Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,101,790	A→T	6.9%	K124M (AAG→ATG)	mlaD →	phospholipid ABC transporter substrate‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,101,790	0	A	T	6.9%	213.9 / 4.3	72	K124M (AAG→ATG)	mlaD	phospholipid ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (34/33);  new base T (3/2);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

GATTCCTACACCGGTCGGGTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTG  >  NC_002947/1101645‑1101936                                                                                                                                                  |                                                                                                                                                   gATTCCTACACCGGTCGGGTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGa                                                                                                                                                 >  2:127767/1‑149 (MQ=255)  aTTCCTACACCGGTCGGGTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGAt                                                                                                                                                >  3:323121/1‑149 (MQ=255)     ccTACACCGGTCGGGTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATgg                                                                                                                                              <  2:288242/148‑1 (MQ=255)      cTACACCGGTCGGGTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGt                                                                                                                                             <  4:4532/148‑1 (MQ=255)              gTCGGGTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAAccatc                                                                                                                                    <  4:213946/149‑1 (MQ=255)                   gTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGa                                                                                                                               <  5:121932/149‑1 (MQ=255)                   gTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGa                                                                                                                               >  2:84219/1‑149 (MQ=255)                    tGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAAccatcc                                                                                                                                   <  5:154117/144‑1 (MQ=255)                    tGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAAccatcc                                                                                                                                   <  5:355290/144‑1 (MQ=255)                    tGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAAccatcc                                                                                                                                   >  6:154117/1‑144 (MQ=255)                    tGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAAccatcc                                                                                                                                   >  6:355290/1‑144 (MQ=255)                            cAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGt                                                                                                                       <  4:240288/148‑1 (MQ=255)                              gCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAAccatccat                                                                                                                                 >  7:14989/1‑136 (MQ=255)                              gCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAAccatccat                                                                                                                                 <  8:14989/136‑1 (MQ=255)                               cTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTTGCAccatcctt                                                                                                                                 >  7:211175/1‑133 (MQ=255)                               cTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTTGCAccatcctt                                                                                                                                 <  8:211175/135‑3 (MQ=255)                                  gACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATg                                                                                                                                               <  4:169720/118‑1 (MQ=255)                                  gACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATg                                                                                                                                               >  3:169720/1‑118 (MQ=255)                                  gACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACt                                                                                                                >  5:170795/1‑149 (MQ=255)                                   aCAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTg                                                                                                               <  8:64580/149‑1 (MQ=255)                                    cAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTgg                                                                                                              >  5:235109/1‑149 (MQ=255)                                       gtcggtcgATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGc                                                                                                           >  6:367132/1‑149 (MQ=255)                                       gtcggtcgATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGc                                                                                                           >  8:274797/1‑149 (MQ=255)                                       gtcggtcgATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGc                                                                                                           >  7:245846/1‑149 (MQ=255)                                       gtcggtcgATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGc                                                                                                           >  6:25164/1‑149 (MQ=255)                                       gtcggtcgATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTGGCTTGGTGAGAAGTACATAGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCCTACATGACACACAGACGGCCCTGGTGc                                                                                                           >  4:239019/1‑149 (MQ=255)                                                   ccTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGa                                                                                               >  2:355835/1‑149 (MQ=255)                                                    cTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGAt                                                                                              <  4:168626/149‑1 (MQ=255)                                                       ccgaccgaCTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTg                                                                                            <  2:285055/148‑1 (MQ=255)                                                            cgacTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGt                                                                                      >  8:192749/1‑149 (MQ=255)                                                                  cACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTg                                                                                                         <  7:90406/124‑1 (MQ=255)                                                                  cACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTg                                                                                                         >  8:90406/1‑124 (MQ=255)                                                                  cACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGc                                                                                >  2:152450/1‑149 (MQ=255)                                                                  cACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGc                                                                                >  2:288035/1‑149 (MQ=255)                                                                  cACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGc                                                                                <  6:170795/149‑1 (MQ=255)                                                                   aCTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCt                                                                               <  7:274797/149‑1 (MQ=255)                                                                    cTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTc                                                                              <  8:245846/149‑1 (MQ=255)                                                                     tGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTc                                                                              >  4:282745/1‑148 (MQ=255)                                                                       ccTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCg                                                                                                                     <  6:297568/107‑1 (MQ=255)                                                                       ccTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCg                                                                                                                     >  5:297568/1‑107 (MQ=255)                                                                           gATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAActc                                                                         <  4:323121/147‑1 (MQ=255)                                                                               cTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGAtt                                                                                             >  1:138550/1‑123 (MQ=255)                                                                               cTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGAtt                                                                                             <  2:138550/123‑1 (MQ=255)                                                                               cTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTgg                                                                   >  1:320780/1‑149 (MQ=255)                                                                                  aCCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCaa                                                                <  5:25164/149‑1 (MQ=255)                                                                                   ccgccgGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCaa                                                                <  2:320780/148‑1 (MQ=255)                                                                                   ccgccgGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAg                                                               <  1:152450/149‑1 (MQ=255)                                                                                    cgccgGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAg                                                               >  8:304974/1‑148 (MQ=255)                                                                                               ttGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAAc                                                           >  8:297625/1‑141 (MQ=255)                                                                                               ttGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAAc                                                           <  7:297625/141‑1 (MQ=255)                                                                                                 ggTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAaagaag                                                  <  7:192749/148‑1 (MQ=255)                                                                                                 ggTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAaagaa                                                   <  5:367132/147‑1 (MQ=255)                                                                                                 ggTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAaagaa                                                   <  6:235109/147‑1 (MQ=255)                                                                                                        aGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTCGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCg                                          >  1:150567/1‑149 (MQ=255)                                                                                                         gTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCgg                                         <  1:355835/149‑1 (MQ=255)                                                                                                                ggCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAAtt                                   <  1:288035/148‑1 (MQ=255)                                                                                                                  cATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAg                                >  8:271932/1‑149 (MQ=255)                                                                                                                   aTCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAg                                >  3:223417/1‑148 (MQ=255)                                                                                                                                 tGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGAttt                 <  7:271932/149‑1 (MQ=255)                                                                                                                                     gACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGAt             <  3:282745/149‑1 (MQ=255)                                                                                                                                     gACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGAt             >  7:206331/1‑149 (MQ=255)                                                                                                                                      aCGAAGTGCTCAAGGATGGTGCAACCATCCATGCCACACAGACGGCACTGGTGCTCGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGGTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCAACATATCGaac            >  5:355868/1‑147 (MQ=255)                                                                                                                                      aCGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGaa                                                            >  1:310048/1‑101 (MQ=255)                                                                                                                                      aCGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGaa                                                            <  2:310048/101‑1 (MQ=255)                                                                                                                                        gAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCt          >  4:207800/1‑149 (MQ=255)                                                                                                                                            gtgtCATGGATGGTTGCACCATCCTTGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGAttt                 <  1:191203/135‑1 (MQ=255)                                                                                                                                            gtgtCATGGATGGTTGCACCATCCTTGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGAttt                 >  2:191203/4‑138 (MQ=255)                                                                                                                                            gtgtCATGGATGGTTGCACCATCCTTGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGa      >  7:87444/4‑149 (MQ=255)                                                                                                                                               tCATGGATGGTTGCACCATCCTTGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAaagaa                                                   <  4:118691/101‑1 (MQ=255)                                                                                                                                               tCATGGATGGTTGCACCATCCTTGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAaagaa                                                   >  3:118691/1‑101 (MQ=255)                                                                                                                                               tCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATc            >  5:250150/1‑140 (MQ=255)                                                                                                                                               tCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATc            <  6:250150/140‑1 (MQ=255)                                                                                                                                                 aaGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTg  <  4:223417/148‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   GATTCCTACACCGGTCGGGTGACGTTGCAGCTGGACAAGTCGGTCGATAACCTGCCGACCGACTCCACTGCCTCGATCCTGACCGCCGGTTTGCTTGGTGAGAAGTACATCGGCATCAGCGTTGGCGGTGAAGACGAAGTGCTCAAGGATGGTGCAACCATCCATGACACACAGTCGGCACTGGTGCTGGAGGATCTGATTGGCAAGTTCCTGCTCAACTCTGTTGGCAAGGAACCGAAAGAAGCGCAACCGGCTAATTAAGGAGTACCCATGATTTCGATCCTGCGACGTG  >  NC_002947/1101645‑1101936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑