066/1‑149 (MQ=255)           cACCGCACGGGCGCTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAgg                                                                                                                                        <  1:173444/149‑1 (MQ=255)           cACCGCACGGGCGCTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCAAGCCcgcgctca                                                                                                                                        <  2:260071/149‑5 (MQ=255)                    ggCGCTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAg                                                                                                                               >  3:20809/1‑149 (MQ=255)                    ggCGCTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAg                                                                                                                               <  4:26693/149‑1 (MQ=255)                    ggCGCTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAg                                                                                                                               <  2:34087/149‑1 (MQ=255)                      cgcTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCa                                                                                                                             <  6:273288/149‑1 (MQ=255)                      cgcTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCAAGCCcgcgc                                                                                                                                           <  3:7601/135‑2 (MQ=255)                      cgcTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCAAGCCcgcgc                                                                                                                                           >  4:7601/1‑134 (MQ=255)                                  aCCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAg                                                                                                                 >  1:82552/1‑149 (MQ=255)                                  aCCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAg                                                                                                                 <  2:120176/149‑1 (MQ=255)                                    cGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGc                                                                                                                                   <  7:272003/129‑1 (MQ=255)                                    cGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGc                                                                                                                                   >  8:272003/1‑129 (MQ=255)                                                  gcgcAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAgg                                                                                                 >  2:251547/1‑149 (MQ=255)                                                   cgcAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAgg                                                                                                 >  2:293517/1‑148 (MQ=255)                                                     cAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTAc                                                                                              <  7:193066/149‑1 (MQ=255)                                                         cTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGtt                                                                                          <  4:141417/149‑1 (MQ=255)                                                            cAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTgg                                                                                        >  3:229398/1‑148 (MQ=255)                                                             aGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGt                                                                                       >  2:78983/1‑148 (MQ=255)                                                              gAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCa                                                                                     >  1:266075/1‑149 (MQ=255)                                                                    gCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGc                                                                                                               <  6:19368/117‑1 (MQ=255)                                                                    gCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGc                                                                                                               >  5:19368/1‑117 (MQ=255)                                                                    gCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTg                                                                               >  6:41692/1‑149 (MQ=255)                                                                     ccGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTgc                                                                              >  2:5050/1‑149 (MQ=255)                                                                           cGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTgg                                                                         <  5:41692/148‑1 (MQ=255)                                                                            ggCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGtt                                                                       <  2:228802/149‑1 (MQ=255)                                                                               gcTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTg                                                                      >  8:70810/1‑147 (MQ=255)                                                                               gcTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTg                                                                      >  1:34132/1‑147 (MQ=255)                                                                               gcTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCa                                                                    <  1:5050/149‑1 (MQ=255)                                                                                cTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAg                                                                   <  3:160721/149‑1 (MQ=255)                                                                                        ttATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAg                                                                                                  >  6:196936/1‑110 (MQ=255)                                                                                          aTCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCTAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCa                                                          >  3:62718/1‑148 (MQ=255)                                                                                              atgatgTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGAGGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGcg                                                     >  5:114625/1‑148 (MQ=255)                                                                                               tgatgTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCt                                                     >  8:166213/1‑148 (MQ=255)                                                                                                  tgTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCTAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGaa                                                  <  4:62718/148‑1 (MQ=255)                                                                                                      ggCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACt                                              <  5:292546/148‑1 (MQ=255)                                                                                                       gCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTg                                             <  7:70810/148‑1 (MQ=255)                                                                                                       gCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTg                                             <  2:266075/148‑1 (MQ=255)                                                                                                       gCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTAGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTg                                             <  1:251547/148‑1 (MQ=255)                                                                                                        ccTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAg                                                                                                                               >  3:115946/1‑65 (MQ=255)                                                                                                        ccTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAg                                                                                                                               <  4:115946/65‑1 (MQ=255)                                                                                                        ccTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGc                                           <  2:82552/149‑1 (MQ=255)                                                                                                        ccTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGc                                           <  1:78983/149‑1 (MQ=255)                                                                                                              cgcgGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCATAGCTGTCCCATTCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGAc                                      <  1:293517/148‑1 (MQ=255)                                                                                                               gcgGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGAccc                                    >  2:345323/1‑149 (MQ=255)                                                                                                                 gggggAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCg                                   >  1:92208/1‑148 (MQ=255)                                                                                                                  ggggAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGAc                                      <  5:44712/144‑1 (MQ=255)                                                                                                                  ggggAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGAc                                      >  6:44712/1‑144 (MQ=255)                                                                                                                      aaTCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTgg                             >  2:112005/1‑149 (MQ=255)                                                                                                                        tCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCa                           <  2:92208/149‑1 (MQ=255)                                                                                                                            cTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAAc                       <  4:20809/149‑1 (MQ=255)                                                                                                                               cTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGAc                    >  7:118875/1‑149 (MQ=255)                                                                                                                               cTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGAc                    >  5:227645/1‑149 (MQ=255)                                                                                                                                  cAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGAcc                   <  2:34132/147‑1 (MQ=255)                                                                                                                                  cAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCt                 <  8:118875/149‑1 (MQ=255)                                                                                                                                   aaCTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCCGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTg                >  8:270157/1‑149 (MQ=255)                                                                                                                                       gtgAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTGCCAc            >  7:334912/1‑149 (MQ=255)                                                                                                                                       cccttcgcgGGCAAGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTGCCAc            >  4:55862/6‑149 (MQ=255)                                                                                                                                          ttcgcgGGCAAGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCaaa                                                        <  5:243488/100‑1 (MQ=255)                                                                                                                                          ttcgcgGGCAAGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCaaa                                                        >  6:243488/3‑102 (MQ=255)                                                                                                                                           gcgcGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGa                                       <  6:340997/118‑1 (MQ=255)                                                                                                                                           gcgcGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGa                                       >  5:340997/1‑118 (MQ=255)                                                                                                                                               gggCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTGCCACCCTCGACt    >  3:58729/1‑149 (MQ=255)                                                                                                                                                   ttGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTGCCACCCTCGACTgg  >  6:330247/1‑147 (MQ=255)                                                                                                                                                   ttGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTGCCACCCTCGACTgg  >  5:118412/1‑147 (MQ=255)                                                                                                                                                    |                                                                                                                                                  ATATCTCGGCCACCGCACGGGCGCTGGGCATGCACCGACGGACGTTGCAGCGCAAGCTGCAGAAGCGGCCGGTTCGGCGCTGATTCCTTATCGATGATGTTGGCCTGTTCGCGGGGGAATCCGCTCCTGCAACTGTGAGCGCGGGCTTGCCCGCGAAGGGCTGCAAAGCAGTCCCAATCAAGGCGAGCTCGATGAAGGTACTGTTGGTCATCCTTGCGCTGGTTGCAGGTAGCGCCAAAGCTGAAGACTGGCCTGACCCGCAGTGGCAGAACGACCCTGCCACCCTCGACTGG  >  NC_002947/1031773‑1032065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑