Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,090,837 T→C 7.0% D97D (GAT→GAC) ‡ PP_0945 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,090,8370TC 7.0% 162.4 / 2.6 57D97D (GAT→GAC) ‡PP_0945hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (23/30);  new base C (2/2);  total (25/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01

GGCTGCATTCGTCATCGCCCACCACAACCTGGCTGACCTGCACCTGCGCCTTGACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACC  >  NC_002947/1090690‑1090980
                                                                                                                                                   |                                                                                                                                               
ggCTGCATTCGTCATCGCCCACCACAACCTGGCTGACCTGCACCTGCGCCTTGACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATg                                                                                                                                                >  5:170384/1‑149 (MQ=255)
       ttCGTCATCGCCCACCACAACCTGGCTGACCTGCACCTGCGCCTTGACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCtt                                                                                                                                         >  8:246998/1‑149 (MQ=255)
          gTCATCGCCCACCACAACCTGGCTGACCTGCACCTGCGCCTTGACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGAGTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTgc                                                                                                                                       <  3:6474/148‑1 (MQ=255)
           tCATCGCCCACCACAACCTGGCTGACCTGCACCTGCGCCTTGACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCATCACGCAGcccctgag                                                                                                                                     <  6:9857/149‑7 (MQ=255)
             aTCGCCCACCACAACCTGGCTGCCCTGCACCTGCGCCTTGACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCca                                                                                                                                   >  7:74905/1‑149 (MQ=255)
             aTCGCCCACCACAACCTGGCTGACCTGCACCTGCGCCTTGACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCca                                                                                                                                   >  3:183369/1‑149 (MQ=255)
             aTCGCCCACCACAACCTGGCTGACCTGCACCTGCGCCTTGACCAGCCGCATGAAAGCGCCGACTACCTGTGCACTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCca                                                                                                                                   <  5:58937/149‑1 (MQ=255)
               cGCCCACCACAACCTGGCTGACCTGCACCTGCGCCTTGACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCca                                                                                                                                   >  7:178378/1‑147 (MQ=255)
                    accacAACCTGGCTGACCTGCACCTGCGCCTTGACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTgcg                                                                                                                            <  6:170384/149‑1 (MQ=255)
                                cTGACCTGCACCTGCGCCTTGACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACAcc                                                                                                                <  2:182437/149‑1 (MQ=255)
                                   acctgcacctgcGCCTTGACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAg                                                                                                             <  8:39383/149‑1 (MQ=255)
                                   acctgcacctgcGCCTTGACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAg                                                                                                             <  7:246998/149‑1 (MQ=255)
                                          cctgcGCCTTGACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGa                                                                                                      <  5:254363/149‑1 (MQ=255)
                                          cctgcGCCTTGACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCAAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAgctgct                                                                                                        >  6:35370/1‑147 (MQ=255)
                                             gcgcCTTGACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGtt                                                                                                   >  7:42519/1‑149 (MQ=255)
                                               gcCTTGACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTc                                                                                                 <  6:129397/149‑1 (MQ=255)
                                                    gACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGc                                                                                            >  5:315794/1‑149 (MQ=255)
                                                    gACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCATCACGCAGcccctg                                                                                                                                       >  2:308124/1‑102 (MQ=255)
                                                    gACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCATCACGCAGcccctg                                                                                                                                       <  1:308124/106‑5 (MQ=255)
                                                       cAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGa                                                                                         <  7:175764/149‑1 (MQ=255)
                                                                      gcCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTAccc                                                                          <  8:178378/149‑1 (MQ=255)
                                                                      gcCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTAccc                                                                          <  4:314142/149‑1 (MQ=255)
                                                                          aCTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGcc                                                                                                                                           <  2:294862/80‑1 (MQ=255)
                                                                          aCTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGcc                                                                                                                                           >  1:294862/1‑80 (MQ=255)
                                                                             aCCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTAcc                                                                   >  8:2637/1‑149 (MQ=255)
                                                                               cTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTAcc                                                                   >  7:224812/1‑147 (MQ=255)
                                                                                tgtgCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATgg                                                                                   <  6:173484/130‑1 (MQ=255)
                                                                                tgtgCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATgg                                                                                   >  5:173484/1‑130 (MQ=255)
                                                                                  tgCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAgcg                                                              <  4:183369/149‑1 (MQ=255)
                                                                                       tgctCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGctgctg                                                                                                       <  2:245424/103‑1 (MQ=255)
                                                                                       tgctCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGctgctg                                                                                                       >  1:245424/1‑103 (MQ=255)
                                                                                         ctCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCt                                                        >  3:126310/1‑148 (MQ=255)
                                                                                              cAGCGACTGCTGCAGGCCTGCGAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGTGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTAcc                                                                   <  8:103884/132‑1 (MQ=255)
                                                                                              cAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCg                                                  >  1:96410/1‑149 (MQ=255)
                                                                                                   aCTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGa                                                                 <  4:16673/129‑1 (MQ=255)
                                                                                                   aCTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGa                                                                 >  3:16673/1‑129 (MQ=255)
                                                                                                   aCTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCcaca                                             <  7:2637/149‑1 (MQ=255)
                                                                                                       ctgcAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAgcgc                                         >  1:205006/1‑149 (MQ=255)
                                                                                                        tgcAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCa                                                      >  8:644/1‑135 (MQ=255)
                                                                                                        tgcAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCa                                                      <  7:644/135‑1 (MQ=255)
                                                                                                             gCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGc                                   <  6:315794/149‑1 (MQ=255)
                                                                                                                tGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGa                                                                 <  1:158646/116‑1 (MQ=255)
                                                                                                                tGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGa                                                                 >  2:158646/1‑116 (MQ=255)
                                                                                                                  ccAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGc                               <  3:254268/148‑1 (MQ=255)
                                                                                                                  ccAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCg                              >  3:342199/1‑149 (MQ=255)
                                                                                                                   cAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGc                               >  8:192233/1‑147 (MQ=255)
                                                                                                                   cAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGa                             <  2:96410/149‑1 (MQ=255)
                                                                                                                        aGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCg                        <  1:209101/149‑1 (MQ=255)
                                                                                                                          tGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCt                      <  5:35370/149‑1 (MQ=255)
                                                                                                                            cgcCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTg                     <  7:192233/148‑1 (MQ=255)
                                                                                                                                    tCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTg                                                          <  3:2513/103‑1 (MQ=255)
                                                                                                                                    tCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTg                                                          >  4:2513/1‑103 (MQ=255)
                                                                                                                                     gcaaggCTGCATCACGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGa           >  1:247019/5‑149 (MQ=255)
                                                                                                                                      aGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCgg            <  8:224812/147‑1 (MQ=255)
                                                                                                                                        gggCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGt        <  4:126310/149‑1 (MQ=255)
                                                                                                                                        gggCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGt        <  8:42519/149‑1 (MQ=255)
                                                                                                                                                 gATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACAcc  >  8:260416/1‑146 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GGCTGCATTCGTCATCGCCCACCACAACCTGGCTGACCTGCACCTGCGCCTTGACCAGCCGCATGAAAGCGCCGACTACCTGTGCGCTGCTCACCAGCGACTGCTGCAGGCCTGCCAGGAAGTGCGCCTGCCTCAGGGGCTGCGTGATGCAGCCTTGCGCCACAGTGCGCGCACTTACACCGAGCTGCTGAGTTTCATTGCCGAGTATGGCCAGTACCCGCGTACCGAGCGCTTGCTCAACCGCCACAGCGCCGGGGCCAGCGAGCTCGCTGCCAAGCCGGATGTAACACC  >  NC_002947/1090690‑1090980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑