Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,100,208	T→A	10.8%	V132E (GTG→GAG) ‡	mlaF →	ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,100,208	0	T	A	10.8%	132.2 / 5.8	54	V132E (GTG→GAG) ‡	mlaF	ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (24/24);  new base A (3/3);  total (27/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGAAAGCAGATGGGCGTGCTGTTCCAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAGGACCCGATCGCCATG  >  NC_002947/1100062‑1100353                                                                                                                                                   |                                                                                                                                                   ccGAAAGCAGATGGGCGTGCTGTTCCAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTgg                                                                                                                                                  >  4:291860/1‑149 (MQ=255) ccGAAAGCAGATGGGCGTGCTGTTCCAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTgg                                                                                                                                                  >  2:16440/1‑149 (MQ=255)          gATGGGCGTGCTGTTCCAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCCACGGCCTgcagc                                                                                                                                         <  8:130853/149‑4 (MQ=255)          gATGGGCGTGCTGTTCCAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCCACGGCCTgca                                                                                                                                           <  5:306272/147‑2 (MQ=255)          gATGGGCGTGCTGTTCCAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCCACGGCCTgca                                                                                                                                           >  6:306272/1‑146 (MQ=255)                          cAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGGGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGAt                                                                                                                         <  3:213393/149‑1 (MQ=255)                          cAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGa                                                                                                                               >  1:100388/1‑143 (MQ=255)                          cAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGa                                                                                                                               <  2:100388/143‑1 (MQ=255)                           agagCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTGAGCTGGCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATg                                                                                                                        <  5:33749/149‑1 (MQ=255)                                           ccccGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCg                                                                                                        >  6:196022/3‑149 (MQ=255)                                            aCCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTg                                                                                                            <  8:172206/144‑1 (MQ=255)                                            aCCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTg                                                                                                            >  7:172206/1‑144 (MQ=255)                                             ccGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGt                                                                                                      >  7:285221/1‑149 (MQ=255)                                              cGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTg                                                                                                     >  4:215486/1‑149 (MQ=255)                                                 ccTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCa                                                                                                  >  2:92000/1‑149 (MQ=255)                                                   tCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGTCTGCGCGGTGCCATCGACCTGATGCCCGACGAg                                                                                                               <  8:321529/134‑1 (MQ=255)                                                   tCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGTCTGCGCGGTGCCATCGACCTGATGCCCGACGAg                                                                                                               >  7:321529/1‑134 (MQ=255)                                                     gATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGGGCATACTCAGCTGGCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑aa                                                                                             <  5:61744/149‑1 (MQ=255)                                                          gtTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGcc                                                                                        >  2:95811/1‑149 (MQ=255)                                                          gtTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGcc                                                                                        >  6:14517/1‑149 (MQ=255)                                                           ttCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTg                                                                                                     <  2:85134/136‑1 (MQ=255)                                                           ttCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACAAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTg                                                                                                     >  1:85134/1‑136 (MQ=255)                                                             cGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCTCCgt                                                                                      <  8:59844/148‑1 (MQ=255)                                                               agtaCGTCGCGTTCCCGTTGCGCGTGCATACTCAGGTCGCGGACCAAATGATCCGTGACTTGGTGTTGATGACGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCTtgtg                                                                                   <  2:288889/146‑1 (MQ=255)                                                                 aaCGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGgc                                                                                 <  2:294003/149‑1 (MQ=255)                                                                   cGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATg                                                                                                <  3:70663/133‑1 (MQ=255)                                                                   cGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATg                                                                                                >  4:70663/1‑133 (MQ=255)                                                                     tCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGc                                                                                                   <  7:291878/128‑1 (MQ=255)                                                                     tCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGc                                                                                                   >  8:291878/1‑128 (MQ=255)                                                                                ttGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATGAAAGCGCCGTGTGGCGCTGGCCCGGGCGa                                                                   <  1:16440/149‑1 (MQ=255)                                                                                     cgTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGccc                                                             <  1:95811/149‑1 (MQ=255)                                                                                      gTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCt                                                            <  1:92000/149‑1 (MQ=255)                                                                                        gCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCcga                                                                                                                   >  8:329743/1‑93 (MQ=255)                                                                                        gCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCcga                                                                                                                   <  7:329743/93‑1 (MQ=255)                                                                                                 gCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTg                                                           <  5:314488/139‑1 (MQ=255)                                                                                                 gCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTg                                                           >  6:314488/1‑139 (MQ=255)                                                                                                     tCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATc                                               <  5:196022/147‑1 (MQ=255)                                                                                                     tCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATc                                               <  5:14517/147‑1 (MQ=255)                                                                                                        gACGAAATGATCCCTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCCGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGCCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCAt                                          >  4:286310/1‑149 (MQ=255)                                                                                                                 aTCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTc                                            <  6:72158/138‑1 (MQ=255)                                                                                                                 aTCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTc                                            >  5:72158/1‑138 (MQ=255)                                                                                                                     gTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCg                             >  2:33571/1‑149 (MQ=255)                                                                                                                          aTCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATc                                               <  6:359328/126‑1 (MQ=255)                                                                                                                          aTCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATc                                               >  5:359328/1‑126 (MQ=255)                                                                                                                              tgtTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGc                    >  2:170804/1‑149 (MQ=255)                                                                                                                                     caccgcgCAGGCCCACGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAGGAcc             >  3:264230/7‑149 (MQ=255)                                                                                                                                        gCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAGGACCCGa          >  1:215343/1‑149 (MQ=255)                                                                                                                                        cgcgCAGGCCCACGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTtcgtc                       <  6:369923/133‑1 (MQ=255)                                                                                                                                        cgcgCAGGCCCACGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTtcgtc                       >  5:369923/4‑136 (MQ=255)                                                                                                                                          tGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAGGACCCGATc        >  3:293736/1‑149 (MQ=255)                                                                                                                                              ggCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTtc                          >  3:99768/1‑127 (MQ=255)                                                                                                                                              ggCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTtc                          <  4:99768/127‑1 (MQ=255)                                                                                                                                                ccGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAGGACCCGATCGCCATg  <  3:215486/149‑1 (MQ=255)                                                                                                                                                ccGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAGGACCCGATCGCCATg  <  1:33571/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CCGAAAGCAGATGGGCGTGCTGTTCCAGAGCGGCGCGTTGTTCACCGACCTCGATGTGTTCGAGAACGTCGCGTTCCCGTTGCGCGTGCATACTCAGCTGTCGGACGAAATGATCCGTGACATCGTGTTGATGAAGCTGCAGGCCGTGGGCCTGCGCGGTGCCATCGACCTGATGCCCGACGAGCTGTCCGGTGGCATG‑AAGCGCCGTGTGGCGCTGGCCCGGGCGATTGCCCTGGACCCGCAGATCCTCATGTACGACGAACCGTTCGTCGGCCAGGACCCGATCGCCATG  >  NC_002947/1100062‑1100353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑