Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,045,265 T→C 8.3% intergenic (+38/+68) PP_0905 → / ← PP_0906 hypothetical protein/RND family multidrug transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,045,2650TC 8.3% 130.6 / 3.3 48intergenic (+38/+68)PP_0905/PP_0906hypothetical protein/RND family multidrug transporter
Reads supporting (aligned to +/- strand):  ref base T (21/23);  new base C (2/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

CGTTGCCGCGGGATGCAAAACCGCAGCAGCAGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTAGATGCACGGCAACACAAACAAGG  >  NC_002947/1045121‑1045406
                                                                                                                                                |                                                                                                                                             
cgTTGCCGCGGGATGCAAAACCGCAGCAGCAGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCgg                                                                                                                                           <  6:78827/149‑1 (MQ=255)
       gcgGGATGCAAAACCGCAGCAGCAGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCttt                                                                                                                                    >  6:360701/1‑149 (MQ=255)
        cgGGATGCAAAACCGCAGCAGCAGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTg                                                                                                                                   <  7:114933/149‑1 (MQ=255)
               cAAAACCGCAGCAGCAGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGtttttt                                                                                                                            <  8:312924/149‑1 (MQ=255)
                aaaaCCGCAGCAGCAGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTg                                                                                                                           >  6:178991/1‑149 (MQ=255)
                  aaCCGCAGCAGCAGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCg                                                                                                                         >  6:185685/1‑149 (MQ=255)
                      gcagcagcaGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCACGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTg                                                                                                                     >  5:268908/1‑149 (MQ=255)
                       cagcagcagGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGc                                                                                                                    <  6:339987/149‑1 (MQ=255)
                          cagcagGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCtt                                                                                                                 >  4:156404/1‑149 (MQ=255)
                              agGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCg                                                                                                             <  3:156404/149‑1 (MQ=255)
                              agGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCg                                                                                                             >  7:314542/1‑149 (MQ=255)
                                gCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGc                                                                                                           >  5:260901/1‑149 (MQ=255)
                                gCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCCCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGc                                                                                                           <  6:268908/149‑1 (MQ=255)
                                    ggAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCctct                                                                                                       >  8:227020/1‑149 (MQ=255)
                                          agcCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCgg                                                                                                 <  7:24996/149‑1 (MQ=255)
                                          agcCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCgg                                                                                                 <  5:178991/149‑1 (MQ=255)
                                              aGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAg                                                                                             >  4:308885/1‑149 (MQ=255)
                                                     gACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAgcccgccc                                                                                      <  8:314542/149‑1 (MQ=255)
                                                       ccTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCCCGCAATCGCGAGGCCtct                                                                                                                                    >  5:305836/1‑99 (MQ=38)
                                                       ccTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCCCGCAATCGCGAGGCCtct                                                                                                                                    <  6:305836/101‑3 (MQ=38)
                                                        cTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGcccc                                                                                     >  8:169167/1‑147 (MQ=255)
                                                           ttGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAg                                                                                  <  5:185685/147‑1 (MQ=255)
                                                           ttGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAg                                                                                  <  2:133313/147‑1 (MQ=255)
                                                             gCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTc                                                                               <  7:227020/148‑1 (MQ=255)
                                                                   ccTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCa                                                                         <  6:260901/148‑1 (MQ=255)
                                                                    cTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAg                                                                       <  5:360701/149‑1 (MQ=255)
                                                                       tttGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGc                                                                      <  3:308885/147‑1 (MQ=255)
                                                                               gCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGt                                                            >  3:30886/1‑149 (MQ=255)
                                                                                    aaGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCtt                                                       >  7:100401/1‑149 (MQ=255)
                                                                                       ggTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGgc                                                    <  7:169167/149‑1 (MQ=255)
                                                                                        gTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGgcg                                                   >  4:268823/1‑149 (MQ=255)
                                                                                              gTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGcc                                             <  4:146062/149‑1 (MQ=255)
                                                                                               tCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCt                                            >  5:298085/1‑149 (MQ=255)
                                                                                                cacaGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCtt                                           <  3:268823/149‑1 (MQ=255)
                                                                                                 acaGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTtg                                          >  5:203911/1‑149 (MQ=255)
                                                                                                   aGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTtgtg                                        >  6:59119/1‑149 (MQ=255)
                                                                                                                 cGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGgtg                           <  8:100401/148‑1 (MQ=255)
                                                                                                                 cGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGgtg                           <  6:298085/148‑1 (MQ=255)
                                                                                                                  gTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGgtg                           >  8:39484/1‑147 (MQ=255)
                                                                                                                  gTTAGCTGGAAAAGCCAGAGGCCTCGCGAATGCGGGGCCGTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTGCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTa                         <  3:223375/149‑1 (MQ=255)
                                                                                                                   ttAGCCGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGgtg                           >  2:8467/1‑146 (MQ=255)
                                                                                                                      gCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTAGATg                     >  5:346411/1‑149 (MQ=255)
                                                                                                                          gAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTAGATGCAc                  <  5:59119/148‑1 (MQ=255)
                                                                                                                             aaGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTAGATGCACGGc               >  2:161156/1‑148 (MQ=255)
                                                                                                                                 caaagGCCCCGCAATCGCGAGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTAGATGCACg                 <  4:66406/139‑1 (MQ=255)
                                                                                                                                 caaagGCCCCGCAATCGCGAGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTAGATGCACg                 >  3:66406/4‑142 (MQ=255)
                                                                                                                                        cTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTAGATGCACGGCAACACAAACAAg   <  1:161156/149‑1 (MQ=255)
                                                                                                                                         tCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTAGATGCACGGCAACACAAACAAgg  >  1:120524/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                             
CGTTGCCGCGGGATGCAAAACCGCAGCAGCAGGCCCGGAGCGAGCCAGACCAGGACCTTTTGCTGTTCCTGTTTGGCCTGCGTCAAGGGTTGCGGTCACAGGGATGAACCCTGCGTTAGCTGGAAAAGCCAGAGGCCTCGCGATTGCGGGGCCTTTGGTTTTTTGCGTCTGCCTTGCCGGCCTCTTCGCGGGCAGCCCGCCCCAAGGTCGGTTCAAGCGATTGCAGGTGCCTTGGCGCTCGCCTTGTGCGCCAGCAGGGTGTAGATGCACGGCAACACAAACAAGG  >  NC_002947/1045121‑1045406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑