Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,045,262 G→A 8.1% intergenic (+35/+71) PP_0905 → / ← PP_0906 hypothetical protein/RND family multidrug transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,045,2620GA 8.1% 139.1 / 2.2 49intergenic (+35/+71)PP_0905/PP_0906hypothetical protein/RND family multidrug transporter
Reads supporting (aligned to +/- strand):  ref base G (22/23);  new base A (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCGCGTTGCCGCGGGATGCAAAACCGCAGCAGCAGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTAGATGCACGGCAACACAAACAAGG  >  NC_002947/1045116‑1045406
                                                                                                                                                  |                                                                                                                                                
gTGCGCGTTGCCGCGGGATGCAAAACCGCAGCAGCAGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGAt                                                                                                                                                >  8:114933/1‑149 (MQ=255)
     cgTTGCCGCGGGATGCAAAACCGCAGCAGCAGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCgg                                                                                                                                           <  6:78827/149‑1 (MQ=255)
            gcgGGATGCAAAACCGCAGCAGCAGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCttt                                                                                                                                    >  6:360701/1‑149 (MQ=255)
             cgGGATGCAAAACCGCAGCAGCAGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTg                                                                                                                                   <  7:114933/149‑1 (MQ=255)
                    cAAAACCGCAGCAGCAGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGtttttt                                                                                                                            <  8:312924/149‑1 (MQ=255)
                     aaaaCCGCAGCAGCAGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTg                                                                                                                           >  6:178991/1‑149 (MQ=255)
                       aaCCGCAGCAGCAGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCg                                                                                                                         >  6:185685/1‑149 (MQ=255)
                           gcagcagcaGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCACGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTg                                                                                                                     >  5:268908/1‑149 (MQ=255)
                            cagcagcagGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGc                                                                                                                    <  6:339987/149‑1 (MQ=255)
                               cagcagGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCtt                                                                                                                 >  4:156404/1‑149 (MQ=255)
                                   agGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCg                                                                                                             <  3:156404/149‑1 (MQ=255)
                                   agGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCg                                                                                                             >  7:314542/1‑149 (MQ=255)
                                     gCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGc                                                                                                           >  5:260901/1‑149 (MQ=255)
                                     gCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCCCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGc                                                                                                           <  6:268908/149‑1 (MQ=255)
                                         ggAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCctct                                                                                                       >  8:227020/1‑149 (MQ=255)
                                               agcCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCgg                                                                                                 <  7:24996/149‑1 (MQ=255)
                                               agcCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCgg                                                                                                 <  5:178991/149‑1 (MQ=255)
                                                   aGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAg                                                                                             >  4:308885/1‑149 (MQ=255)
                                                          gACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAgcccgccc                                                                                      <  8:314542/149‑1 (MQ=255)
                                                            ccTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCCCGCAATCGCGAGGCCtct                                                                                                                                    >  5:305836/1‑99 (MQ=38)
                                                            ccTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCCCGCAATCGCGAGGCCtct                                                                                                                                    <  6:305836/101‑3 (MQ=38)
                                                             cTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGcccc                                                                                     >  8:169167/1‑147 (MQ=255)
                                                                ttGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAg                                                                                  <  5:185685/147‑1 (MQ=255)
                                                                ttGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAg                                                                                  <  2:133313/147‑1 (MQ=255)
                                                                  gCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTc                                                                               <  7:227020/148‑1 (MQ=255)
                                                                        ccTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCa                                                                         <  6:260901/148‑1 (MQ=255)
                                                                         cTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAg                                                                       <  5:360701/149‑1 (MQ=255)
                                                                            tttGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGc                                                                      <  3:308885/147‑1 (MQ=255)
                                                                                    gCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGt                                                            >  3:30886/1‑149 (MQ=255)
                                                                                         aaGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCtt                                                       >  7:100401/1‑149 (MQ=255)
                                                                                            ggTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGgc                                                    <  7:169167/149‑1 (MQ=255)
                                                                                             gTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGgcg                                                   >  4:268823/1‑149 (MQ=255)
                                                                                                   gTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGcc                                             <  4:146062/149‑1 (MQ=255)
                                                                                                    tCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCt                                            >  5:298085/1‑149 (MQ=255)
                                                                                                     cacaGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCtt                                           <  3:268823/149‑1 (MQ=255)
                                                                                                      acaGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTtg                                          >  5:203911/1‑149 (MQ=255)
                                                                                                        aGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTtgtg                                        >  6:59119/1‑149 (MQ=255)
                                                                                                                      cGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGgtg                           <  6:298085/148‑1 (MQ=255)
                                                                                                                      cGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGgtg                           <  8:100401/148‑1 (MQ=255)
                                                                                                                       gTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGgtg                           >  8:39484/1‑147 (MQ=255)
                                                                                                                       gTTAGCTGGAAAAGCCAGAGGCCTCGCGAATGCGGGGCCGTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTGCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTa                         <  3:223375/149‑1 (MQ=255)
                                                                                                                        ttAGCCGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGgtg                           >  2:8467/1‑146 (MQ=255)
                                                                                                                           gCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTAGATg                     >  5:346411/1‑149 (MQ=255)
                                                                                                                               gAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTAGATGCAc                  <  5:59119/148‑1 (MQ=255)
                                                                                                                                  aaGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTAGATGCACGGc               >  2:161156/1‑148 (MQ=255)
                                                                                                                                      caaagGCCCCGCAATCGCGAGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTAGATGCACg                 <  4:66406/139‑1 (MQ=255)
                                                                                                                                      caaagGCCCCGCAATCGCGAGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTAGATGCACg                 >  3:66406/4‑142 (MQ=255)
                                                                                                                                             cTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTAGATGCACGGCAACACAAACAAg   <  1:161156/149‑1 (MQ=255)
                                                                                                                                              tCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTAGATGCACGGCAACACAAACAAgg  >  1:120524/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GTGCGCGTTGCCGCGGGATGCAAAACCGCAGCAGCAGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTAGATGCACGGCAACACAAACAAGG  >  NC_002947/1045116‑1045406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑