Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,037,951 T→C 5.9% N482D (AAC→GAC)  PP_0897 ← class 1 fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,037,9510TC 5.9% 199.3 / 2.0 68N482D (AAC→GAC) PP_0897class 1 fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base T (33/31);  new base C (2/2);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

CCTTCTGGCTTGCGCTGTACGTGTGGGAGCGGGCTTGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACTTGCGGATGGCCTGGGCGACCAGGTAGGCAGCGCCGCCAACGGCCAT  >  NC_002947/1037803‑1038089
                                                                                                                                                    |                                                                                                                                          
ccTTCTGGCTTGCGCTGTACGTGTGGGAGCGGGCTTGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGtt                                                                                                                                            <  5:242148/149‑1 (MQ=255)
  ttCTGGCTTGCGCTGTACGTGTGGGAGCGGGCTTGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGc                                                                                                                                          >  4:148120/1‑149 (MQ=255)
    cTGGCTTGCGCTGTACGTGTGGGAGCGGGCTTGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCt                                                                                                                                         <  4:108227/148‑1 (MQ=255)
     tGGCTTGCGCTGTACGTGTGGGAGCGGGCTTGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGt                                                                                                                                       >  5:117603/1‑149 (MQ=255)
            cgcTGTACGTGTGGGAGCGGGCTTGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACgg                                                                                                                                 <  7:100822/148‑1 (MQ=255)
                        gggAGCGGGCTTGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTAGCGATTCACCGTCGCCGTGGACAGCAACGGt                                                                                                                          <  1:30054/143‑1 (MQ=255)
                        gggAGCGGGCTTGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTCGCCGTGGACAGCAACGGt                                                                                                                          >  2:30054/1‑143 (MQ=255)
                        cttcgCGGGCAAGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCgg                                                                                                                    >  1:50578/5‑149 (MQ=255)
                         ggAGCGGGCTTGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGc                                                                                                                                <  7:211454/136‑1 (MQ=255)
                         ggAGCGGGCTTGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGc                                                                                                                                >  8:211454/1‑136 (MQ=255)
                         ggAGCGGGCTTGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCgg                                                                                                                    >  6:331545/1‑148 (MQ=255)
                           aGCGGGCTTGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCAt                                                                                                                 <  6:117603/149‑1 (MQ=255)
                            gCGGGCTTGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACg                                                                                                                            <  3:34942/137‑1 (MQ=255)
                            gCGGGCTTGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACg                                                                                                                            >  4:34942/1‑137 (MQ=255)
                            gCGGGCTTGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATg                                                                                                                >  4:293734/1‑149 (MQ=255)
                               ggCTTGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTcc                                                                                                             >  4:61383/1‑149 (MQ=255)
                                  ttGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACgg                                                                                                                                 >  3:252224/1‑126 (MQ=255)
                                  ttGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACgg                                                                                                                                 <  4:252224/126‑1 (MQ=255)
                                    gCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCTGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGAc                                                                                                        >  7:197753/1‑149 (MQ=255)
                                      ccGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACtt                                                                                                      >  7:196897/1‑149 (MQ=255)
                                      ccGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACtt                                                                                                      >  7:202204/1‑149 (MQ=255)
                                       cgcgAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTc                                                                                                     <  3:148120/149‑1 (MQ=255)
                                         cgAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGa                                                                                                   <  3:293734/149‑1 (MQ=255)
                                             gcagcCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCTGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTc                                                                                               <  8:197753/149‑1 (MQ=255)
                                             gcagcCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTGACCGTTGCTGTCCACGGCGTCGGTCACCGGCATGTCCTTGACTTCGAACTc                                                                                               <  5:331545/149‑1 (MQ=255)
                                                         ggtccATCAGGTTACTGCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATGCACCGGTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTc                                                                                   <  8:202204/145‑1 (MQ=255)
                                                         gTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTc                                                                                   <  8:196897/149‑1 (MQ=255)
                                                                 aGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGcc                                                                             >  2:86971/1‑147 (MQ=255)
                                                                 aGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGcc                                                                             >  4:297778/1‑147 (MQ=255)
                                                                  ggTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAg                                                                          >  5:294903/1‑149 (MQ=255)
                                                                  ggTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAg                                                                          >  7:104271/1‑149 (MQ=255)
                                                                   ggtACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCACTGCCACAGGGCAGGGCCAGTGATGTGAACCGATACACCGTTGCTGTACACGGCCACGGTCACCGGCATTTCCTTGACTTCCAACTCGTAGAACGCTTCCATGCCCAg                                                                          >  3:234050/3‑148 (MQ=255)
                                                                        ttcacttcGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCAt                                                                                >  3:266779/1‑137 (MQ=255)
                                                                        ttcacttcGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCAt                                                                                <  4:266779/137‑1 (MQ=255)
                                                                              tcGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGc                                                              >  2:138084/1‑149 (MQ=255)
                                                                               cGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGc                                                              <  1:138084/148‑1 (MQ=255)
                                                                                  cGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGt                                                          <  1:138440/149‑1 (MQ=255)
                                                                                     ccAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGccc                                                                            >  5:218037/1‑128 (MQ=255)
                                                                                     ccAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGccc                                                                            <  6:218037/128‑1 (MQ=255)
                                                                                     ccAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTAcc                                                       >  2:181638/1‑149 (MQ=255)
                                                                                      cAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTCGCCGTGGACAGCAACGGt                                                                                                                          >  8:162569/1‑81 (MQ=37)
                                                                                      cAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTCGCCGTGGACAGCAACGGt                                                                                                                          <  7:162569/81‑1 (MQ=37)
                                                                                        ggCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTc                                                    <  2:50578/149‑1 (MQ=255)
                                                                                        ggCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTc                                                    <  3:61383/149‑1 (MQ=255)
                                                                                          cggtCGGCAATCTTGCTCTGCCCCAGGGCAGGGCCAGTGATGTGTCCCGATTCACCCTTGCTGTCCACCGCGACGGTCACCGGCATCTCCTTGCCATCGAACTCCTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTGCGa                                                  <  8:104271/146‑1 (MQ=255)
                                                                                               ggCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACTTGc                                             <  8:242654/149‑1 (MQ=255)
                                                                                               ggCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACTTGc                                             >  1:286961/1‑149 (MQ=255)
                                                                                               ggCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACTTGc                                             >  7:242654/1‑149 (MQ=255)
                                                                                                   aTCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACTTGCGGAt                                         <  6:294903/149‑1 (MQ=255)
                                                                                                   aTCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACTTGCGGAt                                         <  2:286961/149‑1 (MQ=255)
                                                                                                    tCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGc                                                              >  1:318420/1‑127 (MQ=255)
                                                                                                    tCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGc                                                              <  2:318420/127‑1 (MQ=255)
                                                                                                     cTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACt                                                >  8:303736/1‑140 (MQ=255)
                                                                                                     cTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACt                                                <  7:303736/140‑1 (MQ=255)
                                                                                                        gCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACTTGCGGATGGc                                      <  3:297778/147‑1 (MQ=255)
                                                                                                            tGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACTTGCGGATGGCCTGGGc                                >  4:137528/1‑149 (MQ=255)
                                                                                                              ccACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACTTGCGGATGGCCTGGGCGa                              <  5:293905/149‑1 (MQ=255)
                                                                                                              ccACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACTTGCGGATGGCCTGGGCGa                              >  3:112442/1‑149 (MQ=255)
                                                                                                               cacaGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACTTGCGGATGGCCTGGGCGAc                             <  3:137528/149‑1 (MQ=255)
                                                                                                                  agggcagggcCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACTTGCGGATGGCCTGGGCGACCAg                          >  3:317627/1‑149 (MQ=255)
                                                                                                                      cagggcCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACTTGCGGATGGCCTGGGCGACCAGGTAg                      <  5:295103/149‑1 (MQ=255)
                                                                                                                            cAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGa                                                  >  6:239762/1‑115 (MQ=255)
                                                                                                                            cAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGa                                                  <  5:239762/115‑1 (MQ=255)
                                                                                                                                     gtACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGATCTCGTAGATCGCTTCCATGACCAGCTCGGCGAAGCCCAGTACCTTCGACTTGCGGATGGCCTGGGCGACCAGGTAGGCAGCGCCGACAACg       >  5:42090/1‑149 (MQ=255)
                                                                                                                                      tACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACTTGCGGATGGCCTGGGCGACCAGGTAGGCAGCGCCGCCAACgg      >  2:202661/1‑149 (MQ=255)
                                                                                                                                      tACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACTTGCGGATGGCCTGGGCGACCAGGTAGGCAGCGCCGCCAACg       <  3:158879/148‑1 (MQ=255)
                                                                                                                                            ttCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACTTGCGGATGGCCTGGGCGACCAGGTAGGCAGCGCCGCCAACGGCCAt  >  6:313875/1‑147 (MQ=255)
                                                                                                                                               aCCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCTGCGAAGGCCAGTACCTTCGACTTGCGGATGGCCTGGGCGACCAg                          <  2:230277/120‑1 (MQ=255)
                                                                                                                                               aCCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACTTGCGGATGGCCTGGGCGACCAg                          >  1:230277/1‑120 (MQ=255)
                                                                                                                                                    |                                                                                                                                          
CCTTCTGGCTTGCGCTGTACGTGTGGGAGCGGGCTTGCCCGCGAAGCAGCCGCCGCAGTTACATCAGGTTACTTCACTTCGACGGCCAGGCTTTCGGCAATCTTGCTCTGCCACAGGGCAGGGCCAGTGATGTGTACCGATTCACCGTTGCTGTCCACGGCGACGGTCACCGGCATGTCCTTGACTTCGAACTCGTAGATCGCTTCCATGCCCAGCTCGGCGAAGGCCAGTACCTTCGACTTGCGGATGGCCTGGGCGACCAGGTAGGCAGCGCCGCCAACGGCCAT  >  NC_002947/1037803‑1038089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑