Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,098,297 G→A 9.7% G94G (GGC→GGT)  kdsC ← 3‑deoxy‑D‑manno‑octulosonate 8‑phosphate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,098,2970GA 9.7% 111.6 / 2.5 41G94G (GGC→GGT) kdsC3‑deoxy‑D‑manno‑octulosonate 8‑phosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base G (19/18);  new base A (2/2);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

GCGTGCTGGCGTACGAACGGTGCGGCGTTGGCTACGGCCATGCCCAGAGCCACCCGGCGAATCACCGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCTGATGATCGCGGTGGTCACGCCCGAGGCCATGAGCATCTTGATGCCCTGG  >  NC_002947/1098163‑1098444
                                                                                                                                      |                                                                                                                                                    
gCGTGCTGGCGTACGAACGGTGCGGCGTTGGCTACGGCCATGCCCAGAGCCACCCGGCGAATCACCGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGcaccac                                                                                                                                        <  6:148150/149‑1 (MQ=255)
        gCGTACGAACGGTGCGGCGTTGGCTACGGCCATGCCCAGAGCCACCCGGCGAATCACCGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGt                                                                                                                               <  8:153423/149‑1 (MQ=255)
           tACGAACGGTGCGGCGTTGGCTACGGCCATGCCCAGAGCCACCCGGCGAATCACCGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGtcct                                                                                                                            <  5:261747/149‑1 (MQ=255)
             cGAACGGTGCGGCGTTGGCTACGGCCATGCCCAGAGCCACCCGGCGAATCACCGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGtcctc                                                                                                                           <  4:103089/148‑1 (MQ=255)
                   gTGCGGCGTTGGCTACGGCCATGCCCAGAGCCACCCGGCGAATCACCGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCt                                                                                                                    >  7:265530/1‑149 (MQ=255)
                    tGCGGCGTTGGCTACGGCCATGCCCAGAGCCACCCGGCGAATCACCGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCtt                                                                                                                   >  5:17009/1‑149 (MQ=255)
                                tACGGCCATGCCCAGAGCCACCCGGCGAATCACCGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGAACCACCAATT‑TCTCCTCACGGCCTTGGTAGAGGTGCg                                                                                                       >  8:310852/1‑149 (MQ=255)
                                 aCGGCCATGCCCAGAGCCACCCGGCGAATCACCGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCgg                                                                                                      >  2:240148/1‑149 (MQ=255)
                                     ccATGCCCAGAGCCACCCGGCGAATCACCGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATa                                                                                                  >  8:22629/1‑149 (MQ=255)
                                           ccAGATCCAACCGGCGAATCACCGGCATGTCGGGGATGTCGTCGCCCAGGTAAGCCAATTGATCATAGCTTTCGCCCACTTCGGCGATCATGGCGTCAAGGACCACCAATTGTGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGa                                                                                              <  1:240148/148‑1 (MQ=255)
                                            cAGAGCCACCCGGCGAATCACCGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGt                                                                                           <  8:273382/149‑1 (MQ=255)
                                            cAGAGCCACCCGGCGAATCACCGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGt                                                                                           >  5:135616/1‑149 (MQ=255)
                                              gagCCACCCGGCGAATCACCGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTc                                                                                         <  7:163200/149‑1 (MQ=255)
                                                        gCGAATCACCGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGc                                                                               <  7:22629/149‑1 (MQ=255)
                                                            aTCACCGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTc                                                                           <  6:336530/149‑1 (MQ=255)
                                                             tCACCGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTg                                                                                                                  >  3:207249/1‑109 (MQ=255)
                                                             tCACCGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTg                                                                                                                  <  4:207249/109‑1 (MQ=255)
                                                                ccGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGAc                                                                        >  2:186487/1‑148 (MQ=255)
                                                                   gcaggtcgggcaggtcgTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACg                                                                    >  6:344585/1‑149 (MQ=255)
                                                                                gtcgtcGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGcc                                                       >  4:154395/1‑149 (MQ=255)
                                                                                       cccAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCtgat                                                <  1:186487/149‑1 (MQ=255)
                                                                                             tAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCTGATGATcgc                                          <  8:265530/149‑1 (MQ=255)
                                                                                               aGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCTGATGATcgcg                                         <  6:17009/148‑1 (MQ=255)
                                                                                                  cACTTGATCATAGCTTAGTCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGTATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGc                                                    <  8:258859/134‑1 (MQ=255)
                                                                                                  cACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGc                                                    >  7:258859/1‑134 (MQ=255)
                                                                                                     ttGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCTGATGATCGCGGTGGTCa                                  >  8:120448/1‑149 (MQ=255)
                                                                                                          cATAGCTTAGGCCCAGTTCGGCGAGCAGACCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCg                                                                                 <  4:200978/97‑1 (MQ=255)
                                                                                                          cATAGCTTAGGCCCAGTTCGGCGAGCAGACCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCg                                                                                 >  3:200978/1‑97 (MQ=255)
                                                                                                            tAGCTTAGGCCCAGTTCGGCGGGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCTGATGATCGCGGTGGTCACGccc                             >  2:176024/1‑147 (MQ=255)
                                                                                                                  aGGCCCAGTTCGGCGAGCAGACCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCTGATGATCGCGGTGGTCACGCCCGAGGCCAt                     >  2:251287/1‑149 (MQ=255)
                                                                                                                    gCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCTGATGATCGCGGTGGTCACGCCCGAGGCCAt                     >  5:327709/1‑147 (MQ=255)
                                                                                                                    gCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCTGATGATCGCGGTGGTCACGCCCGAGGCCAt                     >  1:21919/1‑147 (MQ=255)
                                                                                                                    gCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCCCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCtga                                                 >  5:341319/1‑119 (MQ=255)
                                                                                                                    gCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCCCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCtga                                                 <  6:341319/119‑1 (MQ=255)
                                                                                                                      ccAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCTGATGATCGCGGTGGTCACGCCCGAGGCCATGAGc                 <  3:154395/149‑1 (MQ=255)
                                                                                                                          ttCGGCGAGCAGACCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCTGATGATCGCGGTGGTCACGCCCGAGGCCATGAGCATCt             <  1:251287/149‑1 (MQ=255)
                                                                                                                            cGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCTGATGATCGCGGTGGTCACGCCCGAGGCCATGAGCATCtt            <  6:135616/148‑1 (MQ=255)
                                                                                                                             ggCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCTGATGATCGCGGTGGTCACGCCCGAGGCCATGAGCATCTTGa          <  7:120448/149‑1 (MQ=255)
                                                                                                                               cGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCTGATGATCGCGGTGGTCACGCCCGAGGCCATGAGCATCTTGATg        >  2:125569/1‑149 (MQ=255)
                                                                                                                                gAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCTGATGATCGCGGTGGTCACGCCCGAGGCCATGAGCATCTTGATGc       >  6:132410/1‑149 (MQ=255)
                                                                                                                                gAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCTGATGATCGCGGTGGTCACGCCCGAGGCCATGAGCATCTTGATGc       >  8:77635/1‑149 (MQ=255)
                                                                                                                                     ggCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGTATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCTGATGATCGCGGTGGTCACGCCCGAGGCCATGAGCATCTTGATGCCCTgg  <  1:125569/149‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                                    
GCGTGCTGGCGTACGAACGGTGCGGCGTTGGCTACGGCCATGCCCAGAGCCACCCGGCGAATCACCGGCAGGTCGGGCAGGTCGTCGCCCAGGTAAGCCACTTGATCATAGCTTAGGCCCAGTTCGGCGAGCAGGCCGTCAAGCACCACCAATT‑TGTCCTCCCGGCCTTGGTAGAGGTGCGGGATACCGAGGTTCCTGGCCCGGCGTTCGACCACGGGGGTCTTGCGCCCGCTGATGATCGCGGTGGTCACGCCCGAGGCCATGAGCATCTTGATGCCCTGG  >  NC_002947/1098163‑1098444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑