Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,036,818 C→T 9.2% R175C (CGC→TGC)  PP_0895 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,036,8180CT 9.2% 119.4 / 2.4 44R175C (CGC→TGC) PP_0895hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (20/19);  new base T (2/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01

GCCGAGTTCGATTACTGCGCGTTCTGCGCGGTCGAGCGACCCGGTGCGCACCCGCGACGGCCAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTACGCCATCGAGCTGCACGAAACCTGGGACGCCTACGCCGACCACCTGCAAGGCCTGTG  >  NC_002947/1036674‑1036965
                                                                                                                                                 |                                                                                                                                                   
gCCGAGTTCGATTACTGCGCGTTCTGCGCGGTCGAGCGACCCGGTGCGCACCCGCGACGGCCAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGa                                                                                                                                                 >  4:199345/1‑149 (MQ=255)
gCCGAGTTCGATTACTGCGCGTTCTGCGCGGTCGAGCGACCCGGTGCGCACCCGCGACGGCCAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGa                                                                                                                                                 >  2:2076/1‑149 (MQ=255)
  cGAGTTCGATTACTGCGCGTTCTGCGCGGTCGAGCGACCCGGTGCGCACCCGCGACGGCCAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGAcc                                                                                                                                               >  1:314178/1‑149 (MQ=255)
     gTTCGATTACTGCGCGTTCTGCGCGGTCGAGCGACGCGGTGCGCACCCGCGACGGCCAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTgg                                                                                                                                            <  7:291978/149‑1 (MQ=255)
     gTTCGATTACTGCGCGTTCTGCGCGGTCGAGCGACCCGGTGCGCACCCGCGACGGCCAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTgg                                                                                                                                            <  6:361524/149‑1 (MQ=255)
                 cgcgTTCTGCGCGGTCGAGCGACCCGGTGCGCACCCGCGACGGCCAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGaaa                                                                                                                                <  3:319688/149‑1 (MQ=255)
                 cgcgTTCTGCGCGGTCGAGCGACCCGGTGCGCACCCGCGACGGCCAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGa                                                                                                                                  <  1:49884/147‑1 (MQ=255)
                          cgcgGTCGAGCGACCCGGTGCGCACCCGCGACGGCCAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGa                                                                                                                         <  2:3108/147‑1 (MQ=255)
                                   gCGACCCGGTGCGCACCCGCGACGGCCAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGt                                                                                                              <  1:320370/149‑1 (MQ=255)
                                       cccGGTGCGCACCCGCGACGGCCAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCg                                                                                                            >  8:231288/1‑147 (MQ=255)
                                       cccGGTGCGCACCCGCGACGGCCAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCGTCCT‑GCACGACCCATCTCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCg                                                                                                            <  7:231288/147‑1 (MQ=255)
                                          ggTGCGCACCCGCGACGGCCAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAg                                                                                                                     <  1:62126/135‑1 (MQ=255)
                                          ggTGCGCACCCGCGACGGCCAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAg                                                                                                                     >  2:62126/1‑135 (MQ=255)
                                                 aCCCGCGACGGCCAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAg                                                                                                >  6:74231/1‑149 (MQ=255)
                                                  cccGCGACGGCCAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAgg                                                                                               <  5:74231/149‑1 (MQ=255)
                                                            ccAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAg                                                                                                                     >  5:216005/1‑117 (MQ=255)
                                                            ccAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAg                                                                                                                     <  6:216005/117‑1 (MQ=255)
                                                            ccAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGt                                                                                     <  5:202136/149‑1 (MQ=255)
                                                              aGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCTGGCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTa                                                                                    >  1:84659/1‑149 (MQ=255)
                                                                       cAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGcc                                                                           <  2:84659/148‑1 (MQ=255)
                                                                       cAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCt                                                                          <  3:199345/149‑1 (MQ=255)
                                                                        aaaCCCCTGCACGGCTTCTGGCGCACCCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTg                                                                         >  8:296902/1‑149 (MQ=255)
                                                                        aaaCCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGTGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGc                                                                            >  1:286806/1‑146 (MQ=255)
                                                                        aaaCCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGTGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGc                                                                            <  2:286806/146‑1 (MQ=255)
                                                                        aaaCCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTAt                                                                                   >  3:259195/1‑139 (MQ=255)
                                                                        aaaCCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTAt                                                                                   <  4:259195/139‑1 (MQ=255)
                                                                         aaCCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTgg                                                                        <  2:314178/149‑1 (MQ=255)
                                                                                       ttCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTAc                                                          >  4:342407/1‑149 (MQ=255)
                                                                                          tGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTACGc                                                        >  6:226246/1‑148 (MQ=255)
                                                                                              gcAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGTGCGACCAGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTACGCCATc                                                    >  3:147401/1‑148 (MQ=255)
                                                                                                      ggCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTACGCCATCGAGCTGCAc                                           <  3:342407/149‑1 (MQ=255)
                                                                                                       gCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTACGCCATCGAGCTGCAc                                           >  6:177570/1‑148 (MQ=255)
                                                                                                          tCCT‑GCACGAACCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCAGGTATGATCCGCCTGGCAGCCTGCCAGTACGCCATCGAGCTGCACGaaa                                       >  5:125192/1‑149 (MQ=255)
                                                                                                             t‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTACGCCATCGAGCTGCACGAAACCt                                    >  2:311013/1‑149 (MQ=255)
                                                                                                                cACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTACGCCATCGAGCTGCAc                                           <  8:39971/140‑1 (MQ=255)
                                                                                                                cACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTACGCCATCGAGCTGCAc                                           >  7:39971/1‑140 (MQ=255)
                                                                                                                   gACCCATCGCTGCCTACCACCTACGCCTGGAGCGACCTGGACGAGCAGGAAAGCTCCCACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTACGCCATCAAGCTGCACGAAACCTGGGAc                               >  6:182164/1‑149 (MQ=255)
                                                                                                                        aTCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGATCCGACAAGCTGATGTCGTTCTGGCTCACGGCAGTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTACGCCATCGAGCTGACCGAAACCTGGGACTCCTa                          >  2:332094/1‑149 (MQ=255)
                                                                                                                           gCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTACGCCATCAAGCTGCACGAAACCTGGGACGCCTACg                        <  5:182164/148‑1 (MQ=255)
                                                                                                                               cGTACCACCTACGCCTGGTGCGACCAGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTACGCCATCGAGCTGCACGAAACCTGGGACGCCTACGCCGac                   <  4:147401/149‑1 (MQ=255)
                                                                                                                                        tACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTACGCCATCGAGCTGCACGAAACCTGGGACGCCTACGCCGACCACCTGc            >  6:180121/1‑147 (MQ=255)
                                                                                                                                           gCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTACGCCATCGAGCTGCACGAAACCTGGGACGCCTACGCCGACCACCTGCAAGGc       >  8:114269/1‑149 (MQ=255)
                                                                                                                                             cTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTACGCCATCGAGCTGCACGAAACCTGGGACGCCTACGCCGACCACCTGCAAGGCCt     <  5:177570/149‑1 (MQ=255)
                                                                                                                                             cTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTACGCCATCGAGCTGCACGAAACCTGGGACGCCTACGCCGACCACCTGCAAGGCCt     >  6:199660/1‑149 (MQ=255)
                                                                                                                                                gcgcGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTACGCCATCGAGCTGCACGAAACCTGGGACGCCTACGCCGACCACCTGCAAGGCCtgtg  <  5:199660/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCCGAGTTCGATTACTGCGCGTTCTGCGCGGTCGAGCGACCCGGTGCGCACCCGCGACGGCCAGCCGAGTACAAACCCCTGCACGGCTTCTGGCGCAACCGCGGCTTCCT‑GCACGACCCATCGCTGCGTACCACCTACGCCTGGCGCGACCTGGACGAGCAGGAAAGCTCCGACAAGCTGATGTCGTTCTGGCTCAAGGCACTGCCGGTATGATCCGCCTGGCAGCCTGCCAGTACGCCATCGAGCTGCACGAAACCTGGGACGCCTACGCCGACCACCTGCAAGGCCTGTG  >  NC_002947/1036674‑1036965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑