Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,121,444 C→T 9.4% V295V (GTC→GTT)  PP_0982 → YjgP/YjgQ family permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,121,4440CT 9.4% 147.4 / 4.5 53V295V (GTC→GTT) PP_0982YjgP/YjgQ family permease
Reads supporting (aligned to +/- strand):  ref base C (22/26);  new base T (3/2);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

GTGACCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTGTT  >  NC_002947/1121304‑1121590
                                                                                                                                            |                                                                                                                                                  
gTGACCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCcgcg                                                                                                                                            >  6:273005/1‑149 (MQ=255)
   aCCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTTGCGGTGCCGCTGTCGCGGGTCAACCCGCGcc                                                                                                                                          <  6:99707/148‑1 (MQ=255)
   aCCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGcc                                                                                                                                          <  1:229598/148‑1 (MQ=255)
        gcgcGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGcc                                                                                                                                    >  8:222331/1‑149 (MQ=255)
            gAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGTTGTTCGTCGTCACCCTGATGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCt                                                                                                                                 >  7:143197/1‑148 (MQ=255)
            gAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGA‑GGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTc                                                                                                                               <  7:168118/149‑1 (MQ=255)
             aaGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTc                                                                                                                               <  7:177987/149‑1 (MQ=255)
                cgcTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTc                                                                                                                            <  8:80108/149‑1 (MQ=255)
                      ccACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGCCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgctg                                                                                                                      >  4:133793/1‑149 (MQ=255)
                        aCCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgctgct                                                                                                                    <  8:305937/149‑1 (MQ=255)
                        aCCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAgctgct                                                                                                                    >  5:237256/1‑149 (MQ=255)
                             cGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTTGACCCGCGACAgc                                                                                                                                       >  5:95215/1‑124 (MQ=255)
                             cGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTTGACCCGCGACAgc                                                                                                                                       <  6:95215/125‑2 (MQ=255)
                             cGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCgg                                                                                                               <  1:332312/149‑1 (MQ=255)
                                   gATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTc                                                                                                                               <  8:304316/127‑1 (MQ=255)
                                   gATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTc                                                                                                                               >  7:304316/1‑127 (MQ=255)
                                   gATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAAttc                                                                                                         >  7:231920/1‑149 (MQ=255)
                                   gATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAAttc                                                                                                         >  7:173723/1‑149 (MQ=255)
                                    aTCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATGCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAAttc                                                                                                         <  2:1876/148‑1 (MQ=255)
                                     tCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAAttctt                                                                                                       <  5:273005/149‑1 (MQ=255)
                                        gCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAg                                                                                                                         <  2:283612/128‑1 (MQ=255)
                                        gCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAg                                                                                                                         >  1:283612/1‑128 (MQ=255)
                                        gCAAGGAGGGCCTGCGTGAGAGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTg                                                                                                    >  2:98053/1‑149 (MQ=255)
                                          aaGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTa                                                                                                  <  2:171/149‑1 (MQ=255)
                                             gaggGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATtcttct                                                                                                     >  5:259241/1‑143 (MQ=255)
                                             gaggGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATtcttct                                                                                                     <  6:259241/143‑1 (MQ=255)
                                                  ccTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGcc                                                                                           >  8:221549/1‑148 (MQ=255)
                                                  ccTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCt                                                                                          <  8:231920/149‑1 (MQ=255)
                                                  ccTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGGCGCGGGTTGACCCGCGACAg                                                                                                                                        <  7:103647/103‑1 (MQ=39)
                                                  ccTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGGCGCGGGTTGACCCGCGACAg                                                                                                                                        >  8:103647/1‑103 (MQ=39)
                                                      cGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCt                                                                                      <  8:173723/149‑1 (MQ=255)
                                                               gcCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCt                                                                             <  7:221549/149‑1 (MQ=255)
                                                                     cTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTc                                                                       <  4:12784/149‑1 (MQ=255)
                                                                             gCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTAcgcg                                                               >  2:121976/1‑149 (MQ=255)
                                                                              cGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACgcgg                                                              <  7:222331/149‑1 (MQ=255)
                                                                                   gTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACgcggctca                                                          <  1:98053/148‑4 (MQ=255)
                                                                                          ccGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCgag                                                    >  7:123220/1‑147 (MQ=255)
                                                                                           cGATCCTGGTGTTCGTCGTCACCCTGCTGGAGGTGCCGATGTCGCGGGTCAACCCGCTCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAg                                                 <  8:143197/149‑1 (MQ=255)
                                                                                                          tcgtcACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATc                                  >  1:153804/1‑149 (MQ=255)
                                                                                                            gtcACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGc                                >  6:230436/1‑149 (MQ=255)
                                                                                                                 ccTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTgg                           <  3:133793/149‑1 (MQ=255)
                                                                                                                 ccTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTgg                           <  2:153804/149‑1 (MQ=255)
                                                                                                                     ctgGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCAt                       >  7:325856/1‑149 (MQ=255)
                                                                                                                         cGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCCAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATgtgg                   <  8:123220/149‑1 (MQ=255)
                                                                                                                          ggTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATgtggt                  <  1:121976/149‑1 (MQ=255)
                                                                                                                            tGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGtggtgg                <  5:230436/149‑1 (MQ=255)
                                                                                                                                gCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTc             >  5:362336/1‑148 (MQ=255)
                                                                                                                                  tggcgcgGGTTGACCCGCGACAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCAc          >  1:33271/4‑149 (MQ=255)
                                                                                                                                      gcgGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGc                         >  6:97053/1‑130 (MQ=255)
                                                                                                                                      gcgGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGc                         <  5:97053/130‑1 (MQ=255)
                                                                                                                                      gcgGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGcc      >  1:36391/1‑149 (MQ=255)
                                                                                                                                       cgGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCt     >  8:203440/1‑149 (MQ=255)
                                                                                                                                          gTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTGtt  >  6:28287/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
GTGACCGAGCGCGAAGCGCTTCCCACCTCCGAACTGATCGGCAAGGAGGGCCTGCGTGAGCGCGCCGAGCTGCAATGGCGGTTGTCGCTGCCGATCCTGGTGTTCGTCGTCACCCTGCTGGCGGTGCCGCTGTCGCGGGTCAACCCGCGCCAGGGCCGCTTCCTCAAGCTGCTGCCGGCAATTCTTCTGTACATGGCCTACCTGACAATGCTGATTTCCGTACGCGGCGCCCTCGAGAAGGGCAAACTGCCGATCGCCCTGGGCATGTGGTGGGTCCACGGCCTGTT  >  NC_002947/1121304‑1121590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑