Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 61,255 G→A 9.7% R142H (CGT→CAT) ‡ PP_0052 → beta‑lactamase domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294761,2550GA 9.7% 107.8 / 2.9 41R142H (CGT→CAT) ‡PP_0052beta‑lactamase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (20/17);  new base A (2/2);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

CAATTGGCGCGCACATTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCGACTTCCCCGGGG  >  NC_002947/61114‑61389
                                                                                                                                             |                                                                                                                                       
cAATTGGCGCGCACATTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑Tg                                                                                                                                 >  3:321431/1‑149 (MQ=255)
 aaTTGGCGCGCACATTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGc                                                                                                                                <  1:72101/149‑1 (MQ=255)
   ttGGCGCGCACATTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGca                                                                                                                               >  4:171135/1‑148 (MQ=255)
      gcgcgcACATTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGAAGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTCCGT‑TGCACCCg                                                                                                                           >  8:114603/1‑149 (MQ=255)
            acaTTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTggg                                                                                                                     <  5:48811/149‑1 (MQ=255)
              aTTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGca                                                                                                                   >  6:96709/1‑149 (MQ=255)
               ggcatcggccccggggaagtcgcagcgggcggtgccatagtcgggcatgaacaaggtgtctccaacgaacaccgcgatctcgccggcatcttcgatcatgaaactcatgcacgcgggtgtgtGCCCGTGCGT‑TGCACACGCCTGGGcac                                                                                                                  >  7:137451‑M1/123‑149 (MQ=255)
                          cAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGt                                                                                                        <  5:88367/148‑1 (MQ=255)
                          cAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTg                                                                                                       >  5:46313/1‑149 (MQ=255)
                          cAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTg                                                                                                       >  5:51510/1‑149 (MQ=255)
                              aaGTGGTTGGCGCCTTGTTCAACGCAGAGCACGGCTTCGCTCTTGATGGCAGCCAGTTCGATGTGCTTCTCGAAGACGAGGAAGGTTTCATGATTTGGAAACTTCAGTCCCGTGCTT‑TGCAGTCGCCTTGGCACACACGCGGGTGCAt                                                                                                    <  4:191496/148‑1 (MQ=255)
                                          ccTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATc                                                                                       >  6:71645/1‑149 (MQ=255)
                                              gTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGaa                                                                                    <  6:46313/148‑1 (MQ=255)
                                              gTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGaa                                                                                    <  6:51510/148‑1 (MQ=255)
                                              gTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGaa                                                                                    <  5:96709/148‑1 (MQ=255)
                                               ttCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATc                                                                                       <  4:71174/144‑1 (MQ=255)
                                               ttCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGagat                                                                                  >  3:71174/1‑146 (MQ=255)
                                                 cAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGAt                                                                                 <  3:171135/148‑1 (MQ=255)
                                                               gCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATcgcg                                                                  <  2:310839/149‑1 (MQ=255)
                                                                   cGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGgt                                                                <  4:275658/147‑1 (MQ=255)
                                                                      ccGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCg                                                           >  5:226681/1‑149 (MQ=255)
                                                                        gTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATc                                                                     >  1:41507/1‑137 (MQ=255)
                                                                        gTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATc                                                                     <  2:41507/137‑1 (MQ=255)
                                                                         tGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACTCACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTg                                                        <  4:321431/149‑1 (MQ=255)
                                                                           aTGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGga                                                      <  2:131860/149‑1 (MQ=255)
                                                                            tGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCC‑GGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATAGCGGTGTTCGTTGgaga                                                    >  3:35350/1‑149 (MQ=255)
                                                                                   cAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCtt                                              >  8:14773/1‑149 (MQ=255)
                                                                                     gTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGt‑t                                                                                                                                  <  8:167865/63‑1 (MQ=255)
                                                                                     gTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGt‑t                                                                                                                                  >  7:167865/1‑63 (MQ=255)
                                                                                        cGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTc                                          <  6:160457/148‑1 (MQ=255)
                                                                                                     gAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTAt                              <  5:71645/147‑1 (MQ=255)
                                                                                                     gAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATgg                            >  7:311041/1‑149 (MQ=255)
                                                                                                              gAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCAccgcccg                   >  4:198833/1‑149 (MQ=255)
                                                                                                                   tttCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCg              >  4:32935/1‑149 (MQ=255)
                                                                                                                              gCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCGACTTCCCCggg   >  3:27011/1‑149 (MQ=255)
                                                                                                                              gCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCGACTTCCCCggg   >  3:96665/1‑149 (MQ=255)
                                                                                                                               gcgggtgtgtGCCCAGGCGTGTGCA‑ACGCACGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATcgcg                                                                  <  5:185936‑M1/75‑1 (MQ=255)
                                                                                                                               gcgggtgtgtGCCCAGGCGTGTGCA‑ACGCACGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATcgcg                                                                  >  6:185936‑M1/11‑85 (MQ=255)
                                                                                                                               gcgggtgtgtGCCCAGGCGTGTGCA‑ACGCACGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATcgc                                                                   <  7:210194‑M1/74‑1 (MQ=255)
                                                                                                                               gcgggtgtgtGCCCAGGCGTGTGCA‑ACGCACGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATcgc                                                                   >  8:210194‑M1/11‑84 (MQ=255)
                                                                                                                               cAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCGACTTCCCCgggg  >  1:197529/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                       
CAATTGGCGCGCACATTACCCAGGTGCAGAAAGTGTTTGGCGCCTTGTTCAACGCAGAGCCCGGCTTCGCCCGTGATGGCAGCCAGTTCGATGTGCTGCTCGAAGACGAGGAAGGTTTCCGGATTGGCAACCTGCAGGCCCGTGCGT‑TGCACACGCCTGGGCACACACCCGCGTGCATGAGTTTCATGATCGAAGATGCCGGCGAGATCGCGGTGTTCGTTGGAGACACCTTGTTCATGCCCGACTATGGCACCGCCCGCTGCGACTTCCCCGGGG  >  NC_002947/61114‑61389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑